Using monoclonal antibodies

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies

9p hoami_266 16-09-2011 30 2   Download

Three tests were carried out where each volume anti A monoclonal antibody was mixed with one, two and three volume anti-B monoclonal antibody separately. And the best results were obtained when one volume anti A monoclonal antibody was mixed with one volume anti-B monoclonal antibody. The corresponding titers of the anti-A,B antibody were 1/128 for red blood group A and 1/128 for red blood group B. The intensity of agglutination reached 3+ for both A and B red blood groups.

7p cumeo2009 30-07-2018 7 0   Download

Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21st and 22nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels.

7p vitamix 19-02-2019 2 0   Download

We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine.

9p caplock2711 19-02-2019 3 0   Download

Twenty-five years ago, Georges Köhler and César Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics (1). However, in spite of their early promise, monoclonal antibodies (MAbs) were largely unsuccessful as therapeutic reagents resulting from insufficient activation of human effector functions and immune reactions against proteins of murine origin.

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Recombinant human transcobalamin (TC) was probed with 17 monoclonal antibodies (mAbs), using surface plasmon resonance measurements. These experiments identified five distinct epitope clusters on the surface of holo-TC. Western blot analysis of the CNBr cleavage fragments of TC allowed us to distribute the epitopes between two regions, which spanned either the second quarter of the TC sequence GQLA…TAAM(103–198) or the C-ter-minal peptide LEPA…

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Although serum is a main source of essential nutrients and growth factors, its use creates highly complex and poorly defined media. The use of serum-free media is more efficient than the use of media containing serum especially when the purification and characterization of antibodies are considered.

8p caplock2711 19-02-2019 3 0   Download

Trading partner Canada has established the Canadian Adaptation and Rural Development (CARD), national and regional adaptation programs that provide assistance to the sector in the area of research, innovation, capturing market opportunities, environmental sustainability, food safety and quality, human resource capacity building and rural development.

60p tieuyeuquai456 08-10-2012 44 6   Download

A Gallup study of over 250,000 sales people and 25,000 sales managers supports our internal findings that the highest performers use a variety of sales approaches and styles. Even within the same company, many different sales approaches are used by these top performers. In fact, if all the top performers were forced to use a single sales style, many of them would cease to be top producers, would get frustrated and quit. Gallup’s conclusion: No one style is best. Gallup’s study supports our consistent findings: The bank must be flexible enough to allow people to...

0p khanhchilam 01-04-2013 33 5   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISA

8p dauphong3 26-12-2011 35 4   Download

In 1930 Ferguson modified the technique and published his series of 280 patients who had prostate needle aspiration biopsy using an 18-gauge needle via the transperineal approach and was able to remove adequate tissue in 78 to 86% of his cases (Ferguson, 1930) (figure 6). The patient was placed in the lithotomy position and local anaesthetic was infiltrated just lateral to the median raphe 1cm anterior to the anus. The index finger of the left hand was introduced into the rectum guiding an 18 gauge needle introduced into the perineum taking care to avoid the rectum and urethra....

546p ti_du_hoang 10-08-2012 38 4   Download

Historically, the Ontario Medical Association (OMA) has taken a strong stand advocating reductions in air pollution in Ontario as an essential means to improve public health. Beginning in 1967, the Ontario Medical Association Council endorsed a program of air pollution control in Ontario which included the establishment of monitoring stations and regulation of emissions from industry and vehicular traffic (15). Shortly thereafter, the government enacted the Air Pollution Control Act encompassing a number of the recommendations from the OMA.

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There are several exemptions in many State laws that leave children unprotected in vehicles. 8 In nearly half of the States, children can ride unsecured if all safety belts are in use. This means that if a vehicle is overcrowded and other occupants are using all of the safety belts, nobody can be cited if children are unbelted. Another common exemption allows parents, guardians, or other adults to “attend to the personal needs of the child.” This exemption permits an adult to carry a child on his or her lap, while the vehicle is in motion, for...

117p nhacchovina 23-03-2013 24 4   Download

Finally, the last design goal for the API is that it should be possible for a library to use the mechanism without fear of conflicts with the main program. This allows 3 party code that uses the API to be linked into the application without conflict. While on the surface this appears to be obvious, several counter examples exist. Within a process, a signal may only have a single sig- nal handler registered, so library code typically can not use signals. X-window applications only allow for a sin- gle event loop.

0p nhacsihuytuan 06-04-2013 26 3   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)

7p hoami_266 15-09-2011 34 2   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evaluation of anti-IL-6 monoclonal antibody therapy using murine type II collagen-induced arthritis...

12p thulanh27 13-12-2011 38 2   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Localization of antigenic sites at the amino-terminus of rinderpest virus N protein using deleted N mutants and monoclonal antibody

7p hoami_266 16-09-2011 38 1   Download

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

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Neuropilin (NRP) and plexin (Plex) that are now known to be semaphorin receptors were initially identified as antigens for monoclonal antibodies (MAbs) that bound to particular neuropiles and plexiform layers of the Xenopus tadpole optic tectum, several years before the discovery of semaphorin. The extracellular segment of the NRP protein is a mosaic of 3 functionally different protein motifs that are thought to be involved in molecular and/or cellular interactions, suggesting that NRP serves in a various cell-cell interaction by binding a variety of molecules.

156p hotmoingay 03-01-2013 27 1   Download

In addition to explicitly named Properties, “code” and “id” attributes are special cases. Every entity in the Thésaurus – Concepts, Kinds, Properties, and Roles – has an associated “code” and “id.” These are used as unique identifiers in the Apelon development software, and, as such, are not defined explicitly as Roles or Properties. This required a hard-coded definition of both entities. They were defined as owl:AnnotationProperties, just like other Properties in the Thésaurus, and this allowed them to be attached to all of the definitions in the ontology.

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