Analyzing 16s rRNA sequences

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Effects of microalgal biomass as biofertilizer on the growth of cucumber and microbial communities in the cucumber rhizosphere

In the present study, microalgal biomass (Anabeana circinalis and Scenedesmus quadricauda) was used as biofertilizer in cucumber cultivation. The response of cucumber growth characters to microalgal biofertilizer was evaluated. Moreover, rhizosphere microbial diversity and community composition of cucumber was analyzed through 16S rRNA high-throughput Illumina sequencing data. The results showed that microalgal biomass as biofertilizer significantly promoted height, number of leaves and flower buds, and stem diameter of cucumber. The application of a high concentration of S.

12p tudichquannguyet 29-11-2021 0 0 Download

Using 16S rRNA gene as marker to detect unknown bacteria in microbial communities

Quantification and identification of microbial genomes based on next-generation sequencing data is a challenging problem in metagenomics. Although current methods have mostly focused on analyzing bacteria whose genomes have been sequenced, such analyses are, however, complicated by the presence of unknown bacteria or bacteria whose genomes have not been sequence.

7p viconnecticut2711 29-10-2020 7 0 Download

Molecular and symbiotic efficiency characterization of rhizobia nodulating bambara groundnut (Vigna subterranea L.) from agricultural soils of daloa localities in Côte D’ivoire

Bambara groundnut (Vigna subterranea L. Verdc.) is an indigenous, drought-tolerant, underutilized African food legume, with the ability to fix atmospheric N2 in symbiosis with soil rhizobial bacteria. Our work aimed to analyze the genetic diversity of 15 bacteria isolated from root nodules of Bambara groundnut coded RVC in Côte d’Ivoire and to evaluate their symbiotic characteristic in order to select the most efficient strains as biofertilizers.

13p nguaconbaynhay7 15-08-2020 7 0 Download
Analyzing 16s rRNA sequences from Vietnamese pathogenic leptospira strains and in-silico prediction of potential antigenic epitopes on Lipl21, LipL32 outer membrane lipoproteins

Leptospirosis, a zoonosis caused by Leptospira, is recognized as an emergent infectious disease. In currently, the lack of adequate diagnostic tools, vaccines are an attractive intervention strategy. In this experiment, a 550 bp fragment of large ribosomal RNA gene (16S rRNA) was sequenced and constructed phylogenetic tree from a panel of six Vietnamese pathogenic strains of Leptospira spirochetes (e.g., Pomona, Canicola, Mitis, Ictero haemohagiae, Bataviae, and Grippotyphosa).

12p nguyenxuankha_bevandan 14-08-2020 5 0 Download