Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes
Eur. J. Biochem. 269, 5950–5955 (2002) Ó FEBS 2002
doi:10.1046/j.1432-1033.2002.03323.x
The most active factor VIIa (FVIIa) variants identified to
date carry concurrent substitutions at positions 158, 296and
298 with the intention of generating a thrombin-mimicking
motif, optionally combined with additional replacements
within the protease domain [Persson, E., Kjalke, M. &
Olsen, O. H. (2001)Proc. Natl Acad. Sci. USA98, 13583–
13588]. Here we have characterized variants of FVIIa
mutatedat one or twoof these positions toassess the relative
importance of the individual replacements.
Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes
Egon Persson1 and Ole H. Olsen2
1
Haemostasis Biology and 2Medicinal Chemistry Research IV, Novo Nordisk A/S,...
