Báo cáo Y học: Characterization of a partially folded intermediate of stem bromelain at low pH
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Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, ¯uorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stembromelain lacks awell de®ned tertiary structure as seen by ¯uorescence and near-UVCDspectra.Far-UVCDspectra showretentionof some native like secondary structure at pH 2.0. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, ¯uorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well de®ned tertiary structure as seen by...
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