Khảo sát độc tính của khối Bacillus spp. trên động vật thực nghiệm

Probiotics have gained significant attention for their health-promoting properties, particularly Bacillus spp., which are widely utilized in various health supplements. However, the safety profile of novel probiotic strains, especially those intended for human consumption, is a critical prerequisite for their successful application. While many commercial probiotic products employ foreign-sourced strains, there is a growing need for locally isolated strains that are effective and safe. This study addresses this gap by investigating the acute and subchronic toxicity of a novel Bacillus spp. mixture isolated from Vietnamese individuals, providing essential safety data for its potential therapeutic development.

Scientists and researchers in microbiology, toxicology, and pharmaceutical development; professionals in the probiotic and functional food industries; healthcare providers interested in evidence-based probiotic therapies.

This study aimed to comprehensively evaluate the acute and subchronic toxicity of a proprietary Bacillus spp. mixture (comprising B. subtilis, B. coagulans, and B. clausii) isolated from the gastrointestinal tract of healthy Vietnamese individuals. The research employed động vật thực nghiệm models to ascertain the safety of this novel probiotics blend. For acute toxicity assessment, Swiss albino mice were orally administered the Bacillus spp. mixture at doses up to 5000 mg/kg (2x10^10 CFU/kg), revealing no signs of toxicity or mortality, thus precluding the determination of an LD50. The subchronic toxicity study involved Albino rabbits, which received daily oral doses of 1 g/kg/day and 5 g/kg/day for 28 days. Throughout this period, no significant changes were observed in the animals' body weight, general health, or hematological parameters compared to the control group. Although mild hepatic degeneration and minor inflammatory cell infiltration were noted in some liver tissues of the treated rabbits during histopathological examination, biochemical markers for liver and kidney function (AST, ALT, GGT, Creatinine, Ure) remained within normal physiological ranges and showed no statistically significant differences from the control group. These findings strongly suggest that the Bacillus spp. VN mixture is safe for consumption at the tested doses. This research provides crucial evidence supporting the safety of this locally sourced Bacillus spp. for use in health products, potentially offering an affordable and effective alternative for addressing health issues, including vaginal infections, and contributing to the development of indigenous probiotics strains.