Corresponding cDNA

Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region. Next-generation sequencing is changing the landscape of genotyping. In addition to high throughput of data, its additional advantage is that DNA templates are derived from single molecules, which is a strong merit for the phasing problem.

12p vilarryellison 29-10-2021 11 0   Download

Finding genes that are differentially expressed between conditions is an integral part of understanding the molecular basis of phenotypic variation. In the past decades, DNA microarrays have been used extensively to quantify the abundance of mRNA corresponding to different genes, and more recently high-throughput sequencing of cDNA (RNA-seq) has emerged as a powerful competitor.

18p viwyoming2711 16-12-2020 17 1   Download

The yeast Pichia pastoris expression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12–475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector.

8p nutifooddau 27-01-2019 26 2   Download

In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide.

18p nutifooddau 27-01-2019 31 1   Download

The plastid transcription kinase (PTK), a component of the major RNA polymerase complex from mustard chloroplasts, has been implicated in redox-mediated regulation of plastid gene expression. A cloning strategy to define the PTK gene(s) resulted in the isolation of a full-length cDNA for a protein with overall high homology with the a subunit of cytosolic casein kinase (CK2) that contained an N-terminal extension for a putative plastid transit peptide.

9p system191 01-06-2013 44 3   Download

Murine pregnancy-associated plasma protein-A (PAPP-A) cDNA encoding a 1545 amino-acid protein has been cloned. We have also identified and cloned cDNA that encodes a novel variant of PAPP-A, PAPP-Ai, carrying a 29-residue highly basic insert. The point of insertion corresponds to a junction between two exons in the human PAPP-A gene. The human intron flanked by these exons does not encode a homologous corresponding insert, which is unique to the mouse.

10p research12 01-06-2013 37 4   Download

Dj2 is a member of the DnaJ family of proteins, which regulate the chaperoning functionof the hsp70s.We isolated a monkey cDNA dj2 clone corresponding to the large mRNA species encoded by the gene. This mRNA differs from the small mRNAproduced by the same gene in that it contains a long 3¢ untranslated region. Both messages were found to be equally stable and to produce the same protein, which is susceptible to farnesylation.

8p research12 29-04-2013 30 2   Download

Pycnoporus cinnabarinuslaccaselac1genewasoverexpressed in Aspergillus niger, a well-known fungal host producing a large amount of homologous or heterologous enzymes for industrial applications. The corresponding cDNA was placed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter as a strong and constitutive pro-moter. The laccase signal peptide or the glucoamylase preprosequence ofA.

8p research12 23-04-2013 44 2   Download

BLASTanalysis of the human and mouse genome sequence databases using the sequence of the human CMP-sialic acid:b-galactoside a-2,6-sialyltransferase cDNA (hST6Gal I, EC2.4.99.1) as a probe allowed us to identify a putative sialyltransferase gene on chromosome 2. The sequence of the corresponding cDNA was also found as an expressed sequence tag of human brain.

12p tumor12 20-04-2013 24 1   Download

Pest insects such asHelicoverpaspp. frequently feed on plants expressing protease inhibitors. Apparently, their digestive system can adapt to the presence of protease inhibitors. To study this, a trypsin enzymewas purified from the gut of insects that were raised on an inhibitor-containing diet. The amino-acid sequence of this enzyme was analysed by tandem MS, which allowed assignment of 66% of the mature protein amino acid sequence. This trypsin, called HzTrypsin-S, corresponded to a known cDNA sequence fromHelicoverpa. ...

10p tumor12 20-04-2013 23 2   Download

Glucose transporters (GLUTs) have been implicated in adaptive and survival responses to hypoxic stress in mam-mals. In fish, the expression and regulation ofGLUT in relation to hypoxia remains unexplored. Here we describe the identification ofa hypoxia-responsive glucose transpor-ter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans
11 kb ofgenomic sequence and consists of12 exons and 11 introns, and an open reading frame (ORF) of 1599 bp encoding a poly-peptide of533 amino acids, with a predictedmolecularmass of
57 kDa and a pI of8.34....

8p fptmusic 16-04-2013 37 1   Download

We previously identified a partial expressed sequence tag clone corresponding toNARG2in a screen for genes that are expressed in developing neurons and misexpressed in transgenic mice that lack functional N-methyl-D-aspartate receptors. Here we report the first characterization of the mouse and humanNARG2genes, cDNAs and the proteins that they encode.Mouse andhumanNARG2 consist of 988 and 982 amino acids, respectively, and share 74% identity.

9p awards 05-04-2013 37 1   Download

Malate dehydrogenase (MDH) may be important in car-bohydrate and energymetabolism inmalarial parasites. The cDNA corresponding to the MDHgene, identified on chromosome 6 of thePlasmodium falciparumgenome, was amplified by RT-PCR, cloned and overexpressed in Escherichia coli. The recombinant PfMDH was purified to homogeneity and biochemically characterized as an NAD + (H)-specific MDH, which catalysed reversible inter-conversion of malate to oxaloacetate.

15p awards 05-04-2013 76 6   Download

RNA polymerase II (RNAPII) requires a set of general transcription factors )TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH)to initiate transcription from a gene promoterin vitro. General transcription factors have been isolated from Saccharomyces cerevisiae,rat, human and Drosophila,and their corresponding cDNAs have been cloned. In this report, we describe a reconstitutedin vitro transcription system that consists of the following prepara-tions of factors from the yeastSchizosaccharomyces pombe: affinity-purified RNAPII, TFIIH, and recombinant TBP, TFIIB, TFIIE and TFIIF. ...

12p dell39 03-04-2013 57 3   Download

The rapid increase in the number of putative cDNA sequences encoding crustacean hyperglycemic hormone (CHH) family in various tissues [either from the eyestalk (ES) or elsewhere] underscores a need to identify the corresponding neuropeptides in relevant tissues.

12p media19 05-03-2013 28 2   Download

A cDNA corresponding to theAt3g26690 3 gene, which encodes a Nudix protein (AtNUDT13) with predicted mitochondrial localization, was iso-lated from an Arabidopsis thalianalibrary. The 202 amino acid AtNUDT13 polypeptide was overexpressed inEscherichia coliand purified to homo-geneity.

9p media19 04-03-2013 19 1   Download