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The thioredoxin system, found in all living creatures, consists of thioredoxin protein (Trx), thioredoxin reductase enzyme (TrxR), and NADPH. In this study, turkey liver mitochondrial TrxR enzyme with 3.07 EU × mg −1 specific activity was purified 990-fold in a yield of 2.05% using 2’,5’-ADP Sepharose 4B affinity chromatography. The purity of the enzyme was measured and the molecular weight of its subunits was determined to be 45.5 kDa by SDSPAGE. The molecular mass of the enzyme’s natural state was found to be 88 kDa using Sephadex-G 150 gel filtration chromatography.
13p
langthannam
29-12-2021
10
0
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Microbial diversity is the main key point during the studies of microbial ecology. The diversity of edible fungal species in an area affects the nutritional behavior of that community.
12p
trinhthamhodang5
16-05-2020
9
1
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An extensive survey was conducted in different District (Upper Siang, West Siang and East Siang) of Arunachal Pradesh during 2015-17 to study the genetic diversity of carambola and to identify superior genotypes having sweet in taste based on the descriptor of NBPGR carambola.
10p
kethamoi4
16-04-2020
12
1
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Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) in humans. The identification of specific antigens of T. canis is important in order to develop better diagnostic techniques. The excretory-secretory larval antigens of T. canis (ESLA) were prepared by in vitro culturing of T. canis larvae in RPMI 1640 medium. These antigens were separated using sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDSPAGE) which revealed 9 protein bands at a molecular weight of 17, 18, 22, 24, 26, 28, 30, 32 and 120 kDa.
8p
quenchua2
18-12-2019
8
0
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Nghiên cứu này áp dụng phương pháp đo chỉ số hấp thụ quang học UV-Vis và điện di biến tính SDSPAGE để đánh giá chất lượng phycocyanin. Kết quả cho thấy, tinh sạch phycocyanin từ tảo tươi theo 2 bước (tủa bằng ammonium sulfate rồi chạy sắc ký qua cột LH20) cho phycocyanin có độ tinh sạch A620/280 là 1,2, phù hợp cho ngành thực phẩm và hóa mỹ phẩm. Đánh giá hàm lượng phycocyanin trong sản phẩm tảo thương mại cho thấy, sản phẩm của hãng Vinataor trong nước có hàm lượng phycocyanin cao hơn một số sản phẩm thương mại nhập ngoại được đánh giá.
5p
trangcham1896
21-12-2018
97
1
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