ĐNH L NG Ị ƯỢ E.COLI GI ĐNHẢ Ị
B NG PH NG PHÁP MPNẰ ƯƠ
Phạm Thị Vinh Hoa
Phòng Xét nghiệm trung tâm
- Indicators of microbial water quality:
•General (process) microbial indicators
•Faecal indicators (such as E. coli)
•Index organisms and model organisms.
US Environmental Protection Agency
The EPA has determined that E. coli are one of the best indicators for the presence of
potentially pathogenic bacteria. Because E. coli monitoring does not measure the actual
pathogens, the assessment is not foolproof, however, it is a good approach for assessing the
likelihood of risks to human health. Monitoring for these indicator organisms is an easy and
economical method for citizens or professionals to assess health risks due to bacterial
contamination of surface waters.
http://www.usawaterquality.org/volunteer/ecoli/june2008manual/chpt2_ecoli.pdf
Escherichia coli (E. coli): Thermophilic coliforms that produce indole from tryptophan, but also
defined now as coliforms able to produce β-glucuronidase (although taxonomically up to
10% of environmental E. coli may not). Most appropriate group of coliforms to indicate faecal
pollution from warm-blooded animals.
http://www.who.int/water_sanitation_health/dwq/iwachap13.pdf
T I SAO C N KI M TRA Ạ Ầ Ể ESCHERICHIA COLI?
ESCHERICHIA COLI
http://en.wikipedia.org/wiki/Escherichia_coli
-Hình que, Gram-âm,
không sinh bào t ử
-Lên men đng lactose ườ
sinh h i và acid ơ 44ởoC
-S có m t c a ự ặ ủ E.coli
kh ng đnh s nhi m ẳ ị ự ễ
khu n t phânẩ ừ
TÀI LI U THAM KH OỆ Ả
TCVN 5999:1995 (ISO 5667/10:1992):
Ch t l ng n c. L y m u. H ng d n l y m u n c th iấ ượ ướ ấ ẫ ướ ẫ ấ ẫ ướ ả
TCVN 8880:2011 (ISO 19458:2006):
Ch t l ng n c. L y m u đ phân tích vi sinh v tấ ượ ướ ấ ẫ ể ậ
TCVN 6187-2:1996 (ISO 9308/2:1990):
Ch t l ng n c. Phát hi n và đm vi khu n coliform, vi khu n ấ ượ ướ ệ ế ẩ ẩ
coliform ch u nhi t và escherichia coli gi đnh. Ph n 2: Ph ng ị ệ ả ị ầ ươ
pháp nhi u ng (s có xác su t cao nh t)ề ố ố ấ ấ