Murashige and Skoog

Nghiên cứu đã xác định được môi trường Murashige and Skoog (MS) đặc bổ sung 0,5 mg/l BA là thích hợp nhất để nhân chồi Dạ yến thảo hoa tím, với hệ số nhân chồi đạt 19,78 chồi/mẫu, chiều cao chồi đạt 2,36cm sau 6 tuần nuôi cấy.

9p vigojek 02-02-2024 4 1   Download

This study investigated the effects of different PGRs on in vitro plant regeneration of Anthurium scherzerianum Schott and acclimatization in the ex vitro stage. Depending on the objective of each experiment, the in vitro shoots were induced from callus cultured on a modified Murashige and Skoog (MS) medium containing cytokinins and auxins.

11p vicaptainmarvel 21-04-2023 3 2   Download

An efficient regeneration protocol was established for a rare plant species, Begonia coptidifolia. Using leaf explants, adventitious shoots were induced on Murashige and Skoog (MS) basal medium using various concentrations (1.0–10.0 mM) of three cytokinins, 6-benzyladenine (BA), thidiazuron (TDZ) and kinetin (KIN). However, adventitious shoots could not be induced on MS medium supplemented with auxins [α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)] tested in the 1.0–10.0 mM range.

8p hanthienngao 30-11-2021 11 1   Download

To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid).

11p hanthienngao 30-11-2021 9 1   Download

Nghiên cứu nhân giống chuối sáp bằng phương pháp nuôi cấy mô nhằm cung cấp nguồn cây con ổn định di truyền và sạch bệnh cho sản xuất. Kết quả nghiên cứu cho thấy, môi trường thích hợp nhất cho giai đoạn tái sinh chồi chuối sáp là môi trường Murashige and Skoog (MS) có bổ sung 5 mg/L benzyl aminopurine (BA). Môi trường MS bổ sung 5 mg/L BA và 2 ml/L dịch thủy phân cá cho hiệu quả nhân chồi cao đạt 5,9 chồi/mẫu, chiều cao chồi đạt 3,3 cm và số lá trung bình 2,5 lá sau 35 ngày nuôi cấy.

5p parasite 02-06-2021 36 7   Download

The micropropagation protocol of tropical, small evergreen, true mangrove, and woody shrub or tree species, Aegiceras corniculatum, has been standardized. Axillary shoot proliferation was induced in vitro from nodal explants excised from 3-4 years field-grown old plant which was then used as explants for the establishment of in vitro cultures. Surface-sterilized explants were cultured on the Murashige and Skoog (MS) basal medium supplemented with different concentrations and a combination of growth regulators.

7p caygaocaolon11 21-04-2021 4 1   Download

The present study demonstrates a simple and efficient method for callus induction of Bamboo. The leaf, node and seed are used as explants for induction of callus. These explants were subjected to sterilization treatment and it is found that Tween -20, bavistin and ethanol are sufficient for proper sterilization of explants. Then these explants were subjected to various concentrations of growth hormone 2,4-Dichlorophenoxyacetic acid (2,4-D) added in Murashige and Skoog Media. It was concluded that the seeds are suitable explants for rapid induction of callus.

10p chauchaungayxua10 18-03-2021 7 1   Download

The research was conducted in commercial tissue culture laboratory, IGKV, Raipur, C.G. from the period of March 2015 to August 2017. Present investigation was conducted to assess the suitable explant for callusing using different treatment of auxin and cytokinin (NAA and BAP) in semi-solid Murashige and Skoog (MS) media.

6p cothumenhmong9 18-01-2021 9 1   Download

In this study, we investigated four factors to improve the hairy root induction in Urena lobata L. These factors include: age of plant (15-day-old in vitro plants, 45-day-old in vitro plants and after two subculture generations plants), different parts of plant (roots, stems, and leaves), infection time (10, 20 and 30 minutes), and culture medium (Murashige and Skoog (MS), Gamborg B5 medium (GB5) and Woody plant medium (WPM)).

8p larachdumlanat127 02-01-2021 5 2   Download

Different nutrient solutions alone or in combinations were applied as foliar spray on Asiatic lilium hybrid Tresor and evaluated for different characters. Plants sprayed with only water were taken as control. Other treatments were done with the dose of nutrients used in preparation of tissue culture Murashige and Skoog (MS) medium (1962) in different combinations.

7p caygaocaolon8 07-11-2020 15 2   Download

Nghiên cứu nhân giống in vitro lan Bạch hạc nhằm bảo tồn và phát triển giống lan quý này. Kết quả cho thấy protocorm-like body (PLB) hình thành từ nuôi cấy đỉnh sinh trưởng trên môi trường ½ Murashige and Skoog (1962, ½ MS) bổ sung NAA sau 42 ngày nuôi cấy.

8p vioklahoma2711 18-11-2020 46 2   Download

This study evaluated the effect of particular phytohormones and a plant growth promoting rhizobacteria on the growth and viability of Epithelantha micromeris callus culture. E. micromeris callus growth without the inoculant, diminished in Murashige-Skoog (MS) + naphthalene acetic acid (NAA) medium (34.78%) and MS + indole acetic acid (IAA) (26.08%) experiments.

8p chauchaungayxua8 03-10-2020 15 1   Download

Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The aim of this study was to set up a protocol for large scale for in vitro propagation of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1.0 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number.

13p trinhthamhodang7 31-08-2020 9 1   Download

Five maize lines prevalently used by breeders in the maize program were screened for their ability to form type II callus. Mature seeds were used as explant for in vitro culturing. One inbred line showed high embryogenic response and formed friable, type II calli and also exhibited successful plant regeneration. Ten culture media compositions based on Murashige and Skoog (1962) salts thus investigated differed with respect to the combinations of concentrations of auxins (IBA, NAA, picloram, 2, 4-D) and cytokinins (BAP, kinetin) were used.

9p angicungduoc5 14-06-2020 4 0   Download

Banana is one of the important fruit crops and food source for million people in developing countries. In the study explants (Suckers) of two banana cultivars were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and Kinetin sole and in combination for shoot initiation and multiplication.

10p trinhthamhodang5 16-05-2020 10 0   Download

Liquid Murashige and Skoog (MS) medium in varying strengths, is the most commonly utilized medium for proficient rooting in the regenerated plants in doubled haploidy breeding protocols. On the other side, CPV (cocopeat: perlite: vermiculite) medium is currently being used in hardening of tissue culture regenerated plants.

9p angicungduoc4 26-04-2020 15 1   Download

The present investigation was carried out at Tissue Culture Laboratory of S.K.N. Agriculture University, Jobner, India during 2018-19. Experiment was laid out using completely randomized design (CRD) with ten replications. Six culture media viz. Murashige and Skoog media, Nitsch & Nitsch media, woody plant medium, Schenk and Hildebrant medium, White’s medium and Khudson solution– C were tested for direct shoot proliferation and callus induction at most responsive level of plant growth regulators in Bael (Aegle marmelos L.).

6p caygaocaolon4 04-04-2020 12 1   Download

An in vitro protocol for plant regeneration through organogenesis was established for Picrorhiza kurroa Royle Ex. Benth (Scrophulairaceae). The plant is a principle source of glycoside that is Picroside-I, Picroside-II and Kutkoside. Callus was induced on Murashige and Skoog basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA) using leaf, petiole and stem explants. Shoots were regenerated on MS media with BA (1.5 to 2.5 mgl-1 ) combined with auxin (IBA or NAA) in the range of 0.5 to 2.5 mgl-1 . Rooting was initiated with full or half media supplemented with 0.

7p cothumenhmong3 22-02-2020 14 1   Download

Gladiolus (Gladiolus hybrida) is an important ornamental plant cultivated world over for its attractive spikes. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. In vitro propagation techniques, is superior to conventional propagation and produces disease free plants in huge quantities. In vitro regeneration of gladiolus cultivars, Sylvia, White Prosperity and Amsterdam was achieved using shoot bud of cormels as an explant. Sylvia variety recorded the best for callus induction and regeneration than other two varieties.

6p trinhthamhodang3 14-02-2020 18 0   Download

Arbuscular mycorrhizal (AM) fungi are obligate symbiont and integrate with most of terrestrial plants. AMF belongs to the phylum Glomeromycota that cannot complete their life cycle without establishing a functional symbiosis with host plant. In the present investigation, Glomus intraradices Spores were surface sterilized and transferred to different media viz., one per cent water medium agar medium, Murashige and Skoog medium. Modified Strulla Romand (MSR) medium and white’s medium with root exudates of Agrobacterium rhizogenes MTCC-532 transformed hairy roots of chickpea.

10p nguaconbaynhay3 07-02-2020 8 1   Download