J. Sci. Dev. 2011, 9 (Eng.Iss. 1): 108 - 111 HANOI UNIVERSITY OF AGRICULTURE
Short communication
Development of TGMS line 135S
Dòng mẹ TGMS 135s
Vu Hong Quang1, Nguyen Van Hoan2, Pham Van Cuong2, Mai Van Tan1
1Rice Research Institute – Hanoi University of Agriculture, Vietnam
2Faculty of Agronomy, Hanoi University of Agriculture, Vietnam
Corresponding author email: quangvh@vietlai.com.vn
Received date: 03.03.2011 Accepted date: 20.04.2011
TÓM TẮT
Dòng mẹ 135s được tạo ra từ tổ hợp lai giữa hai dòng mẹ 103s/Pei Ải 64s và được lai trở lại một
lần từ năm 2003. Đến năm 2006 đã chọn được dòng thuần được đặt tên là dòng 135s. Dòng mẹ 135s
có kiểu hình thuộc kiểu hình kiểu cây mới, ở giai đoạn mẫn cảm từ bước 4 - 6 của phân hóa đòng,
dòng chuyển hóa bất dục hoàn toàn khi nhiệt độ trung bình của môi trường ≥ 240C và hữu dục cao khi
nhiệt độ trung bình của môi trường < 230C. Dòng mẹ 135s có khả năng kết hợp chung cao với các
dòng bố và con lai F1 cho ưu thế lai cao hơn so với đối chứng F1 là giống lúa lai hai dòng nhập nội
Bồi tạp sơn thanh (BTST) từ Trung Quốc.
Từ khóa: Chọn tạo giống, dòng mẹ TGMS, lúa lai hai dòng, ưu thế lai.
SUMMARY
A new thermo- sensitive genic male sterile (TGMS) line, 135S was selected from a cross between
TGMS 103S line and TGMS line, Peiai64S, using pedigree selection method from 2003 to 2006.
The135S line is of a new plant type with short growth duration (78-80) and has complete sterility when
exposed to extreme temperatures, good capacity for obtaining outside pollens This TGMS line also
showed high grain yield (4.86 ton ha-1) in self multiplication at fertility stages. The GA3 amount
required for helping this line obtain outside pollens in F1 seed production was low (140 g per ha).
Furthermore, 135S exhibits good combining ability with male parents, i.e. the F1 hybrid combinations
between the 135S line and 6 restorer lines (R45, R50, R63, 9311, No 44, No 154) showed high heterosis
for grain yield (2.0 – 4.0 ton per ha). Based on the high grain yield of maintenance of TGMS seed, high
yield of F1 seed production and F1 hybrid performance, TGMS 135S line could be recommended for
use in two-line hybrid system across the country.
Key words: Heterosis, hybrid rice, TGMS line.
A new thermo- sensitive genic male sterile (TGMS) line 135S was developed by the Rice Research
Institute (RRI), Hanoi University of Agriculture from 2003 to 2006. The cross between TGMS lines 103S
and Peiai64S was made; the F1 was then backcrossed to 103S for one generation. The pedigree method of
selection was applied in the backcross progeny for early maturity, sterility, stability and suitability for
developing two-line hybrids (Fig.1).
TGMS line 135S has short growth duration, new plant type, high yield of seed production, and high
outcrossing rate. The main characteristics of the TGMS line 135S are presented in Table 1 and Table 2.
Under field experiment conditions at RRI, the growth duration of 135S from sowing to flowering varies
from 78 to 80 days and depends on sowing date. Seed multiplication at Bacha District – Lao Cai province
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showed that 135S had growth duration of 120 days (about 80 days from sowing to 20% flowering).
Average seed yield was about 4.86 tons ha-1.
103S x Peiai 64S
103S x F1
BC1F1
Pedigree selection
……….
135S
Pure lines and testcross
Pure lines and seed production
Year 2003 (Spring and Summer)
Year 2004 (Spring and Summer)
Spring 2005 and Summer 2005
Year 2006
Figure 1. Development of TGMS line 135S
Table 1. Morpho-agromonomic characters of 135S line under field condition in summer season
No. Characters 135S line 103S line (Check)
1 Time from sowing to flowering (days) 78 – 80 81-83
2 Plant height (cm) 86.6 89.7
3 Effective tillers hill -1 4.3 4.6
4 Number of leaves 14.3 15.0
5 Time of flowering 9h30 9h30
6 Outcrossing rate (%) 74.3 81.3
7 Grains panicle-1 239.5 212.5
8 Plant type New plant type Normal plant type
9 Fertility rate Complete fertility Complete fertility
10 Ability of receiving outside pollens (from restorers) Good Good
11 1000 grain weight (g) 22.0 23.0
12 Stigma exertion rate (one sides) (%) 37.2 38.6
13 Stigma exertion rate (both sides) (%) 41.8 45.4
14 Grain filling rate to the effect of GA3 (%) 78.9 81.9
Table 2. Yield and yield components of 135S line in summer in 2006
at Bac Ha district – Laocai province
TGMS
Lines
Active
tillers
hill-1
Number
of
spikelets/
panicle-1
Number
of filled
grains/
panicle-1
Fertile
rate (%) at
17.00C ≤ t0 ≤
22.50C
Individual
yield
(g/ plant-1)
1000-
grains weight
(g)
Yield
(tons/ ha-1)
135s8.0±2 240±4 140±3 85.0 23.0±2 22.0 4.86±2
103s
(Check) 7.2±3 215±4 125±3 81.0 21.0±2 23.0 3.86±2
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Vu Hong Quang, Nguyen Van Hoan, Pham Van Cuong, Mai Van Tan
The TGMS line 135S was also evaluated for its combining ability with available restorer lines.
Combining ability test showed that the F1 hybrids between 135S and restorer lines have normal fertility
rate (Table 3). Further extensive cross tests showed that hybrids of 135S with the pollen lines have normal
fertility and high individual grain yield. The results suggest that 135S is a potentially promising TGMS
parent for the two – line system of hybrid rice breeding.
6 F1 hybrids (between 135s and 6 restorer lines) showed high heterosis (Table 4). Compared with the check
variety, the characters of seed set per panicle, 1000-grain weight and individual yield had a high standard
heterosis.
Without applying GA3, the outcrossing rate (cross fertilization) of 103S is higher than 135s line. In
contrast, with GA3 treatment, the outcrossing rate of 135S line was higher than 103s (Table 5). The greatest
effect of GA3 on the outcrossing habit of 135S line was three days after flowering.
Table 3. Yield and yield components of 6 F1 hybrid combinations
Characters
F1 hybrid
Combinations
# effective tillers
Number of spikelets
per panicle
Number filled
grainsper panicle
Percent of filled grains
(%)
1000 – grain
weight
Individual yield
(g plant-1)
Harvest index
Grain yield
(1000 kg ha-1)
135S/R50 4.7 293.1 259.0 88.4 25.5 21.4 0.52 94.4
135S/R45 3.9 307.4 265.1 86.2 23.5 18.5 0.53 78.8
135S/9311 4.0 204.5 173.8 87.3 28.1 18.0 0.47 64.5
135S/R63 4.2 233.6 204.0 84.6 28.5 19.4 0.39 80.6
135S/44 3.8 227.6 193.1 84.8 26.6 21.2 0.52 71.7
135S/154 4.4 199.5 187.2 93.8 27.1 18.1 0.49 78.0
BTST(Check) 4.0 185.4 173.6 93.6 21.5 14.9 0.41 49.3
Table 4. Heterosis for yield components of 6 F1 hybrid combinations involving TGMS line 135S
Number of tillers Number of filled grains
per panicle11000-grain weight Individual yield
F1 hybrid
Combinations HB % HS % HB % HS % HB % HS % HB % HS %
135S/R50 46.9 17.5 37.1 49.2 4.0 9.3 68.5 36.6
135S/R45 17.2 -15.0 57.4 52.7 11.8 23.3 57.3 24.2
135S/9311 48.1 0.0 16.7 0.1 -3.1 30.7 78.2 20.8
135S/R63 -6.7 5.0 29.4 17.5 0.0 32.6 -26.2 3.2
135S/44 -7.3 -5.0 -23.9 11.2 4.6 37.7 1.0 42.3
135S/154 -18.5 10.0 39.7 7.8 -1.0 33.5 28.6 21.5
HS: Heterosis Standard
HB: Heterosis Beltiosis
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Development of TGMS Line 135S
Table 5. Effect of GA3 on the ability of receiving outside pollen of 103S and 135S lines
Outcross rate – with GA3
(%)
Outcrossing rate – non GA3
(%)
Day after flowering
103S135S103S135S
1 56.90 50.33 81.96 78.93
2 44.50 39.07 75.15 67.99
3 35.50 32.03 63.19 59.63
4 26.60 23.07 51.13 36.40
5 17.30 14.20 35.03 15.73
6 11.40 9.13 19.50 20.06
7 4.90 7.17 7.90 15.70
LSD 0.05% 4.80 2.61 4.20 3.98
CV% 9.60 5.30 5.80 5.40
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