HPLC for Pharmaceutical Scientists 2007 (Part 20)

The most widely used analytical separation technique for the qualitative and quantitative determination of chemical mixtures in solution in the pharmaceutical industry is high-performance liquid chromatography (HPLC). However, conventional detectors used to monitor the separation, such as UV, refractive index, fluorescence, and radioactive detectors, provide limited information on the molecular structure of the components of the mixture. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) are the primary analytical techniques that provide structural information on the analytes. NMR is widely recognized as one of the most important methods of structural elucidation, but it becomes cumbersome for the analysis of...