Wharton’s jelly-derived mesenchymal stem cells are highly homogeneous cells population, easily expanded, especially they can bring potential applications. However, their yield depends on the method of cells extraction conducted. Our research includes two purposes: 1/Procedure for isolating and differentiating mesenchymal stem cells from Wharton’s jelly, 2/Determining the main features of mesenchymal stem cells isolated from Wharton’s jelly.

That is cause we conducted this thesis, which has two subjects as follows: Describing some clinical and para-clinical characteristics and histology of chronic wound. Evaluating the effective of autologous adipose tissue derived stem cell transplation in chronic wound treatment.