Ectoine-mediated protection of enzyme from the effect of pH and temperature stress: a study using Bacillus halodurans xylanase as a model
In this work, the protective effect of ectoines against pH stress and high temperature has been investigated using an alkaline active xylanase from Bacillus halodurans as a model. The disaccharide, trehalose—a well-known stabiliser for biomolecules (Kaushik and Bhat 2003), has also been included in the study. The enzyme was selected as a model because it is efficiently active at high pH and is highly stable in the pH range of 5.5–10.5 at 50 °C.
