Analysis of cytoplasmic

Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ chanThe nuclear geneMRS2in Saccharomyces cerevisiaeencodes an integral protein (Mrs2p) of the inner mitochondrial membrane. It forms an ion channel mediating influx of Mg 2+ into mitochondria. Orthologues of Mrs2p have been shown to exist in other lower eukaryotes, in vertebrates and in plants.nel protein of Saccharomyces cerevisiae ` Julian Weghuber, Frank Dieterich, Elisabeth M. Froschauer, Sona Svidova and Rudolf J. Schweyen Max F.

12p inspiron33 26-03-2013 43 3   Download

Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate specificity Nicola Barison1,2, Laura Cendron1,2, Alberto Trento2, Alessandro Angelini2,* and Giuseppe Zanotti1,2,3 1 Department of Biological Chemistry, University of Padua, Italy 2 Venetian Institute of Molecular Medicine (VIMM), Padua, Italy 3 Institute of Biomolecular Chemistry of CNR, Padua, Italy Keywords Helicobacter pylori; stomach colonization; thiamin; thiaminase; vitamin B1 Correspondence G.

9p viettel02 20-02-2013 24 2   Download

Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and peptides.The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures.In this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, i.e.

7p tumor12 20-04-2013 21 1   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis

8p hoami_238 17-09-2011 27 3   Download

MicroRNAs (miRNAs) have been proven to play important roles at the post-transcriptional level in animals and plants. To investigate clustering patterns and specific sequence characteristics in the flanking regions of plant miRNA genes, we performed genome-wide analyses ofArabidopsis thaliana, Populus trichocarpa, Oryza sativaand Sorghum bicolor.

12p cosis54 05-01-2013 32 2   Download

Upon mutation of Asn130 to aspartate, the catalytic activity of human arginase I was reduced to17% of wild-type activity, theKmvalue for arginine was increased9-fold, and thekcat ⁄Kmvalue was reduced50-fold. The kinetic properties were much less affected by replacement of Asn130 with glutamine.

7p inspiron33 23-03-2013 25 2   Download

Five healthy adult female first-generation hybrid tree frogs were produced by interspecific breeding of closely related tree frogsLitoria splendidaand L. caeruleain a cage containing large numbers of males and females of both species. Phylogenetic analysis of mitochondrial DNA sequences estab-lished the female parent to be L. splendida.

9p inspiron33 25-03-2013 22 2   Download

We reportedpreviously a direct correlationbetween reduced soybean agglutinin binding to 63- and 68-kDa midgut gly-coproteins and resistance to Cry1Ac toxin from Bacillus thuringiensisin the tobacco budworm (Heliothis virescens). In the present work we describe the identification of the 68-kDaglycoproteinas amembrane-bound formof alkaline phosphatase we term HvALP. Lectin blot analysis of HvALP revealed the existence of N-linked oligosaccharides containing terminal N-acetylgalactosamine required for [ 125 I]Cry1Ac binding in ligand blots. ...

9p awards 05-04-2013 30 2   Download

The structure of the lipid A and core region of the lipo-polysaccharide (LPS) fromFrancisella tularensis(ATCC 29684) was analysed using NMR, mass spectrometry and chemical methods. The LPS contains a b-GlcN-(1–6)-GlcN lipid A backbone, but has a number of unusual structural features; it apparently has no substituent at O-1 of the reducing end GlcN residue in the lipid part in the major part of the population, no substituents at O-3 and O-4ofb-GlcN, and no substituent at O-4of the Kdo residue.

7p tumor12 22-04-2013 25 2   Download

The Arabidopsis FAE1 b-ketoacyl-CoA synthase (FAE1 KCS) catalyzes the condensation of malonyl-CoA with longchain acyl-CoAs. Sequence analysis of FAE1 KCS predicted that this condensing enzyme is anchored to a membrane by two adjacent N-terminal membrane-spanning domains. In order to characterize the FAE1 KCS and analyze its mechanism, FAE1 KCS and its mutants were engineered with a His6-tag at their N-terminus, and expressed in Saccharomyces cerevisiae. The membrane-bound enzyme was then solubilized and purified to near homogeneity on a metal affinity column.

9p research12 01-06-2013 25 2   Download

RNase H2 ofSaccharomyces cerevisiaeconsists of three essential subunits (Rnh201, Rnh202 and Rnh203) and plays a critical role in the removal of RNA incorporated in duplex DNA. In the present study, we purified indi-vidual subunits and heterodimeric subcomplexes to examine the assembly and biochemical function of subunits of RNase H2in vitro.

16p cosis54 08-12-2012 30 1   Download

EVL is a member of the ENA⁄VASP family, which is involved in actin-remodeling processes. Previously, we reported that human EVL directly interacts with RAD51, which is an essential protein in the homologous recombinational repair of DNA double-strand breaks, and stimulates RAD51-mediated recombination reactionsin vitro.

8p viettel02 20-02-2013 22 1   Download

Human topoisomerase I binds DNA in a topology-dependent fashion with a strong preference for supercoiled DNAs of either sign over relaxed circu-lar DNA. One hypothesis to account for this preference is that a second DNA-binding site exists on the enzyme that mediates an association with the nodes present in supercoiled DNA.

14p viettel02 20-02-2013 16 1   Download

Aninterleukin-7 (IL-7) gene has been cloned and sequenced within the Japanese pufferfish,Takifugu rubripes(Fugu), providing the first conclusive evidence for the existence of IL-7 in teleosts. The IL-7 cDNA was com-posed of 1438 bp, with a 117 bp 5¢-UTR, an 826 bp 3¢-UTR containing two mRNA instability motifs, and a 498 bp ORF which translates into a peptide of 166 amino acid residues.

14p media19 06-03-2013 19 1   Download

A versatile software tool,virtualmslab, is presented that can perform advanced complex virtual proteomic experiments with mass spectrometric analyses to assist in the characterization of proteins. The virtual experimen-tal results allow rapid, flexible and convenient exploration of sample prepar-ation strategies and are used to generate MS reference databases that can be matched with the real MS data obtained from the equivalent real experi-ments.

11p dell39 27-03-2013 18 1   Download

A wide range of approaches has been applied to examine the quaternary structure of G protein-coupled receptors, the basis of such protein–protein interactions and how such interactions might modulate the pharmacology and function of these receptors. These include coimmunoprecipitation, var-ious adaptations of resonance energy transfer techniques, functional com-plementation studies and the analysis of ligand-binding data.

12p fptmusic 11-04-2013 32 1   Download

Spermadhesins are a family of 12–16 kDa proteins with a single CUB domain. PSP-I and PSP-II, the most abundant boar spermadhesins, are present in seminal plasma as a noncovalent heterodimer. Dimerization markedly affects the binding ability of the subunits. Notably, heparin and mannose 6-phosphate binding abilities of PSP-II are abolished, indicating that the corresponding binding sites may be located at (or near) the dimer interface.

8p fptmusic 12-04-2013 19 1   Download

Galactokinase (EC 2.7.1.6) catalyzes the first committed step in the catabolism of galactose. The sugar is phosphor-ylated at position 1 at the expense of ATP. Lack of fully functional galactokinase is one cause of the inherited disease galactosemia, the main clinical manifestation of which is early onset cataracts. Human galactokinase (GALK1) was expressed in and purified fromEscherichia coli.The recom-binant enzyme was both soluble and active.

8p fptmusic 16-04-2013 20 1   Download

Flexibacter maritimus, a Gram-negative bacterium, is a fish pathogenresponsible fordisease infinfishspecies andacause of cutaneous erosiondisease in sea-caged salmonids. For the development of serology based diagnostics, protective vac-cines, and a study of pathogenesis, the structural analysis of the lipopolysaccharide (LPS) producedby the bacteriumhas been undertaken. We now report that an acidic O-specific polysaccharide, obtained by mild acid degradation of the F. maritimusLPSwas found to be composed of a disac-...

6p fptmusic 16-04-2013 24 1   Download

Two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, were identified in two and one spots, respectively, in a proteome analysis of barley (Hordeum vulgare) seeds based on 2D gel electrophoresis and MS. HvTrxh1 was observed in 2D gel patterns of endosperm, aleurone layer and embryo of mature barley seeds, andHvTrxh2was presentmainly in the embryo. During germination, HvTrxh2 decreased in abun-dance and HvTrxh1 decreased in the aleurone layer and endospermbut remainedat high levels in the embryo.

11p fptmusic 16-04-2013 29 1   Download