Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1
An extracellular lipase, LipA, extracted fromAcinetobacter
sp. RAG-1 grown on hexadecane was purified and proper-ties of the enzyme investigated. The enzyme is released into
the growth medium during the transition to stationary
phase. The lipase was harvested from cells grown to sta-tionary phase, and purified with 22% yield and 10-fold
purification. The protein demonstrates little affinity for
anionexchange resins,withcontaminatingproteins removed
by passing crude supernatants over a Mono Q column....