Synthase triterpene

The pentacyclic triterpenes, in particular ursolic acid and oleanolic acid and their derivatives, exist abundantly in the plant kingdom, where they are well known for their anti-inflammatory, antitumour and antimicrobial properties.

15p cosis54 05-01-2013 18 3   Download

Using a degenerate primer designed from triterpene synthase sequences, we have isolated a new gene from the medicinal plantArtemisia annua. The predicted protein is highly similar to b-amyrin synthases (EC 5.4.99.–), sharing amino acid sequence identities of up to 86%.

8p media19 06-03-2013 12 2   Download

An oxidosqualene cyclase cDNA, LcIMS1, was isolated from cultured cells of Luffa cylindrica Roem. by heterologous hybridization with cDNA of Glycyrrhiza glabra b-amyrin synthase. Expression of LcIMS1 in yeast lacking endogenous oxidosqualene cyclase activity resulted in the accumulation of isomultiflorenol, a triterpene. This is consistent with LcIMS1 encoding isomultiflorenol synthase, an oxidosqualene cyclase involved in bryonolic acid biosynthesis in cultured Luffa cells.

7p system191 01-06-2013 15 2   Download

Oleanane-type triterpene is one of the most widespread triterpenes found in plants, together with the lupane type, and these two types often occur together in the same plant. Bruguiera gymnorrhiza(L.) Lamk. and Rhizo-phora stylosaGriff. (Rhizophoraceae) are known to produce both types of triterpenes.

15p media19 04-03-2013 19 1   Download