Science reports

The broad objective of research reported here was to develop a multi-publishing workflow for student atlases.

162p runthenight05 01-03-2023 6 3   Download

The focus of this thesis was to study the chemotaxonomic relationship of selected southern Australian marine brown algae of the genera Cystophora and Sargassum. Consequently, this resulted in the isolation and structure elucidation of six new terpenoids from two southern Australian marine brown algae Cystophora moniliformis and Sargassum fallax together with 10 previously reported natural products. As a result of the re-isolation of these known secondary metabolites, updated and complete structural characterisation data could be provided for the first time for 7 of these compounds.

132p runthenight07 01-03-2023 9 3   Download

Epigenetic defects (gain or loss of DNA methylation) of the human ICR1 11p15 domain result in two opposite foetal growth disorders (BWS and SRS) depending on which parental allele is affected. A few deletions within ICR1 have been reported in familial BWS cases with ICR1 gain of methylation, however the mechanism(s) of the DNA methylation defects at ICR1 remains largely unknown in most BWS and SRS patients.

83p runthenight07 01-03-2023 9 3   Download

Those living with disadvantage suffer from higher rates of chronic conditions and multimorbidities. Little work has been done to investigate how this cohort experience patient-centred care and what their outcomes might be. This study showed that as a result of a short course of treatment clients experienced better levels of health and wellbeing and decreased levels of pain and disability. Interview data suggest that this may be due to a combination of therapy, setting and relationships formed within that setting.

177p runthenight04 02-02-2023 5 2   Download

This project report is an analysis of the current situation, needs, and potential medium-and long-term strategies to develop agricultural research and extension in Viet Nam. The report provides a long-term road map for agriculture research and extension assistanceand it will be used as a basis for the development of a forthcoming ADB technical assistance, which will in turn be used as a basis for a proposed investment loan in Agricultural Science and Technology.

281p pechi1412 25-11-2015 36 6   Download

Oxford Centre for Molecular Sciences and the Dyson Perrins Laboratory, Oxford, UK; 2Department of Biochemistry, National Defence Medical Centre, Taipei, Taiwan; 3Department of Pharmacy and Pharmacology, The University of Bath, UK Deacetoxycephalosporin C synthase (DAOCS) catalyses the oxidative ring expansion of penicillin N, the committed step in the biosynthesis of cephamycin C by Streptomyces clavuligerus.

5p system191 01-06-2013 26 3   Download

Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Tunisia; 2De´partement d’Inge´nierie et d’Etude des Prote´ines, CEA, Saclay, France; 3UMR 6560, Universite´ de la Me´diterrane´e, CNRS, Inge´nierie des Prote´ines, Laboratoire de Biochimie, IFR Jean-Roche, Faculte´ de Me´decine Nord, Marseille, France; 4Laboratoire de Neurophysiologie UPRES EA 2647, UFR Sciences, Angers, France BotXIV and LqhaIT are two structurally related long chain scorpion a-toxins that inhibit sodium current inactivation in excitable cells.

11p system191 01-06-2013 46 4   Download

Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Soja, Japan; 2Division of Biological Science, Institute of Scientific and Industrial Research, Osaka University, Japan; 3Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Japan The function of the translation products of six different cDNAs for Acetabularia V-ATPase proteolipid subunit (AACEVAPD1 to AACEVAPD6 ) was examined using a Saccharomyces cerevisiae VMA3-deficient strain that lacked its own gene for one of the proteolipid subunits of V-AT...

8p system191 01-06-2013 40 5   Download

Department of Biochemistry, Eotvos Lorand University, and 2Biotechnology Research Group of the Hungarian Academy of Sciences, ´ ´ ´ ´ Pazmany setany 1/C, Budapest, Hungary The molecular mechanism of the autolysis of rat a-chymotrypsin B was investigated. In addition to the two already known autolytic sites, Tyr146 and Asn147, a new site formed by Phe114 was identified. The former two sites and the latter one are located in the autolysis and the interdomain loops, respectivel

9p system191 01-06-2013 39 4   Download

UMR 5539 (CNRS) Laboratoire de Motilite Cellulaire (Ecole Pratique des Hautes Etudes), Universite de Montpellier, France; ´ Centre de Recherches de Biochimie Macromoleculaire, Montpellier, France; 3Genes and Development Group, Department of Biomedical Sciences, University of Edinburgh, Scotland It is generally assumed that of the six domains that comprise gelsolin, domain 2 is primarily responsible for the initial contact with the actin filament that will ultimately result in the filament being severed.

11p system191 01-06-2013 38 5   Download

Cytidine in the anticodon second position (position 35) and G or U in position 36 of tRNAArg are required for aminoacylation by arginyl-tRNA synthetase (ArgRS) from Escherichia coli. Nevertheless, an arginine-accepting amber suppressor tRNA with a CUA anticodon (FTOR1D26) exhibits suppression activity in vivo [McClain, W.H. & Foss, K. (1988) Science, 241, 1804 –1807]. By an in vitro kinetic study with mutagenized tRNAs, we showed that the arginylation of FTOR1D26 involves C34 and U35, and that U35 can be replaced by G without affecting the activity.

7p system191 01-06-2013 23 3   Download

The Department of Biochemistry and Molecular Biology, 2CREST of Japan Science and Technology Corporation; 3The Department of Nephrology and Endocrinology, Faculty of Medicine, The University of Tokyo, Japan We have cloned cDNA for leukotriene B4 12-hydroxydehydrogenase (LTB 4 12-HD)/15-ketoprostaglandin 13-reductase (PGR) from guinea-pig liver. LTB4 12-HD catalyzes the conversion of LTB4 into 12-keto-LTB4 in the presence of NADP1, and plays an important role in inactivating LTB4.

9p system191 01-06-2013 42 4   Download

Tyrosyl-DNA phosphodiesterase (TDP) cleaves the phosphodiester bond linking the active site tyrosine residue of topoisomerase I with the 3¢ terminus of DNA in topoisomerase I–DNA complexes which accumulate during treatment of cancer with camptothecin. In yeast, TDP mutation confers a 1000-fold hypersensitivity to camptothecin in the presence of an additional mutation of RAD9 gene [Pouliot, J.J., Yao, K.C., Robertson, C.A. & Nash, H.A. (1999) Science 286, 552–555].

8p research12 01-06-2013 40 2   Download

Department of Medical Chemistry, Kochi Medical School, Nankoku, Japan; 2Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan; 3Department of Anatomy and Cell Biology, Kochi Medical School, Nankoku, Japan We previously generated mice lacking aromatase activity by targeted disruption of Cyp19 (ArKO mice), and reported phenotypes of the female mice, showing hemorrhage formation and follicular depletion in the ovary, diminution in uterine size, and bone loss...

9p research12 01-06-2013 42 4   Download

Tetraalkylammonium (TAA) salts are well known reversible inhibitors of cholinesterases. However, at concentrations around 10 mM, they have been found to activate the hydrolysis of positively charged substrates, catalyzed by wild-type human butyrylcholinesterase (EC 3.1.1.8) [Erdoes, E.G., Foldes, F.F., Zsigmond, E.K., Baart, N. & Zwartz, J.A. (1958) Science 128, 92].

8p research12 01-06-2013 31 2   Download

A structuring and eventual exclusion of water surrounding backbone hydrogen bonds takes place during protein fold-ing as hydrophobic residues cluster around such bonds. Taken as an average over all hydrogen bonds, the extent of desolvation is nearly a constant of motion, as revealed by re-examination of the longest all-atom trajectory with explicit solvent [Y.Duan & P.A.Kollman (1998)Science 282, 740].Furthermore, this extent of desolvation is pre-served across native soluble proteins, except for cellular prion proteins....

4p research12 23-04-2013 35 2   Download

The study of protein aggregation saw a renaissance in the last decade, when it was discovered that aggregation is the cause of several human dis-eases, making this field of research one of the most exciting frontiers in science today. Building on knowledge about protein folding energy land-scapes, determined using an array of biophysical methods, theory and simulation, new light is now being shed on some of the key questions in protein-misfolding diseases.

9p dell39 27-03-2013 33 3   Download

Molecular identification of adrenal inner zone antigen as a heme-binding protein Li Min1*, Natallia V. Strushkevich1,2*, Ivan N. Harnastai2, Hiroko Iwamoto3, Andrei A. Gilep1,2, Hiroshi Takemori1, Sergey A. Usanov2, Yasuki Nonaka3, Hiroshi Hori4, Gavin P.

12p dell39 27-03-2013 54 6   Download

Recent research has established NMR as a key method for high-through-put comparative analysis of plant extracts. We discuss recent examples of the use of NMR to provide metabolomic data for various applications in plant science and look forward to the key role that NMR will play in data provision for plant systems biology.

6p galaxyss3 21-03-2013 47 2   Download

The 2006 Global BioMillennium Conference took place in Tbilisi, Georgia, on 13–17 July 2006. The Conference was focused on key aspects of gene expression processes. Characteristic of state-of-the-art research in the life sciences, the invited lectures spanned approaches in cell biology, gene expression, and protein function.

4p galaxyss3 21-03-2013 51 4   Download