Absorption spectrophotometry

The atomic absorption spectrometry uses absorption of light of intrinsic wavelengths by atoms. All atoms are classified into those having low energies and those having high energies. The state having low energies is called the ground state and the state having high energies is called the excited state. The atom in the ground state absorbs external energies and is put in the excited state. For example, sodium is mainly in two excited states, having higher energies by 2.2eV and 3.6eV respectively than in the ground state, as shown in Fig. 1.1.

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Atomic Absorption, Fundamentals and Applications, Different Regions, Absorption Spectrophotometry, Fresh and Deparaffinized, Antioxidant Properties

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Invite you to consult the document content "Chapter 8: Fundamentals of Spectrophotometry" to grasp the following: Properties of light, absorption of light, beer’s law in chemical analysis, exercises. Hope this is useful references for you.

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Metformin (1,1-dimethylbiguanide) is an antihyperglycae-mic drug used to normalize glucose concentrations in type 2 diabetes. Furthermore, antioxidant benefits have been reported in diabetic patients treated with metformin. This work was aimed at studying the scavenging capacity of this drug against reactive oxygen species (ROS) like Æ OH and O Æ
2 -free radicals. ROS were produced by gamma radio-lysis of water. The irradiated solutions of metformin were analyzed by UV/visible absorption spectrophotometry.

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Modern chemical analysis has routinely used spectrophotometry in agricultural, clinical, environmental, pharmaceutical, and quality control laboratories for over fifty years. Spectrophotometry is the study of absorption or emission of light by a chemical species

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The interaction of tungsten(VI) with CV has been investigated in acetone-aqueous medium. The complex has been examined by solvent extraction-spectrophotometry method. In optimum conditions (pH 3; 30% acetone v/v) the ion-association complex of tungsten(VI) with CV was formed with ratio of 2 : 1, and it was extracted quantitatively one time with 5 mL toluene. In organic phase, the complex has maximum absorption at 599 nm with = 11.2 x 104 L.mol -1 .cm-1 , and stable at least 2 hours after extraction.

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