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Adaptations of the kinetic properties of mitochondria in striated muscle lacking cytosolic (M) and⁄or mitochondrial (Mi) creatine kinase (CK) iso-forms in comparison to wild-type (WT) were investigated in vitro. Intact mitochondria were isolated from heart and gastrocnemius muscle of WT and single- and double CK-knock-out mice strains (cytosolic (M-CK –⁄ – ), mitochondrial (Mi-CK –⁄ – ) and double knock-out (MiM-CK –⁄ – ), respect-ively).

TheycaC-related gene, ycaCR, is uncharacterized, and has no assigned function to date. Here we clearly showed that the ycaC-related gene from the amphioxus Branchiostoma belcheri, BbycaCR, coded for a novel mem-ber of the isochorismatase superfamily, which is mainly localized in the mitochondrial fraction.

In vertebrates, phosphocreatine and ATP are continuously interconverted by the reversible reaction of creatine kinase in accordance with cellular energy needs. Sarcoma tissue and its normal counterpart, creatine-rich skel-etal muscle, are good source materials to study the status of creatine and creatine kinase with the progression of malignancy.

Three separate creatine kinase (CK) isoform families exist in animals. Two of these (cytoplasmic and mitochondrial) are obligate oligomers. A third, flagellar, is monomeric but contains the residues for three complete CK domains.