Biochemical studies

This study aims to evaluate the microbial contamination level and identify bacterial colonization of the operating room in the hospital. Methods: Three sampling procedures were used in this study, which includes swabbing, air sampler and contact agar plate. Collected samples from 8 ORs were transported and microbiologically processed using standard procedures. Bacteria were identified by morphological, biochemical tests and Vitek 2 Compact system.

6p vinatisu 29-08-2024 8 1   Download

After getting pregnant by in vitro fertilization (IVF), pregnancy loss is one factor that prevents the baby from being born. This study aims to evaluate the pregnancy loss rate 12 weeks after in vitro fertilization and find some related factors.

7p vinatisu 29-08-2024 2 1   Download

Haematological and biochemical variables of blood are generally used to monitor and evaluate health, nutritional and physiological status of ruminants. The present research was conducted to study haemato-biochemical profile of goats in Vindhyan region of Uttar Pradesh, India. Total 120 blood samples were collected from 120 goats.

9p trinhthamhodang11 27-04-2021 12 2   Download

The total organic acid content in papaya was relatively low and tends to decrease from young fruit stage to ripe fruit stage. Based on this study results, Taiwanese papaya fruit should be harvested after physiological maturity and before ripening completely (from 13 weeks of age to 15 weeks of age) to ensure the nutritional value of the fruit during storage.

7p tamynhan9 02-12-2020 15 2   Download

The results of this study would partially contribute to an understanding of the differences in JAK-STAT signaling associated gene expressions between APL and other leukemia groups. This is important to apply for effective chemotherapy for each type of leukemia.

9p tamynhan6 11-09-2020 16 1   Download

The present study reports the seasonal and spatial changes in water quality of river Yamuna, India. Surface water samples were collected from three different stretches of river Yamuna i.e. Delhi, Mathura and Agra on seasonal basis from April 2014 to February 2015 and were analyzed for different water quality parameters i.e. water temperature, pH, electrical conductivity, total dissolved solids, total alkalinity, biochemical oxygen demand, chemical oxygen demand, dissolved oxygen, nitrates and phosphates...

19p kequaidan7 01-09-2020 29 1   Download

Surveying some clinical features and X-ray images of long bones, skulls, spine, blood biochemical tests and electrolytes in patients with Osteogenesis imperfecta. Evaluation of internal bone results using self-made tool to treat deformation of the lower limbs in patients with Osteogenesis imperfecta at Military Hospital 7 A.

29p angicungduoc6 21-07-2020 22 2   Download

A field experiment was conducted at Boodamanahalli village, (Bangalore rural district) to know the effect of tikka disease, caused together by Cercospora arachidicola and Cercosporidium personatum on biochemical parameters viz., total sugars, total phenols and total proteins as influenced by different sowing dates in groundnut. It included two varieties, TMV-2 a highly susceptible one and GBPD-4 a resistant with nine sowing dates starting from December 2008 to August 2009 at monthly intervals.

9p chauchaungayxua6 26-06-2020 19 2   Download

India accounts for 30% of neonatal deaths globally. Bacterial sepsis is a significant cause of morbidity and mortality in newborns. The study helps to make antibiotic policy in neonatal sepsis. The main objective is to study the incidence of multidrug resistant gram negative and gram positive organisms causing neonatal septicemia and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology over a period of one year. Sample of blood was collected under aseptic precautions and processed by standard techniques.

5p gaocaolon5 14-06-2020 24 3   Download

An experiment was carried out to study the effect of different packing and storage conditions on vase life of cut carnation cv. Kiro. The flowers packed in polypropylene at 5°C cold storage under wet condition recorded significantly highest total chlorophyll content of leaf (78.98 SPAD unit), highest total chlorophyll content of calyx (38.00 SPAD unit) and lowest ethylene estimation (0.27 ppm/h) which have extended the vase life period of carnation flower cv. Kiro.

8p cothumenhmong5 17-05-2020 20 4   Download

This study was conducted to investigate the role of silibinin in the change of biochemical indices of rabbits poisoned with of amanita virosa to provide the experimental evidence on the ability of hepatocellular protection of silibinin against toxic effect of amatoxin.

9p trieusuper 13-09-2018 39 0   Download

Trehalose metabolism is an essential component of the stress response in yeast cells. In this work we show that the products of the principal genes involved in trehalose metabolism in Schizosaccharomyces pombe, tps1+ (coding for trehalose6-P synthase, Tps1p), ntp1+ (encoding neutral trehalase, Ntp1p) and tpp1+ (that codes for trehalose-6-P phosphatase, Tpp1p), interact in vitro with each other and with themselves to form protein complexes. Disruption of the gene tps1+ blocks the activation of the neutral trehalase induced by heat shock but not by osmotic stress.

9p system191 01-06-2013 28 4   Download

The transcription factor serum response factor (SRF) regulates expression of growth factor-dependent genes and muscle-specific genes in vertebrates. Homologous factors regulate differentiation of some ectodermic tissues in invertebrates. To explore the molecular basis of these different physiological functions, the functionality of human, Drosophila melanogaster and Artemia franciscana SRFs in mammalian cells has been compared in this article. D. melanogaster and, to a lesser extend, A.

9p system191 01-06-2013 32 5   Download

In this study we describe the isolation of three genes of the CYP11B family of the guinea pig. CYP11B1 codes for the previously described 11b-hydroxylase [Bulow, H.E., Mobius, ¨ ¨ K., Bahr, V. & Bernhardt, R. (1996) Biochem. Biophys. Res. ¨ Commun. 221, 304–312] while CYP11B2 represents the aldosterone synthase gene. As no expression for CYP11B3 was detected this gene might represent a pseudogene.

9p system191 01-06-2013 38 3   Download

Starch defines a semicrystalline polymer made of two different polysaccharide fractions. The A- and B-type crystalline lattices define the distinct structures reported in cereal and tuber starches, respectively. Amylopectin, the major fraction of starch, is thought to be chiefly responsible for this semicrystalline organization while amylose is generally considered as an amorphous polymer with little or no impact on the overall crystalline organization.

11p system191 01-06-2013 45 4   Download

In a previous study, the S100A8/A9 protein, a Ca2+- and arachidonic acid-binding protein, abundant in neutrophil cytosol, was found to potentiate the activation of the redox component of the O2– generating oxidase in neutrophils, namely the membrane-bound flavocytochrome b, by the cytosolic phox proteins p67phox, p47phox and Rac ` (Doussiere J., Bouzidi F. and Vignais P.V. (2001) Biochem. Biophys. Res. Commun. 285, 1317–1320). This led us to check by immunoprecipitation and protein fractionation whether the cytosolic phox proteins could bind to S100A8/A9.

10p system191 01-06-2013 30 4   Download

The expression of mitochondrial and hydrogenosomal ADP/ATP carriers (AACs) from plants, rat and the anaerobic chytridiomycete fungus Neocallimastix spec. L2 in Escherichia coli allows a functional integration of the recombinant proteins into the bacterial cytoplasmic membrane. For AAC1 and AAC2 from rat, apparent Km values of about 40 lM for ADP, and 105 lM or 140 lM, respectively, for ATP have been determined, similar to the data reported for isolated rat mitochondria.

10p system191 01-06-2013 36 3   Download

We report here the full coding sequence of a novel mouse putative membrane-associated mucin containing three extracellular EGF-like motifs and a mucin-like domain consisting of at least 20 tandem repeats of 124–126 amino acids. Screening a cosmid and a BAC libraries allowed to isolate several genomic clones. Genomic and cDNA sequence comparisons showed that the gene consists of 25 exons and 24 introns covering a genomic region of  52 kb. The first intron is  16 kb in length and is followed by an unusually large exon ( 9.5 kb) encoding Ser/Thr-rich tandemly repeated sequences....

10p system191 01-06-2013 44 4   Download

There is extensive evidence that FcR c-chain couples to the collagen receptor glycoprotein VI (GPVI) and becomes phosphorylated on tyrosines upon receptor cross-linking. However, it is not established whether this receptor complex is sufficient to initiate the signalling cascade. We transfected GPVI and the FcR c-chain into the human erythroleukaemia cell line K562, which lacks detectable expression of GPVI and the FcR c-chain. The results show that GPVI is unable to signal when expressed alone, despite its surface expression, upon stimulation with the snake C-type lectin, convulxin....

10p system191 01-06-2013 35 3   Download

Recently, we have identified and purified minisatellite DNA binding proteins (MNBPs) that bind to the mouse hypervariable minisatellite Pc-1, from NIH3T3 cells. This study describes the isolation and characterization of a mouse leucine-rich protein (mLRP130) as one of the MNBPs that binds to the C-rich strand of Pc-1. The mLRP130 cDNA was demonstrated to encode a polypeptide of 1306 aminoacid residues with a deduced molecular mass of 137 kDa, and the mLRP130 mRNA is detected in various organs, including heart, brain, liver, skeletal muscle, kidneys and testes.

7p system191 01-06-2013 25 4   Download