Chapter 6 - DNA structure, replication, and recombination. This chapter includes contents: Experimental evidence for DNA as the genetic material, the Watson and Crick double helix model of DNA, genetic information in DNA base sequence, DNA replication, recombination at the DNA level.
Forensic DNA Typing charts the progress and development of DNA applied to
criminal forensics, providing vivid demonstrations of the amazing potential of
the method, not only to convict the guilty but also to exonerate the innocent.
John Butler has created a text that caters to all audiences, covering the basics
of DNA structure and function and describing in detail how the techniques are
used. In addition, the extensive use of D.N.A. (Data, Notes, and Application)
Boxes in the text enables the reader to dip in and out as he or she pleases....
Since the discovery of the DNA structure researchers have been highly interested in the molecular basis of genome inheritance. This book covers a wide range of aspects and issues related to the field of DNA replication. The association between genome replication, repair and recombination is also addressed, as well as summaries of recent work of the replication cycles of prokaryotic and eukaryotic viruses. The reader will gain an overview of our current understanding of DNA replication and related cellular processes, and useful resources for further reading....
T4 DNA ligase is one of the workhorses of molecular biology and used in
various biotechnological applications. Here we report that this ligase,
unlikeEscherichia coliDNA ligase,TaqDNA ligase and Ampligase, is able
to join the ends of single-stranded DNA in the absence of any duplex
DNA structure at the ligation site.
(BQ) Part 2 book "Lippincott's illustrated Q&A review of biochemistry" presents the following contents: Biochemical compounds, protein structure and function; DNA structure, replication and repair; RNA synthesis; protein synthesis, protein synthesis, molecular medicine and techniques, energy metabolism overview,...
The study of DNA advanced human knowledge in a way comparable to the major theories in physics, surpassed only by discoveries such as fire or the number zero. However, it also created conceptual shortcuts, beliefs and misunderstandings that obscure the natural phenomena, hindering its better understanding. The deep conviction that no human knowledge is perfect, but only perfectible, should function as a fair safeguard against scientific dogmatism and enable open discussion. With this aim, this book will offer to its readers 30 chapters on current trends in the field of DNA replication.
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Determining promoter location based on DNA structure first-principles calculations...
Chapter 11 provides knowledge of DNA and Its role in heredity. In this chapter, we will address the following questions: What is the evidence that the gene is DNA? What is the evidence that the gene is DNA? How is DNA replicated? How are errors in DNA repaired? What are some applications of our knowledge of DNA structure and replication?
After completing this unit, you should be able to: Describe the experiments that first supported the hypothesis that a cell’s hereditary material is located in the nucleus; understand the theory and conclusions associated with the Griffith and Avery experiments using Pneumococcus and mice; explain the evidence that supports the identity of DNA as hereditary material;...
Lecture Microbiology - Chapter 7: Microbial genetics. This chapter presents the following content: Heredity, genetic information storage, DNA structure, DNA concepts, information transfer - gene expression, DNA replication, gene representation,...
DNA polymerase 3D structure. DNA polymerase (ADN polymeraza) là một enzyme tham gia chính vào quá trình nhân đôi DNA. Những enzyme này xúc tác cho quá trình polymer hóa cácdeoxiribonucleotide dựa trên trình tự của một chuỗi DNA khác. Sợi DNA mới được tạo thành sẽ bổ sung với sợi DNA khuôn theo nguyên tắc bổ sung.
The Dnmt3a DNA methyltransferase is responsible for establishing DNA
methylation patterns during mammalian development. We show here that
the mouse Dnmt3a DNA methyltransferase is able to transfer the methyl
group fromS-adenosyl-L-methionine (AdoMet) to a cysteine residue in its
catalytic center. This reaction is irreversible and relatively slow.
Rad51 and disrupted meiotic cDNA1 (Dmc1) are the two eukaryotic DNA
recombinases that participate in homology search and strand exchange
reactions during homologous recombination mediated DNA repair. Rad51
expresses in both mitotic and meiotic tissues whereas Dmc1 is confined to
The Nbs1 protein associates with Mre11 and Rad50 proteins to form the
Mre11–Rad50–Nbs1 complex, which plays an important role in the
intracellular signaling pathway activated in response to DNA damage.
Mutations in the genes for each of these three components of the Mre11–
Rad50–Nbs1 complex result in human diseases characterized by genomic
Neuronal PAS domain protein 2 (NPAS2) is a circadian rhythm-associated
transcription factor with two heme-binding sites on two PAS domains. In
the present study, we compared the optical absorption spectra, resonance
Raman spectra, heme-binding kinetics and DNA-binding characteristics of
the isolated fragment containing the N-terminal basic helix–loop–helix
(bHLH) of the first PAS (PAS-A) domain of NPAS2 with those of the
PAS-A domain alone.
Ionizing radiation, oxidative stress and endogenous DNA-damage pro-cessing can result in a variety of single-strand breaks with modified 5¢
and⁄or 3¢ ends. These are thought to be one of the most persistent
forms of DNA damage and may threaten cell survival. This study
addresses the mechanism involved in recognition and processing of
DNA strand breaks containing modified 3¢ ends.
The NMR structure of 2¢,5¢ d(GGGGCCCC) was deter-mined togain insights into the structural differences between
2¢,5¢-and 3¢,5¢-linked DNA duplexes that may be relevant
in elucidating nature’s choice of sugar-phosphate links to
encode genetic information. The oligomer assumes a duplex
with extended nucleotide repeats formed out of mostly
N-type sugar puckers. With the exception of the 5¢-terminal
guanine that assumes thesyn glycosyl conformation, all
other bases prefer theantiglycosyl conformation. Base pairs
in the duplex exhibit slide ()1.
Ascorbic acid is often regarded as an antioxidantin vivo,
where it protects against cancer by scavenging DNA-dam-aging reactive oxygen species. However, the detailed mech-anism of the action of ascorbic acid on genetic DNA is still
unclear. We examined the effect of ascorbic acid on the
higher-order structure of DNA through real-time observa-tion by fluorescence microscopy. We found that ascorbic
acid generates a pearling structure in single giant DNA
molecules, with elongated and compact regions coexisting
along a molecular chain. ...
We have recently expressed in bacteria the enzymatically active reverse transcriptase (RT) of bovine leukemia virus (BLV) [Perach, M. & Hizi, A. (1999) Virology 259, 176–189]. In the present study, we have studied in vitro two features of the DNA polymerase activity of BLV RT, the processivity of DNA synthesis and the ﬁdelity of DNA synthesis. These properties were compared with those of the well-studied RTs of human immunodeﬁciency virus type 1 (HIV-1) and murine leukaemia virus (MLV).
A splicing factor SF2/ASF is a natural substrate for the kinase activity of human topoisomerase I. This study demonstrates that SF2/ASF inhibits DNA cleavage by human topoisomerase I induced by the anti-cancer agent camptothecin. The inhibition is independent of the phosphorylation status of SF2/ASF. We show that the inhibition did not result from binding of SF2/ASF to DNA that would hinder interactions between topoisomerase I and DNA. Neither it
was a consequence of a loss of sensitivity of the enzyme to camptothecin.