Kinetics of inactivation

Synergistic combination of heat and ultrasonic waves under pressure for Cronobacter sakazakii inactivation in apple juice has many contents: Microorganism, growth conditions, and treatment media, MS/MTS treatments, Heat treatments, Incubation of treated samples and colony counting, Kinetics of inactivation, Occurrence of sublethal damages after heat and MTStreatments in apple juice and counts evolution during storage underrefrigeration,...

7p cscpubka 05-05-2014 109 5   Download

The involvement of the lysine residue present at the active site of Ehrlich ascites carcinoma (EAC) cell glyceraldehyde-3-phosphate dehydrogenase (Gra3P DH) was investigated by using the lysine specific reagents trinitrobenzenesulfonic acid (TNBS) and pyridoxal phosphate (PP). Both TNBS and PP inactivated EAC cell Gra3P DH with pseudo-first-order kinetics with the rate dependent on modifier concentration. Kinetic analysis, including a Tsou plot, indicated that both TNBS and PP apparently react with one lysine residue per enzyme molecule.

8p system191 01-06-2013 37 3   Download

The effects of divalent metal cations on structural thermostability and the inactivation kinetics of homologous class II d-xylose isomerases (XI; EC 5.3.1.5) from mesophilic (Escherichia coliand Bacillus licheniformis), thermophilic (Thermoanaerobacterium thermosulfurigenes), and hyperther-mophilic (Thermotoga neapolitana) bacteria were examined.

0p awards 06-04-2013 34 2   Download

Incubation of an NAD + -dependent succinic semialdehyde dehydrogenase from bovine brain with 4-dimethylamino-azobenzene-4-iodoacetamide (DABIA) resulted in a time-dependent loss of enzymatic activity. This inactivation followed pseudofirst-order kinetics with a second-order rate constant of 168M )1 Æmin )1 .The spectrumofDABIA-labeled enzyme showed a characteristic peak of the DABIA alkyl-ated sulfhydryl group chromophore at 436 nm, which was absent from the spectrum of the native enzyme.

0p awards 05-04-2013 31 2   Download

Inactivation of factor Va (FVa) by activated protein C (APC) is a predominant mechanism in the down-regulation of thrombin generation. In normal FVa, APC-mediated inactivation occurs after cleavage at Arg306 (with corres-pondingrate constantk¢306) or after cleavage at Arg506 (k506 ) and subsequent cleavage at Arg306 (k306). We have studied the influence of heparin on APC-catalyzed FVa inactivation by kinetic analysis of the time courses of inac-tivation. Peptide bond cleavage was identified by Western blottingusingFV-specific antibodies. ...

13p dell39 03-04-2013 41 4   Download

A complex of chagasin, a protein inhibitor fromTrypanosoma cruzi, and papain, a classic family C1 cysteine protease, has been crystallized. Kinetic studies revealed that inactivation of papain by chagasin is very fast (kon= 1.5·10 6 m )1 Æs )1 ), and results in the formation of a very tight, reversible complex (Ki =36pm), with similar or better rate and equilib-rium constants than those for cathepsins L and B.

14p vinaphone15 27-02-2013 52 2   Download