Bài giảng Bộ xương tế bào, được biên soạn với mục tiêu nhằm giúp các bạn sinh viên có thể kể tên và trình bày chức năng của ba loại protein bộ xương tế bào; Mô tả được đặc điểm cấu trúc của siêu sợi actin và các protein kết hợp với siêu sợi actin; Trình bày được cơ chế hoạt động của siêu sợi actin và các protein kết hợp với siêu sợi actin. Mời các bạn cùng tham khảo!

Bài viết trình bày mô tả đặc điểm hình dạng sóng hai pha (BWP) và khả năng dự báo tình trạng DIC ở bệnh nhân suy gan cấp (SGC). Đối tượng và phương pháp nghiên cứu: Nghiên cứu mô tả trên 40 bệnh nhân SGC điều trị tại Trung tâm Hồi sức tích cực bệnh viện Bạch Mai từ tháng 07/2023 đến tháng 07/2024. Thu thập mẫu nghiên cứu ngay tại thời điểm vào trung tâm và chưa sử dụng chất chống đông máu, mẫu nghiên cứu được phân tích trên máy CS 5100, thuốc thử Dade Actin FSL.

Bài viết mô tả mối liên quan giữa biến đổi chỉ số phân tích dạng sóng cục máu đông (CWA) với tình trạng rối loạn đông máu ở bệnh nhân suy gan cấp (SGC). Đối tượng và phương pháp nghiên cứu: Nghiên cứu mô tả trên 40 bệnh nhân SGC điều trị tại Trung tâm Hồi sức tích cực bệnh viện Bạch Mai từ tháng 07/2023 đến tháng 07/2024.

Đề tài ngiên cứu nhằm mô tả các hình thái lâm sàng và giải phẫu bệnh của ung thư ống tiêu hóa không thuộc biểu mô; mô tả các phương pháp phẫu thuật và đánh giá kết quả điều trị phẫu thuật của ung thư ống tiêu hóa không thuộc biểu mô. Mời các bạn cùng tham khảo nội dung chi tiết.

Mục tiêu của đề tài là mô tả các hình thái lâm sàng và giải phẫu bệnh của ung thư ống tiêu hóa không thuộc biểu mô; mô tả các phương pháp phẫu thuật và đánh giá kết quả điều trị phẫu thuật của ung thư ống tiêu hóa không thuộc biểu mô. Mời các bạn cùng tham khảo nội dung chi tiết.

UMR 5539 (CNRS) Laboratoire de Motilite Cellulaire (Ecole Pratique des Hautes Etudes), Universite de Montpellier, France; ´ Centre de Recherches de Biochimie Macromoleculaire, Montpellier, France; 3Genes and Development Group, Department of Biomedical Sciences, University of Edinburgh, Scotland It is generally assumed that of the six domains that comprise gelsolin, domain 2 is primarily responsible for the initial contact with the actin filament that will ultimately result in the filament being severed. Other actin-binding regions within domains 1 and 4 are involved in gelsolin’s severing and subsequent capping activity....

The ¯uorescence resonance energy transfer parameter, f, is de®ned as the eciency of the energy transfer normalized by the quantum yield of the donor in the presence of acceptor. It is possible to characterize the ¯exibility of the protein matrix between the appropriate ¯uorescent probes by monitoring the temperature dependence of f. The intermonomer ¯exibility of the Ca-actin and Mg-actin ®laments was characterized by using this method at pH values of 6.5 and 7.4.

Ikkai&Ooi [Ikkai,T.&Ooi,T. (1966)Biochemistry5, 1551± 1560] made a thorough study of the e€ect of pressure on G- and F-actins. However, all of the measurements in their study were made after the release of pressure. In the present experiment in situ observations were attempted by using eATP to obtain further detailed kinetic and thermodynamic information about the behaviour of actin under pressure.

A marked pH-dependent interaction with F-actin is an important property of typical members of the actin depolymerizing factor (ADF)/cofilin family of abundant actin-binding proteins. ADF/cofilins tend to bind to F-actin with a ratio of 1 : 1 at pH values around 6.5, and to G-actin at pH 8.0. We have investigated the mechan-ism for the pH-sensitivity.

Peptides corresponding to the N-terminus of skeletal myosin light chain 1 (rsMLC1 1–37) and the short loop of human cardiacb-myosin (hcM398–414) have been shown to interact with skeletal F-actin by NMR and fluorescence measurements. Skeletal tropomyosin strengthens the binding of the myosin peptides to actin but does not interact with the peptides. The binding of peptides cor-responding to the inhibitory region of cardiac troponin I (e.g. hcTnI128–153) to F-actin to form a 1 : 1 molar complex is also strengthened in the presence of tropomyo-sin. ...

The interaction of heat shock protein with molecular mass 25 kDa (HSP25) and its point mutants S77D + S81D (2D mutant) and S15D + S77D + S81D (3D mutant) with intact and thermally denatured actinwas analyzedbymeans of fluorescence spectroscopy and ultracentrifugation. Wild typeHSP25 did not affect the polymerization of intact actin. The HSP25 3D mutant decreased the initial rate without affecting the maximal extent of intact actin polymerization. G-actin heated at 40–45
C was partially denatured, but retained its ability to polymerize....

BAP31 is a 28-kDa integral membrane protein of the endoplasmic reticulumwhose cytosolicdomaincontains two caspase recognition sites that are preferentially cleaved by initiator caspases,such as caspase-8. Recently,we reported that the caspase-resistant BAP31 inhibited Fas-mediated apoptotic membrane fragmentation and the release of cytochromec from mitochondria in KB epithelial cells (Nguyen M.,Breckenridge G.,Ducret A &Shore G. (2000) Mol. Cell.Biol.20,6731–6740).

Hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) are the most common hereditary cardiac conditions. Both are frequent causes of sudden death and are often associated with an adverse disease course.Alpha-cardiac actinis one of the disease genes where different mis-sense mutations have been found to cause either HCM or DCM. We have tested the hypothesis that the protein-folding pathway plays a role in dis-ease development for two actin variants associated with DCM and six asso-ciated with HCM....

Titin is known to interact with actin thin filaments within the I-band region of striated muscle sarcomeres. In this study, we have used a titin fragment of 800 kDa (T800) purified from striated skeletal muscle to measure the effect of this interaction on the functional properties of the actin– myosin complex. MALDI-TOF MS revealed that T800 contains the entire titin PEVK (Pro, Glu, Val, Lys-rich) 1 domain. In the presence of tropomyosin–troponin, T800 increased the sliding velocity (both average and maximum values) of actin filaments on heavy-meromyosin (HMM)-coated surfaces and dramatically decreased the number of stationary filaments. ...

Connective tissue growth factor (CTGF/CCN2) is an immediate early gene-encoded polypeptide modulating cell growth and collagen synthesis. The importance of CTGF/ CCN2 function is highlighted by its disregulation in fibrotic disorders. In this study, we investigated the regulation and signaling pathways that are required for various stimuli of intracellular signaling events to induce the expression of the endogenous CTGF/CCN2gene in smooth muscle cells.

The phosphorylation-dephosphorylation of serine and threonine residues of calponin is known tomodulatein vitro its interactionwithF-actin and is thought to regulate several biological processes in cells, involving either of the calponin isoforms. Here, we identify, for the first time, tyrosine-phosphorylated calponin h3 within COS 7 cells, before and after their transfection with the pSV vector containing cDNA encoding the cytoplasmic, Src-related, tyrosine kin-ase, Fyn.

Plectin, a large and widely expressed cytolinker protein, is composed of several subdomains that harbor binding sites for a variety of different interaction partners. A canonical actin-binding domain (ABD) comprising two calponin homology domains (CH1 and CH2) is located in proximity to its amino terminus. However, the ABD of plectin is unique among actin-binding proteins as it is expressed in the form of distinct, plectin isoform-specific versions. We have determined the three-dimensional structure of two distinct crystalline forms of one of its ABD versions (pleABD/2a) from mouse, to a resolution of 1.95 and 2.0 A ˚....

Inorganic phosphate (Pi) and cofilin⁄actin depolymerizing factor proteins have opposite effects on actin filament structure and dynamics. Pi stabilizes the subdomain 2 in F-actin and decreases the critical concentration for actin polymerization. Conversely, cofilin enhances disorder in subdomain 2, increases the critical concentration, and accelerates actin treadmilling.

Angiomotin, an 80 kDa protein expressed in endothelial cells, promotes cell migration and invasion, and stabilizes tube formationin vitro. Angiomotin belongs to a new protein family with two additional members, Amotl-1 and Amotl-2, which are characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs.

Previous cross-linking studies [Kim E, Bobkova E, Hegyi G, Muhlrad A & Reisler E (2002)Biochemistry41, 86–93] have shown that site-specific cross-linking among F-actin monomers inhibits the motion and force generation of actomyosin. However, it does not change the steady-state ATPase parameters of actomyosin.