Establishing a procedure for detecting miRna-425-5p in human plasma using the RT-qPCR methodology

Quantitative reverse transcription PCR (RT-qPCR) paired with stem-loop was utilized in this study to establish a process for detecting miRNA-425-5p in human plasma, contributing to accurate prognosis and early diagnosis of genetic diseases in humans, especially cancer diseases. Blood samples (n=12) collected from healthy volunteers in Ho Chi Minh City, were subjected to plasma extraction and then microRNA (miRNAs) isolation using the TRIzol method.