Báo cáo: Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs ( Progress Report)

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Mục đích dự án là để xác định lý do tại sao có những thất bại vắc-xin, và điều tra các type huyết thanh của FMDV đang lưu hành tại Việt Nam để chính xác vắc xin có thể được sử dụng cho các type huyết thanh. Phòng thí nghiệm khu vực được thiết lập với các hóa chất và phương pháp để cho phép một khả năng chẩn đoán cho FMDV chẩn đoán và huyết thanh chẩn đoán. Chiến lược kiểm soát cho sự hiểu biết của bệnh LMLM dịch tễ học đã được thực hiện thông qua các hội thảo đào tạo thú...

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Nội dung Text: Báo cáo: Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs ( Progress Report)

Ministry of Agriculture & Rural Development Progress Report Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs. Date 30/06/2006
Table of Contents 1. Institute Information _____________________________________________ 3 2. Project Abstract ________________________________________________ 4 3. Executive Summary _____________________________________________ 4 4. Introduction & Background _______________________________________ 5 5. Progress to Date________________________________________________ 6 5.1 Implementation Highlights ____________________________________ 6 5.2 Smallholder Benefits_________________________________________ 7 5.3 Capacity Building ___________________________________________ 8 5.4 Publicity ___________________________________________________ 8 5.5 Project Management _________________________________________ 8 6. Report on Cross-Cutting Issues ___________________________________ 8 6.1 Environment _______________________________________________ 8 6.2 Gender and Social Issues_____________________________________ 8 7. Implementation & Sustainability Issues_____________________________ 9 7.1 Issues and Constraints_______________________________________ 9 7.2 Options____________________________________________________ 9 7.3 Sustainability _______________________________________________ 9 8. Next Critical Steps _____________________________________________ 10 9. Conclusion ___________________________________________________ 10 10. Statuatory Declaration ________________________________________ 13
1. Institute Information Project Name Regional Animal Health Centre, Ho Vietnamese Institution Chi Minh City (RAHC-HCMC), South Vietnam. Dr. Dong Manh Hoa Vietnamese Project Team Leader Australian Animal Health Australian Organisation Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Mr Chris Morrissy Australian Personnel 01/06/2005 Date commenced 01/06/2008 Completion date (original) Completion date (revised) 31/12/2005 – 30/06/2006 Reporting period Contact Officer(s) In Australia: Team Leader Mr Chris Morrissy +61 3 5227 5000 Name: Telephone: Diagnostic Virologist +61 3 5227 5555 Position: Fax: Supervisor Mammalian Virology Organisation Australian Animal Health chris.morrissy@csiro.au Email: Laboratory (AAHL), PMB 24, Geelong, 3213, Australia In Australia: Administrative contact Mr Chris Morrissy +61 3 5227 5000 Name: Telephone: Patents Contracts Officer +61 3 5227 5555 Position: Fax: Organisation Australian Animal Health chris.morrissy@csiro.au Email: Laboratory (AAHL), PMB 24, Geelong, 3213, Australia In Vietnam Dr. Dong Manh Hoa + 84 8 8568220 Name: Telephone: Director + 84 8 8569050 Position: Fax: Organisation Regional Animal Health rahchcmc@hcm.vnn.vn Email: Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam.
2. Project Abstract The projects purpose is to determine why there are vaccine failures, and to investigate what serotypes of FMDV are circulating in Vietnam so that the correct vaccines can be used for these serotypes. Regional laboratories are setup with the reagents and methods to allow a diagnostic capability for FMDV diagnosis and serology. Control strategies for understanding of FMD epidemiology have been implemented through veterinary and laboratory training workshops and laboratory testing for both virus typing and serosurveillance has begun. The project has highlighted the importance of having a laboratory network to identify what is happening in the field and how to prevent and control disease outbreaks. The pilot zones are established in provinces near the borders of Vietnam to study serotypes circulating in Vietnam and to determine their origin. The quality and collection of samples have increased giving more data on the FMD situation in Vietnam. Molecular epidemiological studies of the FMDV isolates in these provinces will provide insights to the effectiveness of border control and the origin of FMDV circulating in Vietnam each year. A diagnostic capacity for FMD would allow the early detection and identification of disease enabling better control of disease and help reduce loss of livestock and so productivity. 3. Executive Summary The main focus of this reporting period was to commence the project, begin the training of the field veterinarians in the South, Centre and North of Vietnam and also the training of laboratory staff. All laboratory equipment was purchased and supplied to the laboratories in Vietnam. All laboratory consumables and reagents were also supplied to the laboratories in Vietnam for the first 6 months of the project. AAHL staff prepared the information necessary for the training of field veterinarians. This workshop information and the lectures were used to run workshops for the field veterinarians in the South and Centre of Vietnam and this information was then transferred to Dr Hoa and the staff at Ho Chi Minh City Regional Laboratory who gave the third workshop in Hanoi. The field studies were planned and implemented through the veterinary workshops, the field studies began with the first sero- surveillance survey in the South and Centre of Vietnam and the collection of outbreak samples. The Regional Animal Health Centre in Ho Chi Minh City also visited each province to train the field veterinarians on how to collect samples and how to collect information necessary for the history of the animals on the villages in the project. One staff member from the Regional Animal Health laboratory and one staff member from NAVETCO attended AAHL for training in ELISA technology for the diagnosis of FMD and standardisation of the reagents to be used in the first year of the project. Two AAHL staff consultants carried out workshops for the field veterinarians in the South and Centre of Vietnam. The third veterinary workshop in North Vietnam was carried out by DAH staff. Two AAHL consultants also carried out a workshop for 20 participants from each of the laboratories in the project and the regional laboratories in South Vietnam, in ELISA technologies for diagnosis of FMD at the Regional Animal Health Centre in Ho Chi Minh City. An important part of the laboratory training was Quality Assurance in the laboratory to ensure tests will be run according to a standard protocol and to allow AAHL to audit the results from each laboratory and give the appropriate data to allow trouble shooting. Avian Influenza (AI) has extended the sero-surveillance in the first six months.
4. Introduction & Background Objectives of the project: 1. To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance. 2. To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies. Completing these objectives will improve the diagnostic capability of the Veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control. This will strengthen the profile of DAH which will play a vital role in making Vietnam more economically competitive. Improved animal health will lead to an increase in rural productivity though increased animal production and indirectly in increased crop production. Healthy animals will enable small farmers to be more competitive in the local market. Control of FMD and animal diseases in general will give poor farmers a more stable income stream and reduce their vulnerability to natural and economic problems. Establishing a diagnostic network which extends from the North to South Vietnam, from the laboratory to the farm level, reinforced by training and education, will give Vietnam a working model on which to base disease control. This will directly increase the competitiveness and productivity of the national agricultural system which includes the major areas of concern including the Mekong Delta and the Central Coast. Implementation Approach and Strategy The project approach is thought to be the most appropriate for developing an understanding of FMD epidemiology in Vietnam. The approach for technology transfer is well established at AAHL and has been successfully applied in previous projects in Vietnam, Thailand and Indonesia. The field studies and epidemiology and serosurveillance approaches have been designed and planned in conjunction with DAH to provide the maximum necessary information to demonstrate the FMD situation in Vietnam and the effectiveness of FMD vaccines. The diagnostic technologies that will be used in this approach are the standard diagnostic tests in use throughout the world to study FMD as directed by OIE. AAHL has a lot of experience with field surveys for prevalence of antibodies, as in the ACIAR projects in Laos and Thailand on FMD. The Philippines is another example where OIE standard diagnostic tests are being used to control and eradicate FMD.
5. Progress to Date 5.1 Implementation Highlights Main achievements for this reporting period were: • Training of laboratory staff at AAHL, one representative from each laboratory were trained in ELISA techniques for the detection of FMD antigen and antibody and the standardisation of reagents. Training was carried out under a quality system emphasizing the importance of Quality Assurance in the laboratory focusing on test maintenance, record keeping and data collection. • A consultant from AAHL completed training of HCMC-RAHC staff in cell culture techniques and established this technology there to be used to grow FMD isolates from the field. The growth of FMD isolates from the field is important to allow further analysis of FMD field isolates by PCR and sequencing, Again Quality Assurance, record keeping and data collection was emphasized. • Further training of field veterinarians and feedback of the results from first round of testing and shared the experience from the first round of sample collection with the field veterinary staff to increase the quality of collected samples. • A consultant from AAHL visited each laboratory to complete the transfer of ELISA technology to each laboratory and to supply the ELISA reagents. Implementation of a Quality Assurance system into the laboratory and the testing of sera in each laboratory was discussed. Training of staf at RAHC- HCMC in the use of molecular techniques for the detection of FMD and the sequencing of FMD isolates. Training of RAHC-HCMC staff in FMD serology using cell culture to carry out the Virus Neutralisation Test (VNT) • Sampling for second phase of sera collection completed for each province for epidemiological studies and serosurveillance. The collection of tissue samples from outbreaks continued to increase giving valuable information on the serotypes circulating in Vietnam • ELISA technology in use at collaborating laboratories in HCMC, Hanoi and Can Tho, for serotyping of FMD tissue samples from the field. Sera from first round of serosurveillance tested at RAHC-HCMC. • Virus isolation in use at RAHC-HCMC. Molecular technology and serology by VNT using cell culture has began to be used. • Supply of consumables and further reagents for testing of samples by FMD ELISA, molecular technology and cell culture.
Report from Vietnam: Activities from Jan 1st 2006 to May 30th 2006 1. Training No. Date & place Subject Person in charge Remark 1 02-18 Feb 06 1. Tissue culture ( BHK 21, Ms. Catherine 4 participants HCM city BHK vacc, PK15 , BTY, Pig William . kidney ,Lamb kidney, Lamb Dr. Long testis ) Dr.Phong 2. Using the above cells to Dr.Vu isolate FMDV in Vietnam and compare the sensitivity between the cells 2 30 March 1. Application of ELISA for Mr. Chris Morrissy 5 participants to 15 April 06 FMDV diagnosis (antigen and Ms. Linda from 4 partner AAHL antibody detection , Ag-ELISA laboratories 3ABC-ELISA, LP-ELISA, C- (NVDC, RAHC- ELISA ) HCMC, CAN 2. Operation under a Quality THO , DA Assurance system NANG ) and 3. Reagent standardisation Sub-DAH- HCMC. 2. Field activities 2.1 Visit 8 provinces in the project (Middle and South Vietnam), March 2006. - Transfer the equipment and documents (form and questionnaire) for sample collection. - Transfer money for sample collection. - Feed back the results from first testing round and share the experience on sample collection with the field veterinary staff to increase the quality of collected samples. - Staff in charge: Dr. Hoa, Dr. Vu , Dr. Ha ( RAHC-HCMC) Dr. Quan, Dr.Quang (RAHC-Da Nang) Dr. Thanh, Dr. Dung (RAHC-Can Tho) 2.1.2 Visit 2 provinces in the project (North Vietnam), March 2006. - Transfer the equipment and documents (form and questionnaire) for sample collection. - Transfer money for sample collection.
- Feed back the results from first testing round and share the experience on sample collection with the field veterinary staff to increase the quality of collected samples. - Staff in charge: Dr. Cam, Dr. Tung (NVDC- Hanoi) 2.1.3 Visit 3 laboratories in the Project ( NVDC-Ha Noi , RAHC-Can tho, Da nang) early May, 2006. - Transfer the ELISA reagents - Discuss the plan for second testing round and share experience on laboratory management focusing on laboratory Quality Assurance and test management. - Staff in charge: Mr. Chris Morrissy, Dr. Long. 2.2. Collect sera samples from 10 provinces for the second round of surveillance, 120 cattle sera and 120 pig sera from each province. - NVDC-Ha Noi: 2 provinces (Quang Ninh and Lang Son), total 480 sera - RAHC-Da Nang: 2 provinces (Kontum and Quang Nam), total 480 sera - RAHC-Can Tho: 2 provinces (An Giang, Kien Giang ), total 480 sera - RAHC-HCMC: 4 provinces (Binh Phuoc, Tay Ninh, Long An, Dong Thap), total 960 sera 2.3 Collection of tissue samples from outbreaks for FMDV serotyping - NVDC-Hanoi: 208 tissue samples from North Vietnam - RAHC-Can Tho: 34 tissue samples from Mekong Delta. - RAHC-HCMC : 390 tissue samples from the South Vietnam . 3. Laboratory Activities 3.1 National Vaterinary Diagnostic Center- Ha Noi (NVDC-Hanoi): - Testing on tissue samples for FMDV serotyping: 208 samples, 7 of them are serotype Asia 1 and 201 of them are Serotype O. - Testing on sera for FMDV antibody detection: 480 sera, on going . 3.2 Regional Animal Health Centre Da Nang (RAHC-Da Nang) - Testing on sera for FMDV antibody detection: 480 sera, on going . 3.3 Regional Animal Health Centre Can Tho (RAHC-Can Tho)
- Testing on tissue samples for FMDV serotyping : 34 samples, all of them are serotype O. - Testing on sera for FMDV antibody detection: 480 sera , on going . 3.4 Regional Animal Health Centre HCMC (RAHC-HCMC) - Testing on tissue samples for FMDV serotyping: 390 samples, 1 of them is serotype Asia 1, the remaining are serotype O. - Testing on sera for FMDV antibody detection: 960 sera , on going. - Isolation FMDV by BHK cell line to confirm 3 samples from NVDC- Hanoi, 1 of them is serotype Asia1 in pigs and 1 sample is serotype O and1 sample virus could not isolated. - Isolation FMDV by BHK cell line from 39 selected field samples, 1 was serotype Asia1 and 38 were serotype O. - Application of Virus Neutralisation Test (VNT) protocol (testing phase to setup VNT technology at RAHC-HCMC): • Virus titration for serotype O and A , two viruses for each serotype • VNT applied using the serum from vaccine trial on cattle - RT-PCR application (testing phase to setup molecular technology at RAHC-HCMC, May, 2006) • Extract RNA from virus serotype O, A and Asia 1, two viruses for each serotype • Make cDNA from these RNA • RT-PCR carried out using primers to detect serotype O and A • Sent cDNA and amplicons to AAHL for sequencing Reporter: Ngo Thanh Long Note: Further detail on activities for reporting period in log frame. 5.2 Smallholder Benefits All pig and cattle and producers are potential beneficiaries. Those that take up the advice and use vaccine according to recommendations will benefit financially through reduced losses due to death and disease in their cattle and pigs. The benefits of a more profitable farming operation flow to all family members. Farmers and district veterinarians will have improved knowledge and skill in disease prevention, knowledge on the selection of the right vaccine and improving the efficacy of vaccination
5.3 Capacity Building Training and education of field veterinarians in disease prevention, disease investigation and sample collection has been carried out in the North, Centre and South of Vietnam. This training has already shown an impact with an increase in quality of sample collection and number of samples collected and submitted to the laboratory. The project has provided training and technology transfer of FMD diagnostics to each laboratory involved in the project. Reagents and standard methods have been supplied to each laboratory giving them the diagnostic capability for FMDV diagnosis and serology using ELISA technology, this technology is now able to be practised at each laboratory. 5.4 Publicity The CARD AusAID project have received publicity through the training programs and also through the achievements so far in the project in understanding FMD in Vietnam. FMD is a disease on importance in Vietnam and this has put our project into the lime light. The project has been publicised through a press release in Australia and articles in news letters including the SEAFMD newsletter. 5.5 Project Management Implementation of the project is on schedule. AAHL has kept the project on target and has supported or requests from the Vietnamese partners. Training has been carried out in Australia and Vietnam with both partners benefiting from the information that is being generated. Implementation in Vietnam is also continuing to go well. Trainees have been supplied in a timely manner, from DAH. The field work has been organised well with data and samples being collected from farms in each pilot zone. Training and the transfer of information to other regional laboratories in Vietnam is also occurring. RAHC-HCMC will supply support to these laboratories. 6. Report on Cross-Cutting Issues 6.1 Environment No direct environmental impacts associated with project activities have been identified. On a broader scale, the intention is that pig and cattle farming will become more efficient through a reduction in animal death and disease. Environmental benefit will result through more efficient utilization of resources such as animal feed, the energy to provide animal feed and to run other aspects of the farm. 6.2 Gender and Social Issues Application of new diagnostic tests will improve the ability of regional and provincial diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and appropriate measures to be applied to control disease. These benefits will begin during the project and continue to accrue with continued application. The beneficiaries of this project will be both large and small farms and particularly smallholder farmers whose animals and incomes will be protected by better disease diagnosis, management and control. Since women at the village level are the primary animal handlers and managers, they will be major beneficiaries of the final outcome of better diagnosis and control of animal diseases.
7. Implementation & Sustainability Issues 7.1 Issues and Constraints DAH has been very busy controlling the AI outbreaks in Vietnam and this has made there workload increase dramatically, this have meant some delays and rescheduling of training field veterinarians, eg training in the North Vietnam rescheduled. Sampling has also been spread out over a longer time period as well but this has had no effect with all samples still being collected. The collection of samples was made more difficult by the need to train all veterinarians in each province in data collection, on how to collect samples, collection of blood and how to restrain cattle. This increased training need has highlighted the need for further training of field veterinarians throughout Vietnam in disease investigation techniques with a focus on sample collection and data collection. There is a need for further input in this area, large scale training of field veterinarians to ensure better knowledge and control of disease in Vietnam. This could be achieved though better collaboration of aid agencies in Vietnam especially those supplying training for AI. 7.2 Options The government of Vietnam is looking at increasing the support to DAH and has increased funding for AI diagnosis and is looking to do the same for FMD diagnosis. The increased funding for AI has seen laboratories updated with new equipment some of which will improve all disease diagnosis which includes FMD. The money available to improve AI diagnosis can improve all disease diagnosis if this money is used wisely, eg in training field veterinarians in AI diagnosis, the training should be general to cover all diseases including FMD, CSF and other diseases of importance in Vietnam. Also in improving AI diagnosis in laboratories the training should emphasize how improvement can be applied to all areas of diagnosis, eg quality assurance in the laboratory. 7.3 Sustainability The DAH laboratories appear to be well supported by the central government. The DAH laboratories are also receiving increased support from overseas funding agencies as part of the AI campaign to improve laboratory facilities. RAHC-HCMC is developing in confidence as an institution. This confidence is beginning to be passed on to other DAH laboratories. The DAH scientific staff are quick to understand new information and are energetic in its implementation. People have an obvious desire to do their jobs well. This desire is obvious in staff in RAHC- HCMC and other laboratories. The laboratories just need technical support to be encouraged to carry out the techniques that they need for diagnosis and research. The project is following a well tried model for successful technology transfer, of demonstration and teaching of the technology in the donor facility followed by supply of reagents for implementation by the trainees at their own initiative in the recipient laboratory, subsequently backed up by consultancy visits to the recipient laboratory for support and fine tuning. A useful indicator of likely success is the initiative and ability of the trainees to make a useful attempt at implementation by themselves. DAH and NAVETCO staff show a high level of involvement in this way. The AusAID funded CSFV project, that linked DAH, NaVetCo and AAHL prior to this project, is proving sustainable. NAVETCO and DAH not only maintains the diagnostic capability developed at that time, but on its own initiative and unassisted
by AAHL is conducting training courses and technology transfer to other labs in Vietnam. 8. Next Critical Steps In the next 6 months a meeting will be held at AAHL to review the project outputs and to examine the information collected so far in the project, The meeting will decide if the data collected from the field is giving DAH the necessary information to make discisions on FMD control. An AAHL empidemilogist will be involved in collating the data from the first 2 phases od serosurveillance and epipdemlogical data collection and begin exposing DAH staff to data analysis techniques. The third phase of the sero-surveillance for the collection of the samples will be implemented with any changes necessary. In the next 6 months we will finalise all of the testing of the samples collected and evaluate each laboratories capabilities. In the second 6 months of the project it was decided to bring forward the training of the staff from each laboratory to be held at AAHL. This training will be carried out at AAHL for 1 representative from each laboratory for training in ELISA techniques. A consultant from AAHL will complete training of the HCMC-RAHC in cell culture techniques and establish this technology there to be used to grow FMD isolates from the field. The growth of FMD isolates from the field is important to allow further analysis of these isolates by PCR and sequencing. 9. Conclusion The project activities are on schedule. The DAH laboratories are running the field trials on time, even though they have had increased workload with avian influenza outbreaks. It is expected that the second sero-surveillance survey will be much easier than the first. The first took a lot longer to do due to commitments with avian influenza and also understanding the problems associated with collecting samples in areas where samples had not been collected before. Field veterinarians needed to be trained in the collection of tissue samples from infected animals as well as bleeding animals. The staff at Regional Animal Health Centre, Ho Chi Min City have performed a excellent job in getting everything to run on schedule in this first six months. The project should continue to run on time and I expect no further problems especially how the collection procedures are in place. The staff at DAH are very eager to learn and this is making a very good environment for transferring technology. The laboratory at Da Nang has less experience and this laboratory will need much more contact with AAHL and the laboratory at RAHC-HCMC to achieve the transfer of the ELISA technology to this laboratory. The laboratory in Hanoi has the skills and is doing quite well in the application of the ELISA but this laboratory needs further contact with AAHL to increase their understanding of the application of this technology for FMD diagnosis and this also applies to the laboratory in Can Tho in the south. Can Tho is applying the technologies learnt. This project has proved to be an important project for Vietnam with the importance of FMD and this has meant good publicity for the AusAID CARD program. It has also meant an increase in requests for help in other areas of animal health with the success of this project in improving the skills and technology at RAHC-HCMC and NAVETCO and through our other project in CSF at NAVETCO and DAH and Duck Plague at NAVETCO. DAH is using this project as an example of how to approach FMD control.
10. Statutory Declaration STATUTORY DECLARATION COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT PROGRAM CARD Project Title: FMD - Improved surveillance capabilities for FMDV CARD Project Number: 072/04VIE We the undersigned hereby declare that during the period 01/06/2005 to 31/12/2005 we have delivered the following inputs to assist in implementation of the above project. 1. PERSONNEL INPUTS Australian Personnel Days in Days in Trips to Provided (Name) Vietnam Australia Vietnam Chris Morrissy (also 28 10 2 replaced Laurie Gleeson’s time in project) Peter Daniels 14 1 Lynda Wright (replaced 14 1 Brenda Van Der Heide in project ) Greer Meehan 10 Axel Colling 5 Catherine Williams 15 Total 56 40 4 Vietnamese Personnel Days in Vietnam Provided Dr.Bui Quang Anh 10 Dr.Hoang Van Nam 20 Dr.Tran Ngoc Thang 15 RAHC-HCMC Dr.Dong Manh Hoa 25 Ngo Thanh Long, DVM 25 Nguyen Truc Ha, Ms 20 Pham Phong Vu, DVM 25 Nguyen Ngo M. Triet, DVM 25 NVDC-HA NOI
Dr. To Long Thanh 15 Dr. Nguyen Thu Ha 20 Nguyen Tung, Ms 20 RAHC-DA NANG Tran Van Quan,DVM 15 Le Thanh Quang, DVM 20 Huynh Thi Manh, Ms 20 RAHC-CAN THO Nguyen Ba Thanh, Ms 15 Truong Kim Dung, Ms 20 Tran Quoc Phong, DVM 20 NAVETCO Dr.Tran Xuan Hanh 15 Nguyen Thu Hong, Ms 20 Nguyen Van Hung, Bc 20 2. EQUIPMENT AND OTHER SERVICES Equipment & Other Services Budget Limit Description Inverted Microscope 4000 Pipettes 5000 Microfuge 3000 Laptop 4000 4000 Reagents 8755 Sample Collection 2000 Molecular technologies 4000 Laboratory consumables 14000 Freight 5000 ELIZA work
Project Progress Against Proposed Objectives, Outputs, Activities And Inputs Project Title: Vietnamese Implementing Institution: PROPOSAL PROGRESS REPORT Narrative Information Required Performance Assumptions Information Required Measures OBJECTIVES To establish an effective Samples collected and Technology will be Establishment of an effective laboratory 1. laboratory network for the submitted to laboratory. taken up by network for the diagnosis and control of FMD diagnosis and control of FMD by Data produced and results laboratories. continued with training given at AAHL under a the provision of resources and feedback to field veterinary Accurate data Quality system, method established at each training of staff in required staff and farms. collected from farms. laboratory, QA introduced and resource methods and quality assurance. Little risk. supplied. The four DAH laboratories are (Objective 1.2.2, 2.1.1 & Strategy working together to carry out surveillance and 1) testing. Train field veterinary staff in Samples collected from Accurate data Training carried out in the North, Centre and 1.1 outbreak investigation for the field and submitted to collected from farms. South of Vietnam for veterinary field and investigation of cases of FMD on laboratory. Sample and Little risk laboratory staff. farms, including record keeping, farm records presented with sampling strategies and data samples. collection. Establish ELISA technology from Diagnostics test in use. QA Technology will be ELISA training and reagents delivered. 1.2 AAHL to collaborating controls meet. internal taken up by Laboratories have began to use the ELISA laboratories quality control sheets for laboratories. Little technology to test samples. examination, correct results risk in external proficiency testing organized by AAHL. Results generated.
Narrative Information Required Performance Measures Assumptions Information Required Using cell culture at RAHC- Virus isolates grown in cell Cell cultures can be Training at AAHL carried out and cells 1.3 HCMC, make a collection of culture maintained at RAHC- transferred to RAHC-HCMC. FMD samples isolates of FMD viruses from HCMC. Little risk. collected. Further training in HCMC this 6 cases of FMD disease on farms. months and cell culture established at RAHC- HCMC At RAHC-HCMC and AAHL use Sequence data produced. Technology will be FMD samples collected 1.4 molecular techniques to assess taken up by strains of FMD circulating laboratories. Little risk Introduce cell culture to NVDC- Cell culture in use at Technology will be Activity later in project 1.5 Hanoi for FMD diagnosis NVDC-Hanoi taken up by laboratories. Little risk Firm link with AAHL for the Diagnostic reagents used Reagents available. Process began 1.6 ongoing supply of diagnostic for routine diagnostics. Little risk. reagents. To provide accurate data to Project Report presented to Project objectives FMD samples collected 2. explain failure of vaccination to DAH and AusAID. meet. Little risk. control FMDV and to develop new Feedback meetings held in effective vaccine application provinces. strategies. (Objective 1.2.2, 2.1.1 & Strategy 1)
Narrative Information Required Performance Measures Assumptions Information Required Hold information and training Samples collected from Accurate data Training carried out in the North, Centre and 2.1 workshops for farmers and their field and submitted to collected from farms. South of Vietnam for veterinary field and veterinary advisors, with laboratory. Sample and Little risk laboratory staff. Further information to farmers emphasis on FMD prevention farm records presented with to be delivered later in project and disease investigation and samples. sample collection Pilot zones established in each Samples collected from DAH is able to have Pilot zones established. First round of 2.2 province. field and submitted to farmers agree to serosurveillance completed. laboratory. Sample and project. Little risk farm records presented with samples. Feedback to collaborating Results on laboratory Technology will be Activity later in project 2.3 laboratories results from test comparisons and EQA taken up by comparisons at AAHL and send back to each laboratories. Little RAHC-HCMC. laboratory. risk Feedback results to veterinary Results maintained at field Samples collected Activity later in project 2.4 field staff and farmers. veterinary office and tested. Little risk. Determine the efficacy of the Comparison of FMD Samples collected Activity later in project. Samples collected. 2.5 FMD vaccine in protecting genotypes to vaccine. and tested. Little risk. against FMD isolates circulating Results of serosurveillance. in Vietnam.
Hold information and training Meetings held with farmers Project will achieve Samples collected 2.6 workshops farmers and their and their veterinary objectives. veterinary advisors, with advisors Little risk. emphasis on FMD prevention and presenting the data obtained during the CARD project. OUTPUTS • Samples collected and Process began. Training carried out in the 1. & 2. Technology will be Field veterinarians that are submitted to laboratory. North, Centre and South of Vietnam for taken up by skilled in on farm diagnosis Data produced and results veterinary field and laboratory staff. laboratories. and sample collection. • feedback to field veterinary Activity later in project Accurate data Education of farmers to allow staff and farms. collected from farms. better understanding of the Little risk. benefits of diagnostic technologies. Training at AAHL carried out and cells • Development of cell culture transferred to RAHC-HCMC. FMD samples capability for virus collected propagation, isolation from field samples and for serology by virus neutralisation test (VNT). • Establishment of an effective laboratory Diagnostic capabilities at all network for the diagnosis and control of FMD the laboratories participating began with training given, method in the project to carry out established, QA introduced and resource functional tests as described supplied. under a Quality System. • Pilot zones established and Pilot zones established. First round of operational. serosurveillance completed. • Firm link with AAHL for the ongoing supply of diagnostic Process began. reagents. • Application of technologies, Activity later in project knowledge and skills to other
diseases. • Expected outcome at end of project Increased productivity by small farm holders with more food available to poor farmers. This will have a more pronounced effect on the women and children who are mainly responsible for the animals’ upkeep and likely to obtain the major benefits of increased crop production and sale of animals. • Field veterinarians trained in Samples collected from Accurate data 1.1-1.6 & on farm diagnosis and Training carried out in the North, Centre and field and submitted to collected from farms. 2.1- 2.6 sample collection. South of Vietnam for veterinary field and laboratory. Sample and Little risk laboratory staff. farm records presented with samples. • Education of farmers to allow Training carried out in the North, Centre and better understanding of the Farmers participating in DAH is able to have South of Vietnam for veterinary field and benefits of diagnostic projects farmers agree to laboratory staff. technologies to disease project. Little risk control. Pilot zones established. First round of • Setup farms/villages in each Samples collected from DAH is able to have serosurveillance completed. province for seroservalance field and submitted to farmers agree to laboratory. Sample and project. Little risk farm records presented with samples. • Transfer of ELISA technology Diagnostics test in use. QA Technology will be Establishment of an effective laboratory to collaborating laboratories controls met, internal taken up by network for the diagnosis and control of FMD quality control sheets laboratories. Little began with training given, method available for examination, risk established, QA introduced and resource correct results in external