Glycosaminoglycan chain analysis

Scientifi c interest and curiosity for proteoglycan research has dramatically increased over the past decades, these molecules being no more considered as a scaffold for the cells of a given tissue, but more as a reservoir for growth factors and cytokines modulating their activation status and turnover. For example, heparin and other related heparan sulfate molecules are increasingly being recognized as important modulators of many signaling pathways.

374p hotmoingay 04-01-2013 26 3   Download

The importance of proteoglycans for secretionof proteolytic enzymes was studied in the murine macrophage cell line J774. Untreated or 4b-phorbol 12-myristate 13-acetate (PMA)-stimulated macrophages were treated with hexyl-b-D-thioxyloside to interfere with the attachment of glycosaminoglycan chains to their respective protein cores. Activation of the J774 macrophages with PMA resulted in increased secretion of trypsin-like serine proteinase activity.

10p tumor12 20-04-2013 8 1   Download

Domain IV, consisting of about 230 residues, represents a particular protein module so far found only in lamininb1 andb2 chains. Both domains were obtained by recombi-nant production in mammalian cells. They showed a globular structure, as expected from electron microscopic examination of laminins. Fragmentb1IV was obtained as a monomer and a disul®de-bonded dimer, and both were modi®ed to50% by a single chondroitin sulfate chain attached to Ser721 of an SGD consensus sequence.

12p research12 23-04-2013 18 1   Download