Cell proliferation

The purpose of this study was to assess tracer uptake (%ID/g) in tumor-bearing mice and the relationship between uptake values and Ki-67 expression. In our model experiment, accumulation of the tracer in the tumor samples correlates with its malignancy which indicates that 18F-FLT could be a feasible PET tracer.

6p viling 11-10-2024 4 1   Download

In this study, nHAp/CS@β–CD–E nanocomposite has been synthesized by incorporating of chitosan (CS), β-cyclodextrin (β-CD), and Ficus pumila (L.) leaf extract with nano-hydroxyapatite (nHAp) via coprecipitation method. Meanwhile, the nHAp obtained from seashells as the starting material sources.

6p vikwong 29-09-2024 5 1   Download

In this study, we tested the ability of a specific culture medium supplemented with a cytokine cocktail to increase the number of total MNCs and thereby enrich CD34+UCHSCs. In addition, we investigated the effects of the bioactive peptide SL-13R on the proliferation of CD34+UC-HSCs at different culture time points and peptide doses.

7p xuanphongdacy06 18-09-2024 3 1   Download

Glioma stem cells (GSCs) are a subpopulation of stem-like cells that contribute to glioblastoma (GBM) aggressiveness, recurrence, and resistance to radiation and chemotherapy. Therapeutically targeting the GSC population may improve patient survival, but unique vulnerabilities need to be identified.

25p vimichaelfaraday 25-03-2022 24 1   Download

Cancer cells prefer utilizing aerobic glycolysis in order to exacerbate tumor mass and maintain un-regulated proliferative rates. As a key glycolytic activator, phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) has been implicated in multiple tumor type progression.

11p vielonmusk 21-01-2022 12 1   Download

The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferationassociated signaling pathway components JNK1, NF-κB and STAT3 reduced or retarded. There was stronger activation of proapoptotic proteins caspase-3, caspase-8 and BAX in the IL-1R1 KO mice at different time points (p < 0.05 or p < 0.01). IL-1R1 KO reduced inflammation and caused impaired liver regeneration after 2/3 partial hepatectomy in aged mice.

10p thiencuuchu 27-11-2021 9 1   Download

The aim of this thesis was to gain an understanding of how the simulated microgravity condition affects the development of hucMSC by investigating the changes in cell morphology, proliferation and cytoskeleton structure of hucMSCs under in vitro condition.

27p beloveinhouse01 15-08-2021 19 4   Download

Fibroblast growth factor-2 (FGF-2) is a multifunctional protein that plays an important role in the regulation of proliferation, differentiation and migration of a variety of cells. The recombinant human FGF-2 (rhFGF-2) is currently used in stem cell culture, medicine and cosmetic products. In this study, we aim to produce secreted rhFGF-2 protein from a Pichia pastoris strain containing multiple copies of the fgf-2 gene to eliminate the disadvantages of intracellular expression systems

9p larachdumlanat127 02-01-2021 13 2   Download

Xao Tam Phan (Paramignya trimera) has long been used in Viet Nam as an herbal medicine for the treatment of Hepatitis, hepatocellular carcinoma, and diabetes. This study aimed to determine the anti-proliferation effect of Paramignyatrimera extract(P.trimera extract) on HepG2 hepatocellular carcinoma cells.

6p larachdumlanat127 02-01-2021 15 1   Download

Regulators of G protein signaling (RGS) proteins modulate G protein-coupled receptor (GPCR) signaling networks by terminating signals produced by active Gα subunits. RGS17, a member of the RZ subfamily of RGS proteins, is typically only expressed in appreciable amounts in the human central nervous system, but previous works have shown that RGS17 expression is selectively upregulated in a number of malignancies, including lung, breast, prostate, and hepatocellular carcinoma.

10p caothientrangnguyen 09-05-2020 12 0   Download

The thesis aims to study the chemical composition and survey the biological activity of two species: the lizard (B. laxiflora) and the cow breast (F. hirta). - Isolate and determine the chemical structure of two species of whole lizard (B. laxiflora) and the root of cow breast (F. hirta). Evaluation of some cytotoxic activity, anti-inflammatory activity, anti-proliferation activity on acute bone marrow cell line (OCI-AML) of extracting extracts and of some isolated compounds to orient for Next application studies.

27p xacxuoc4321 11-07-2019 46 4   Download

Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate.

12p nutifooddau 27-01-2019 25 2   Download

We investigated the effect of (-)-epigallocatechin-3-gallate (EGCG) in overcoming imatinib mesylate -resistance in chronic myeloid leukaemia cells. Cell proliferation was determined by trypan blue dye exclusion test. Western blot analysis was performed to test the expression of key proteins.

9p tieuthi3006 16-03-2018 81 0   Download

Dystrobrevin is one of the intracellular components of the transmembrane dystrophin–glycoprotein complex (DGC). The functional role of this complex in normal and pathological situations has not yet been clearly established. Dystrobrevin disappears from the muscle membrane in Duchenne muscular dystrophy (DMD), which results from dystrophin mutations, as well as in limb girdle muscular dystrophies (LGMD), which results from mutations affecting other members of the DGC complex.

6p system191 01-06-2013 34 5   Download

Shikimate kinase was chosen as a convenient representative example of the subclass of a/b proteins with which to examine the mechanism of protein folding. In this paper we report on the refolding of the enzyme after denaturation in urea. As shown by the changes in secondary and tertiary structure monitored by far UV circular dichroism (CD) and fluorescence, respectively, the enzyme was fully unfolded in 4 M urea. From an analysis of the unfolding curve in terms of the two-state model, the stability of the folded state could be estimated as 17 kJÆmol)1.

9p system191 01-06-2013 46 5   Download

Liver metabolism is influenced by hormones and nutrients. Amino acids such as glutamine or leucine induce an anabolic response, which resembles that of insulin in muscle and adipose tissue. In this work, the signalling pathways and the effects of insulin were compared to those of glutamine and leucine in isolated hepatocytes from normal and streptozotocin-diabetic rats.

9p system191 01-06-2013 46 5   Download

Amplification of N-myc oncogene is a frequent event in advanced stages of human neuroblastoma and correlates with poor prognosis and enhanced neovascularization. Angiogenesis is an indispensable prerequisite for the progression and metastasis of solid malignancies, which is modulated by tumor suppressors and oncogenes. We have addressed the possibility that N-myc oncogene might regulate angiogenesis in neuroblastoma. Here, we report that experimental N-Myc overexpression results in down-regulation of leukemia inhibitory factor (LIF), a modulator of endothelial cell proliferation....

10p system191 01-06-2013 34 4   Download

In a previous study, we used the genome of serogroup B Meningococcus to identify novel vaccine candidates. One of these molecules, GNA33, is well conserved among Meningococcus B strains, other Meningococcus serogroups and Gonococcus and induces bactericidal antibodies as a result of being a mimetic antigen of the PorA epitope P1.2. GNA33 encodes a 48-kDa lipoprotein that is 34.5% identical with membrane-bound lytic transglycosylase A (MltA) from Escherichia coli. In this study, we expressed GNA33, i.e....

10p system191 01-06-2013 36 4   Download

To establish stable cell lines that produce recombinant multisubunit proteins, it is usually necessary to cotransfect cells with several independent gene constructs. Here, we show that a stepwise fusion of individually transfected cells, results in a fused cell-line that secretes a complete multisubunit protein. Functional expression of recombinant multisubunit proteins may require a defined expression ratio between each protein subunit. The cell-fusion technology described allows a predefined expression level of each subunit.

6p system191 01-06-2013 35 4   Download

Previous data showing an increase of receptor binding activity of [R16]VIP, a vasoactive intestinal peptide (VIP) structural analogue containing arginine at the position 16 of its amino acid sequence, have pointed out the importance of a positive charge at this site. Here, the functional characterization of three VIP polyaminated adducts (VIPDap, VIPSpd, and VIPSpm), obtained by a transglutaminasecatalysed reaction between the VIP Gln16 residue and 1,3-diaminopropane (Dap), spermidine (Spd), or spermine (Spm), is reported. ...

9p system191 01-06-2013 42 4   Download