Xây dựng và thẩm định phương pháp định lượng aflatoxin B1 trong dược liệu, ngũ cốc bằng sắc ký lỏng hiệu năng cao

Aflatoxin B1, a highly toxic mycotoxin produced by Aspergillus species, poses significant health risks due to its prevalence in food and herbal drugs, especially in humid tropical climates like Vietnam. Chronic exposure to Aflatoxin B1, even at low doses, is associated with severe health issues, including liver cancer. Accurate and sensitive quantification methods are crucial for ensuring the safety of herbal drugs and cereals. Given the low concentrations of Aflatoxin B1 typically found, advanced analytical techniques such as high-performance liquid chromatography (HPLC) are indispensable. This study addresses a critical gap by developing and validating a robust HPLC method for quantifying Aflatoxin B1 in herbal drugs and cereals.

Researchers and professionals in analytical chemistry, food and drug safety, toxicology, and quality control laboratories.

This study successfully developed and validated a high-performance liquid chromatography (HPLC) method for the accurate quantification of Aflatoxin B1 in various herbal drugs and cereals. The methodology adhered strictly to ICH guidelines, ensuring robust validation across critical parameters including specificity, repeatability, linearity, accuracy, and precision. Key chromatographic conditions involved a Waters C18 column (50 mm × 2.1 mm, 1.7 µm), an isocratic mobile phase composed of aqueous 0.1% phosphoric acid and acetonitrile (0.2% (33:67)), a flow rate of 0.2 ml/min, and detection via PDA-UV at 354 nm. Sample preparation utilized ultrasonic extraction with 30% ethanol, followed by solid-phase extraction to concentrate the analyte, enabling detection at low concentrations. The developed method demonstrated excellent performance characteristics, with clear separation of Aflatoxin B1 from other components. When applied to 100 samples of herbal drugs and cereals collected from Hanoi and Ha Giang, detectable levels of Aflatoxin B1 were found in two samples, representing approximately 2% of the analyzed batch, with concentrations ranging from 5.08 to 10.4 µg/kg. These findings align with previous reports on mycotoxin prevalence. This validated high-performance liquid chromatography method provides a precise and reliable tool for routine monitoring of Aflatoxin B1 contamination, safeguarding public health and food safety. It also establishes a solid foundation for future research into mycotoxin presence and mitigation strategies in traditionally used herbal products and staple food sources.