Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells
Lysophosphatidicacid (LPA), oneof thenaturallyoccurring
phospholipids, stimulates cellmotility through theactivation
of Rho family members, but the signaling mechanisms
remain tobe elucidated. In thepresent study,we investigated
the roles of p21-activated kinase 1 (PAK1) onLPA-induced
focal adhesion kinase (FAK) phosphorylation and cell
motility. Treatment of human melanoma cells A2058 with
LPA increased phosphorylation and activation of PAK1,
which was blocked by treatment with pertussis toxin and
by inhibition of phosphoinositide 3-kinase (PI3K) with an
inhibitor LY294002 or by overexpression of catalytically
inactive mutant of PI3Kc, indicating that LPA-induced
PAK1 activation was mediated via a Gi protein and the
PI3Kcsignalingpathway....