Improving propagation of the rare plant Huperzia squarrosa using cuttings and in vitro techniques

The tassel fern, Huperzia squarrosa, is a rare and medicinally valuable plant known for containing Huperzine A. It propagates naturally through spores, rhizomes, cuttings, and clump division, but with a slow multiplication rate. This study aimed to optimize propagation conditions for H. squarrosa using stem cuttings and in vitro culture techniques to support its preservation and development.