Variceal bleeding is a severe complicaton of portal hypertension due to cirrhosis with high rate of motality. The aims of this study was to compare the accuracy of CTP - crea (creatinine-modified Child Turcotte Pugh score) with traditional CTP and MELD score for predicting in rebleeding and mortality within first five days and 6-week in cirrhotic patients with acute variceal bleeding.

Bài viết trình bày thang điểm Child Turcotte Pugh (CTP) phối hợp với creatinine máu giúp nâng cao giá trị tiên lượng bệnh nhân xơ gan xuất huyết do vỡ giãn tĩnh mạch đã được một số nghiên cứu trên thế giới đề xuất áp dụng. Nghiên cứu này nhằm áp dụng thang điểm CTP - Creatinine trong tiên lượng tái xuất huyết, tử vong sớm và tái xuất huyết, tử vong trong vòng 6 tuần ở bệnh nhân xơ gan nhập viện tại khu vực miền Trung, Việt Nam.

Báo cáo Asignment Com2042 trình bày về cách cài đặt windows Server 2008 và windows 7/Windows XP, cấu hình CTP/IP, cài đặt Domain controller Additional Domain controller, Join các máy tính vào Domain, triển khai tạo các đối tượng OU, Groups, Users tương ứng của mỗi phòng ban và một số nội dung khác.

CTP synthase catalyzes the reaction glutamine + UTP +ATPfiglutamate + CTP + ADP + Pi.The rate of the reaction is greatly enhanced by the allosteric activator GTP.We have studied the glutaminase half-reactionofCTP synthase from Lactococcus lactisand its response to the allosteric activator GTP and nucleotides that bind to the active site. In contrast to what has been found for the Escherichia colienzyme, GTP activation of the L. lactis enzyme did not result in similarkcat values for the glutami-nase activity and glutamine hydrolysis coupled to CTP synthesis. ...

Cytidine 5¢-triphosphate synthase catalyses the ATP-dependent formation of CTP from UTP using either ammonia orL-glutamine as the source of nitrogen. When glutamine is the substrate, GTP is required as an allosteric effector to promote catalysis. Limited trypsin-catalysed proteolysis, Edman degradation, and site-directed muta-genesis were used to identify peptide bonds C-terminal to three basic residues (Lys187, Arg429, and Lys432) of Escherichia coliCTP synthase thatwere highly susceptible to proteolysis. ...

Theuppgene, encoding uracil phosphoribosyltransferase (UPRTase) from the thermoacidophilic archaeon Sulfolobus solfataricus, was cloned and expressed inEscherichia coli. The enzyme was purified to homogeneity. It behaved as a tetramer in solution and showed optimal activity at pH 5.5 when assayed at 60
C. Enzyme activity was strongly stimulated by GTP and inhibited by CTP.

GTP is an allosteric activator of CTP synthase and acts to increase thekcat for the glutamine-dependent CTP synthesis reaction. GTP is suggested, in part, to optimally orient the oxy-anion hole for hydrolysis of glutamine that takes place in the glutamine amidotransferase class I (GATase) domain of CTP synthase. In the GATase domain of the recently published structures of theEscherichia coliand Thermus thermophilusCTP synthases a loop region immediately proceeding amino acid residues forming the oxy-anion hole and named lid L11 is shown for the latter enzyme to be flexible and change position depending on the presence or absence of glutamine in the glutamine binding site. ...

Cytidine 5¢-triphosphate (CTP) synthase catalyses the ATP-dependent formation of CTP from uridine 5¢-triphosphate using either NH3orL-glutamine as the nitrogen source. The hydrolysis of glutamine is catalysed in the C-terminal glu-tamine amide transfer domain and the nascent NH3that is generated is transferred via an NH3 tunnel [Endrizzi, J.A., Kim, H., Anderson, P.M. & Baldwin, E.P. (2004) Biochemistry43, 6447–6463] to the active site of the N-ter-minal synthasedomainwhere theaminationreactionoccurs....

ThepyrGgene fromLactococcus lactisencodes CTP syn-thase (EC 6.4.3.2), an enzyme converting UTP to CTP. A series of strains were constructed with different levels of pyrGexpression by insertion of synthetic constitutive pro-moters with different strengths in front ofpyrG.These strains expressed pyrGlevels in a range from 3 to 665% relative to thewild-type expression level.Decreasing the level of CTP synthase to 43% had no effect on the growth rate, showing that the capacity of CTP synthase in the cell is in excess in a wild-type strain. ...