Stem cells

Wharton’s jelly-derived mesenchymal stem cells are highly homogeneous cells population, easily expanded, especially they can bring potential applications. However, their yield depends on the method of cells extraction conducted. Our research includes two purposes: 1/Procedure for isolating and differentiating mesenchymal stem cells from Wharton’s jelly, 2/Determining the main features of mesenchymal stem cells isolated from Wharton’s jelly.

7p vihatake 06-01-2025 0 0   Download

The aim of this thesis was to gain an understanding of how the simulated microgravity condition affects the development of hucMSC by investigating the changes in cell morphology, proliferation and cytoskeleton structure of hucMSCs under in vitro condition.

27p beloveinhouse01 15-08-2021 19 4   Download

That is cause we conducted this thesis, which has two subjects as follows: Describing some clinical and para-clinical characteristics and histology of chronic wound. Evaluating the effective of autologous adipose tissue derived stem cell transplation in chronic wound treatment.

23p petsematary 02-06-2021 17 3   Download

Isolation, identification and culture successfully mesenchymal stem cell sample from rabbit bone marrow. Differentation mesenchymal stem cell towards the osteoblast and cryopreservation cell after differentiation.

12p bibianh 25-09-2019 41 2   Download

Tế bào gốc tạo máu (HSC: Hematopoietic Stem Cell) đã được nghiên cứu hơn nửa thế kỷ nay và là một trong những lĩnh vực tiến bộ và nổi bật nhất trong y sinh học hiện đại.

37p haokhitihon 21-03-2014 266 46   Download

Haematopoiesis, the differentiation of haematopoietic stem cells and progenitors into various lineages, involves complex interactions of transcription factors that modulate the expression of downstream genes and mediate proliferation and differentiation signals. Commitment of pluripotent haematopoietic stem cells to the erythroid lineage induces erythropoiesis, the production of red blood cells. This process involves a concerted progression through an erythroid burst forming unit (BFU-E), an erythroid colony forming unit (CFU-E), proerythroblast and an erythroblast....

12p research12 01-06-2013 49 4   Download

Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, ¯uorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stembromelain lacks awell de®ned tertiary structure as seen by ¯uorescence and near-UVCDspectra.Far-UVCDspectra showretentionof some native like secondary structure at pH 2.0. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India Equilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.

6p research12 29-04-2013 32 3   Download

Human hepatitis delta (HDV) ribozyme is one of small ribozymes, such as hammerhead and hairpin ribozymes, etc. Its secondary structure shows pseudoknot structure com-posed of four stems (I to IV) and three single-stranded regions (SSrA, -B and -C). The 3D structure of 3¢-cleaved product of genomic HDV ribozyme provided extensive information about tertiary hydrogen bonding interactions between nucleotide bases, phosphate oxygens and 2¢OHs including new stem structure P1.1.

12p tumor12 22-04-2013 38 3   Download

We report on a sequence-specific double-stranded DNA labelling strategy in which a stem–loop triplex forming oligonucleotide (TFO) is able to encircle its DNA target. Ligation of this TFO to either a short hairpin oligonucleotide or a long double-stranded DNA fragment leads to the for-mation of a topological complex. This process requires the hybridization of both extremities of the TFO to each other on a few base pairs. The effects of different factors on the formation of these complexes have been investi-gated. ...

10p fptmusic 12-04-2013 32 2   Download

Eukaryotic chromosomal DNA is densely packaged in the nucleus and organized into discrete domains of active and inactive chromatin. Gene loci that are activated during the process of cell differentiation undergo changes that result in modifications of specific histone tail residues and in loosening of chromatin structure. Theb-globin genes are expressed exclusively in eryth-roid cells.

10p dell39 27-03-2013 50 6   Download

Nanog is a transcription factor that plays key roles in the self-renewal and maintenance of pluripotency in human embryonic stem (ES) cells. Among Nanog’s 11 pseudogenes, NANOGP8 theoretically could be a retrogene, but was considered unlikely as it has not been identified in any expressed sequence tags (ESTs).

8p inspiron33 26-03-2013 51 5   Download

Bone morphogenetic protein (BMP) signals play key roles throughout embryology, from the earliest patterning events, via tissue specification, through organ development and again in germ cell differentiation. While both input and the transducer molecules are rather well studied, the final outcome of a BMP signal is basically unpredictable and differs enormously between previously studied cell types.

9p galaxyss3 19-03-2013 59 4   Download

We recently reported that intermittent hypoxia facilitated the proliferation of neural stem⁄progenitor cells (NPCs) in the subventricule zone and hip-pocampusin vivo. Here, we demonstrate that hypoxia promoted the prolif-eration of NPCsin vitro and that hypoxia-inducible factor (HIF)-1a, which is one of the key molecules in the response to hypoxia, was critical in this process.

11p media19 06-03-2013 40 1   Download

During mammalian development, it is essential that the proper epigenetic state is established across the entire genome in each differentiated cell. To date, little is known about the mechanism for establishing epigenetic modi-fications of individual genes during the course of cellular differentiation.

7p media19 06-03-2013 35 3   Download

The specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). To identify the genes responsible for hepatic differentiation, we examined the gene expres-sion profiles of AT-MSC-derived hepatocytes (AT-MSC-Hepa) using several microarray methods.

14p media19 06-03-2013 42 1   Download

A dominant negative form of p63,DNp63a, is critical for maintaining the proliferative potential of epidermal stem cells and progenitor cells. The expression ofDNp63aalso confers a selective advantage for cancer cell survival, underscoring the importance of DNp63a in both normal and neoplastic stratified epithelia.

12p media19 05-03-2013 24 1   Download

Activating enhancer-binding protein (AP)-2ewas previously described as a new regulator of integrina10 expression in cartilage. In this study, we ana-lyzed the expression of AP-2ein differentiated chondrocytes and in human mesenchymal stem cells (HMSCs), which have been differentiated into chondrocytes in vitro.

11p viettel02 22-02-2013 27 3   Download

MicroRNAs (miRNAs) are noncoding regulatory RNAs that function via the degradation of target mRNAs and inhibition of translation. They are found widely in higher eukaryotic organisms, and in several species that have been closely examined, hundreds of miRNAs have thus far been discovered with mechanistically conserved, yet functionally diverse, roles necessary for the proper development, homeostasis and metabolism of the organisms.

10p viettel02 22-02-2013 44 3   Download

Polypyrimidine tract-binding protein (PTB) is a widely expressed RNA-binding protein with multiple roles in RNA processing, including the splic-ing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site-dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear.

11p viettel02 20-02-2013 35 3   Download

A cDNA library from tobacco inoculated withRhizoctonia solaniwas con-structed, and several cDNA fragments were identified by differential hybridization screening. One cDNA clone that was dramatically repressed, NtKTI1, was confirmed as a member of the Kunitz plant proteinase inhibi-tor family. RT-PCR analysis revealed that NtKTI1was constitutively expressed throughout the whole plant and preferentially expressed in the roots and stems.

13p viettel02 19-02-2013 34 4   Download