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Báo cáo lâm nghiệp: "Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase"
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Nội dung Text: Báo cáo lâm nghiệp: "Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase"
- Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase C. Dumas 2 2 Gaget M. Villar M. 1 INRA, 1 Station D’Amélioration des Arbres Forestiers, Ardon, F-45160 Olivet, and dAm6lioration Laboratoire 2 de Reconnaissance Cellulaire et Am6lioration des Plantes, INRA 23879, Université Lyon I, 43, bd 11-Novembre-1918, F-69622 Villeurbanne Cedex, France tions, silver nitrate staining and homogenates of Introduction pollinated stigma performed according to were the procedures ot’ Villar et al. (1988). Each of the 2 crosses was represented by 3 series of Hybridization in Populus breeding pro- stigmatic extract!., in accordance with the grams is limited by sexual incompatibility growth kinetics (0.6 and 20 h after pollination). barriers, whose cellular and molecular Protein patterns of these extracts were com- mechanisms are not yet known. In an pared on a single gel. Glycoprotein revelation (concanavalin A-binding proteins) on IEF poly- attempt to understand the nature of inter- acrylamide gel was adapted from Hawkes specific incompatibility in Populus, we (1982). j3-Ga!actosidase visualization was op- have explored the interactions between timized from the protocol of Singh and Knox male and female partners (pollen-pistil) in (1985). compatible and incompatible crosses P. nigra (female) x P. nigra (male) and P. nigra (female) x P. alba (male) (Villar, Results and Dis;cussion 1987). Kinetics of pollen tube growth (visualiza- tion using the AI3F method) have demon- Materials and Methods strated distinct behaviors of P nigra and P alba pollen tubes inside P nigra pistils (Fig. 1P alba pollen tubes exhibit a P. nigra and P. alba branches were obtained unique S-shaped growth curve and an from the INRA Forestry station in Or[6ans (France). Pollen was collected and stored in arrested growth near the stylodium. On closed vials at -18°C. Kinetics of pollen tube the other hand, P nigra pollen tubes ex- growth in vivo were studied in growth chambers hibit 2 growth phases, respectively, in the (20°C). Pollen tubes in the stylar tissues were stigmatic tissues and in the ovarian cavity. visualized using the aniline blue fluorescence P, nigra and P alba curves diverge 5 h method (ABF method, Dumas and Knox, 1983). after pollination (20°C), corresponding Isoelectric focusing (IEF) of pollinic and stig- matic proteins, gel preparation, focusing condi- precisely to the deposition of the first
- observed, Differences plug inside pollen tubes. This callose glycoproteins. were distinct type of to the divergence could likely be related to a according cross: one in the compat- increases change in the physiology of the compat- only glycoprotein ible cross. Moreover, f3-Galactosidase ac- ible pollen tube, shifting from an auto- tivities were revealed with a similar trophic to a heterotropic type of nutrition. electrophoretic technique in pollinated Biochemical studies focused on pollinic stigma. This pollinic enzyme could play a and stigmatic proteins, known to be in- role in heterotropic pollen tube nutrition volved in male-female interactions (Singh and Knox, 1985). An increase of its (Gaude and Dumas, 1987). After silver activity (one isoenzyme of isoelectric point nitrate staining, qualitative and quantita- about pH 4.2) from 6-20 h after pollination tive differences could be observed, related was detected only in the compatible cross. to the presence of P nigra and P alba pol- len tubes inside stigmatic tissues. How- ever, increasing protein bands were Conclusion detectable, 0-20 h after pollination, only in compatible pollinated stigmas. The conca- in P. The compatible progamic phase navalin A-peroxidase reaction allowed the to the nigra, i.e., pollen tube growth up visualization of 15 stigmatic and pollinic
- sac, could be related to pollinic Gaget M. (1988) incompatibility intersp6cifique embryo chez Populus: effet Mentor. Ph.D. Thesis, Uni- enzymes involved in pollen tube metabo- versite Lyon I, France lism, such as ¡3-galactosidase. Its activity Gaude T. & Dumas C. (1987) Molecular and could be the final result of a series of inter- cellular events of self-incompatibility. Int. Rev. actions started by initial pollen-stigma Cytol. 107,333-366 communications. This dialogue probably Hawkes R. (1982) Identification of concanavalin implicates protein compounds, detected in A-binding proteins after sodium dodecyl sul- pollen and pollen tube diffusates in vitro fate-gel electrophoresis and protein blotting. AnaL Biochem. 4 23, 143-146 (Villar, 1987; Gaget, 1988). Singh K. and Knox R.B. (1985) P-Galactosidase of Lilium pollen. Phytochemistry 24, 1639-1643 Villar M. (1987) Incompatibilit6 intersp6cifique chez Populus: approches physiologique et References biochimique. Phi.D. Thesis, Universit6 Lyon I, France Dumas C. & Knox R.B. (1983) Callose and Villar M., Gaget M. & Dumas C. (1988) Micro- determination of pistil viability and incompatibili- isoelectric focusing of proteins from single stig- ty. Theor. AppL Genet. 67, 1-10 ma in Populus. Can. J. For. 18, 1261-1264 0
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