Báo cáo khoa học: Fidelity of hepatitis B virus polymerase
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Although efficient vaccines are available, chronic hepatitis B (HBV) infection poses a major health problem worldwide, and prolonged treatment of chronicallyinfected HBV patients with nucleoside analogs often results in drug-resistantHBVvariants.Therefore, it is critical toevaluate the contribution of the HBV polymerase to mutations. FLAG-taggedwild-type (FPolE) andmutant (FPolE/D551A)HBV polymerases have been expressed in insect cells and purified. The purified FPolE showed DNA polymerase activity, but FPolE/D551A did not, implying that the activity was derived from FPolE....
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