Giới thiệu tài liệu
Diabetic Retinopathy (DR) poses a significant global health challenge, being a leading cause of irreversible blindness. While conventional treatments exist, they often carry risks of complications and adverse effects, underscoring the urgent need for safer and more effective adjunctive therapeutic strategies. Traditional Chinese Medicine, such as QiJu-DiHuang Wan (QJDHW), has been clinically applied for DR, yet its underlying molecular mechanisms remain largely unelucidated. This study aims to bridge this knowledge gap by employing advanced computational approaches like network pharmacology and molecular docking to systematically investigate the complex pharmacological actions of QJDHW in the context of DR. By dissecting its multi-component and multi-target interactions, we seek to provide a robust scientific foundation for its therapeutic efficacy.
Đối tượng sử dụng
Researchers in pharmacology, traditional medicine, drug discovery, ophthalmology, and bioinformatics. Clinicians interested in novel therapeutic approaches for diabetic retinopathy.
Nội dung tóm tắt
This study systematically investigated the molecular mechanisms of QiJu-DiHuang Wan (QJDHW) in the adjunctive treatment of Diabetic Retinopathy (DR) using an integrated network pharmacology and molecular docking approach. The methodology involved comprehensively analyzing compounds present in QJDHW and DR-related target proteins sourced from various open-access databases. Key steps included constructing protein-protein interaction networks and compound-target networks using STRING and Cytoscape, followed by identifying relevant signaling pathways through GO and KEGG enrichment analysis utilizing DAVID. The final stage involved evaluating molecular interactions and binding affinities via Autodock Vina and BIOVIA Discovery Studio 2021. The results revealed 10 bioactive compounds within QJDHW that interact with 10 crucial target proteins, all implicated in key signaling pathways relevant to DR pathophysiology. The subsequent molecular docking simulations demonstrated strong binding affinities between these identified compounds and their respective targets, suggesting a robust molecular basis for their therapeutic actions. This research provides a valuable theoretical foundation for understanding QJDHW's efficacy in DR, identifies potential novel therapeutic targets, and significantly contributes to advancing the scientific development of traditional medicine formulations for DR treatment.
