The primary purpose of the present study was to investi-gate whether DNA replication at meiotic prophase also requires replication factors, especially proliferating cell nuclear antigen (PCNA). We cloned PCNA cDNAs (CoPCNA) from a cDNA library made from basidia of the basidiomycete,Coprinus cinereus. Interestingly, although CoPCNAis a single-copy gene in the genome, two di€er-entPCNAcDNA species were isolated using degenerate primers and a meiotic cDNA library, and were designated asCoPCNA-aandCoPCNA-b. ...

Previously, the activity of DNA polymeraseawas found in the meiotic prophase I including non-S phase stages, in the basidiomycetes,Coprinus cinereus. To study DNA poly-merasea during meiosis, we cloned cDNAs for the C. cinereusDNApolymeraseacatalytic subunit (p140) and C. cinereusprimase small subunit (p48). Northern analysis indicated that both p140 and p48 are expressed not only at S phase but also during the leptotene/zygotene stages of meiotic prophase I.

Sumoylation is a post-translational modification system that covalently attaches the small ubiquitin-related modifier (SUMO) to target proteins. Ubc9 is required as the E2-type enzyme for SUMO-1 conjugation to tar-gets. Here, we show that Ubc9 interacts with the meiosis-specific RecA homolog, Lim15⁄Dmc1 in the basidiomyceteCoprinus cinereus(CcLim15), and mediates sumoylation of CcLim15 during meiosis.

In this review, we describe the role of a small ubiquitin-like protein modi-fier (SUMO)-conjugating protein, Ubc9, in synaptonemal complex forma-tion during meiosis in a basidiomycete, Coprinus cinereus. Because its meiotic cell cycle is long and naturally synchronous, it is suitable for molecular biological, biochemical and genetic studies of meiotic prophase events.

In eukaryotes, meiosis leads to genetically variable gametes through recom-bination between homologous chromosomes of maternal and paternal ori-gin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombi-nation is unknown.

Meiotic recombination is initiated by DNA double-stranded breaks intro-duced by the SPO11 protein. Despite a decade of research, the biochemical functions of SPO11 remain largely unknown, perhaps because of difficulties in studying the functionally active SPO11.

Recombination of homologous chromosomes is essential for correct reduc-tional segregation of homologous chromosomes, which characterizes meio-sis. To accomplish homologous recombination, chromosomes must find their homologous partners and pair with them within the spatial con-straints of the nucleus.

The second minireview describes the importance of meiotic recombination in meiosis, and the current understanding of this process and its control mecha-nisms. Imperfections in meiotic recombination have been linked withde novo germline mutations, abnor-mal gametes, and infertility.

In meiosis, the accurate segregation of maternal and paternal chromosomes is accomplished by homologous recombination. A central player in meiotic recombination is the Dmc1 recombinase, a member of the RecA⁄Rad51 recombinase superfamily, which is widely conserved from viruses to humans.

One of the most obscure phenomena in modern biology is the near genome-wide displacement of histones that occurs during the postmeiotic phases of spermatogenesis in many species. Here we review the literature to show that, during spermatogenic differentiation, three major molecular mechanisms come together to ‘prepare’ the nucleosomes for facilitated disassembly and histone removal.

Sexual reproduction depends on the success of faithful chromosome trans-mission during meiosis to yield viable gametes. Central to meiosis is the process of recombination between paternal and maternal chromosomes, which boosts the genetic diversity of progeny and ensures normal homo-logous chromosome segregation.

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Meiosis-specific gene discovery in plants: RNA-Seq applied to isolated Arabidopsis male meiocytes

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Apospory appears to accelerate onset of meiosis and sexual embryo sac formation in sorghum ovules

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of anther transcriptomes to identify genes contributing to meiosis and male gametophyte development in rice