Confirmation of hybridity

Confirmation of hybridity is essential to exclude selfed plants in plant breeding programmes. The morphological markers for confirmation of hybridity in chickpea are unavailable in closely related germplasm and these results in ambiguity in identification of true hybrids. In present study, cold tolerant parent ICC-16349 (donor) and cold susceptible parent GPF-2 (recipient) were crossed to generate 80 putative hybrids.

6p chauchaungayxua11 23-03-2021 11 2   Download

A putative transcription factor, named RAMY, that binds to the 20-bp O2S sequences of the regulatory region of the Amy2genepromoter has been identifiedusing the yeast one-hybrid system from a rice library. The full lengthRAMY cDNA clone encodes a 218-amino acid protein and is homologous to the late embryogenesis-abundant protein (LEA5). In vitromutagenesis and electrophoretic mobility shift assays confirmed that RAMY can bind with O2S spe-cifically throughanunusual zincfingerwithaCXCX4CX2H consensus sequence. ...

0p awards 05-04-2013 53 2   Download

A cDNA library from tobacco inoculated withRhizoctonia solaniwas con-structed, and several cDNA fragments were identified by differential hybridization screening. One cDNA clone that was dramatically repressed, NtKTI1, was confirmed as a member of the Kunitz plant proteinase inhibi-tor family. RT-PCR analysis revealed that NtKTI1was constitutively expressed throughout the whole plant and preferentially expressed in the roots and stems.

13p viettel02 19-02-2013 34 4   Download