Cây Gắm (Gnetum montanum Markgr.) họ Gnetaceae, đã thu hút được sự chú ý nhờ các đặc tính chữa bệnh tiềm năng trong y học cổ truyền. Nghiên cứu này nhằm mục đích đánh giá hoạt tính ức chế enzyme xanthine oxidase (XO) và nitric oxide (NO) của 03 stilbene (isorhapotigenin, gnetifolin E và (+)-ampelopsin F) phân lập từ vỏ và lá của loài G. montanum, cây dược liệu dân gian dùng trị liệu bệnh gút. Cấu trúc hóa học của các hợp chất được xác định dựa trên phổ NMR và ESI-MS.

The present study aimed to determine the chemical compositions and anti-acetylcholinesterase, nitric oxide suppressing activities of Piper longum fruits oil collected from Binh Dinh, Vietnam.

Bài viết trình bày nhận xét kết quả nồng độ Nitric oxide trong khí thở ra ở người bệnh có chồng lấp hen và bệnh phổi tắc nghẽn mạn tính giai đoạn ổn định điều trị ngoại trú tại phòng Quản lý bệnh phổi mạn tính - Bệnh viện Bạch Mai.

"Bài giảng Giá trị của Nitric oxide đường thở và bộ câu hỏi CARATkids trong kiểm soát hen phế quản có viêm mũi dị ứng ở trẻ em" đánh giá tình trạng kiểm soát hen ở trẻ em hen phế quản có viêm mũi dị ứng dựa vào nồng độ nitric oxide đường thở và bộ câu hỏi CARATkids.

Mục tiêu của luận án là xác định mối liên quan giữa FeNO và độ nặng của hen theo cách xếp loại của GINA 2017. Xác định mối liên quan giữa FeNO với các mức KS hen theo GINA 2017 và theo ACT. Xác định điểm cắt của FeNO để phân biệt hen KS tốt và hen không KS theo GINA 2017;...

Mục tiêu của luận án là mô tả đặc điểm FeNO của dân số nghiên cứu và xác định mối liên quan giữa FeNO với các đặc điểm của bệnh nhân bao gồm điểm số ACT và hô hấp ký. Xác định mối liên quan giữa FeNO và độ nặng của hen theo cách xếp loại của GINA 2017.

The effect of a-tocopheryl hemisuccinate (TS) on lipopolysaccharide (LPS)/interferon-c (IFN)-induced nitric oxide production in rat vascular smooth muscle cells (VSMC) was examined. The LPS/IFN-induced NO production was enhanced by TS but not by the other a-tocopherol (a-T) derivatives a-tocopheryl acetate (TA) and a-tocopheryl nicotinate (TN), or a-T itself. a-T, TA and TN inhibited the enhancement by TS of LPS/IFNinduced NO production. The enhancing effect of TS was observed in the presence of LPS, but not IFN, suggesting that TS participates in the LPS-stimulated signal pathway leading to NO production....

Two neuropeptides have been isolated and identi®ed from the secretions of the skin glands of the Stony Creek Frog Litoria lesueuri. The ®rst of these, the known neuropeptide caerulein 1.1, is a common constituent of anuran skin secretions, and has the sequence pEQY(SO3 )TGWMDF-NH2 . This neuropeptide is smooth muscle active, an anal-gaesic more potent thanmorphine and is also thought to be a hormone.

Production of nitric oxide (NO) in response to bacterial lipopolysaccharide (LPS) was investigated using cultures of mouse peritoneal exudate cells (PEC) and the macrophage cell line RAW264.7. In the presence of anti-(interferon-c) (IFN-c), NO production was markedly suppressed in the PEC culture but not in the RAW264.7 culture. In the PEC culture, LPS induced both IFN-cproduction and activation of IFNresponse factor-1, which leads to the gene expression of inducible NO synthase, but neither was induced in the culture of RAW264.7 cells....

The results of the present study show that human platelets take up L-arginine by two transport systems which are compatible with the systems y + and y + L. These Na + -independent transporters have been distinguished by treat-ing platelets with N-ethylmaleimide that blocks selectively systemy + .Systemy + , that accounts for 30–40%of the total transport, is characterized by low affinity forL-arginine, is unaffected byL-leucine, is sensitive to changes of membrane potential and totrans-stimulation. ...

Nitric oxide (NO • ) strongly inhibits the proliferation of human A431 tumour cells. It also inhibits tyrosine phos-phorylation of a 170-kDa band corresponding to the epidermal growth factor receptor (EGFR) and induces the phosphorylation at tyrosine residue(s) of a 58-kDa protein which we have denoted NOIPP-58 (nitric oxide-induced 58-kDa phosphoprotein). The NO • -induced phosphoryla-tion of NOIPP-58 is strictly dependent on the presence of EGF. Phosphorylation of NOIPP-58 and inhibition of the phosphorylation of the band corresponding to EGFR are both cGMP-independent processes. ...

The binding of several alkyl- and aryl-guanidines andN-hydroxyguanidines to the oxygenase domain of inducible NO-synthase (iNOSoxy) was studied by UV⁄Vis difference spectroscopy. In a very general manner, monosubsti-tuted guanidines exhibited affinities for iNOSoxy that were very close to those of the corresponding N-hydroxyguanidines. The highest affinities were observed for the natural substrates,l-arginine and N x -hydroxy-l-arginine (Kd at thelmlevel).

Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate frac-tions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associ-ated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. ...

Metallothioneins (MTs) release boundmetalswhen exposed to nitric oxide. At inflammatory sites, both metallothionein and inducible nitric oxide synthase (iNOS) are induced by the same factors and the zinc released from metallothionein by NO suppresses both the induction and activity of iNOS. In a search for a possible modulatory mechanism of this coexpression of counteracting proteins, we investigated the role of the glutathione redox statein vitro because the oxi-dation state of thiols is involved in themetal binding inCd-S or Zn-Sclusters found in metallothioneins, and NO also binds to reduced glutathione via S-nitrosation....

In rat neuronal nitric oxide synthase, Phe1395 is positioned over the FAD isoalloxazine ring. This is replaced by Trp676 in human cytochrome P450 reductase, a tryptophan in related diflavin reductases (e.g. methionine synthase reduc-tase andnovel reductase 1), and tyrosine inplant ferredoxin-NADP + reductase. Trp676 in human cytochrome P450 reductase is conformationallymobile, andplays akey role in enzyme reduction. Mutagenesis of Trp676 to alanine results in a functional NADH-dependent reductase. ...

Single turnover reactions of the inducible nitric oxide synthase oxygenase domain (iNOSoxy) in the presence of several nona-amino acid N-hydroxy-guanidines and guanidines were studied by stopped-flow visible spectro-scopy, and compared with reactions using the native substrates l-arginine (l-arg) or N x -hydroxy-l-arginine (NOHA).

Nitric oxide (NO • ) has been proposed to be a physiological modulator of cell proliferation, able to promote in most cases cell cycle arrest. In this review I explore the molecular basis of this mechanism of action. The mod-ulatory action of NO • on the intracellular concentration of cGMP and the machinery directly involved in the control of cell cycle progression, inclu-ding the expression and activity of diverse cyclins and cyclin-dependent kinases,

Calmodulin (CaM) is a cytosolic Ca 2+ signal-transducing protein that binds and activates many different cellular enzymes with physiological rele-vance, including the nitric oxide synthase (NOS) isozymes. CaM consists of two globular domains joined by a central linker; each domain contains an EF hand pair.

Cupiennin 1a (GFGALFKFLAKKVAKTVAKQAAKQGAKYVVNKQ-ME-NH2) is a potent venom component of the spider Cupiennius salei. Cupiennin 1a shows multifaceted activity. In addition to known antimicro-bial and cytolytic properties, cupiennin 1a inhibits the formation of nitric oxide by neuronal nitric oxide synthase at an IC50 concentration of 1.3 ± 0.3lm.

Escherichia coliflavorubredoxin (FlRd) belongs to the family of flavodiiron proteins (FDPs), microbial enzymes that are expressed to scavenge nitric oxide (NO) under anaerobic conditions. To degrade NO, FlRd has to be reduced by NADH via the FAD-binding protein flavorubredoxin reduc-tase, thus the kinetics of electron transfer along this pathway was investi-gated by stopped-flow absorption spectroscopy.