Phân lập và tuyển chọn các chủng vi khuẩn Acetobacter xylinum có khả năng tạo màng cellulose từ nước ép áo hạt quả vải

Cellulose is a ubiquitous natural polysaccharide with diverse applications across various fields due to its robust properties and versatile utility. Among its forms, bacterial cellulose (BC) has garnered significant attention for its exceptional purity, strong nanofibrillar structure, high water retention capacity, and biocompatibility, making it ideal for medical, food, cosmetic, and environmental applications. Acetobacter xylinum is a prominent BC-producing bacterium, frequently found in natural fruit ferments. In Vietnam, litchi (Litchi chinensis) is an abundant fruit, and its seed coat juice represents a promising, underutilized agricultural by-product. Leveraging this resource for BC production offers a sustainable pathway to create high-value products. This research therefore focuses on isolating and identifying Acetobacter xylinum strains from litchi seed coat juice and investigating key factors influencing bacterial cellulose membrane production.

Researchers and professionals in microbiology, biotechnology, food science, pharmaceuticals, and biomaterials interested in sustainable biopolymer production and waste valorization.

This study aimed to isolate and identify Acetobacter xylinum strains capable of producing robust bacterial cellulose membranes from litchi extract, specifically focusing on litchi seed coat juice, and subsequently optimize the production conditions. The methodology involved isolating bacteria from the biofilm formed during natural fermentation of litchi juice using the Winogradsky-Beijerinck method. Microbial identification was rigorously conducted through 16S rRNA gene sequencing and BLAST analysis, which revealed that the isolated strain exhibited 100% sequence similarity to Gluconacetobacter entanii strain HWW100 and Acetobacter sp. PA2.2. Optimization experiments explored the effects of litchi juice concentration (10-100%), medium pH (4.7, 5.2, 5.7), and incubation time (1-11 days) on membrane production. The results indicate that optimal membrane production was achieved in a medium containing 60% litchi juice, at a pH of 5.2, with an incubation period of 7 days. This research demonstrates the high capacity of the isolated bacterial strain to produce bacterial cellulose under these specific optimal conditions, underscoring the significant potential of utilizing litchi-derived substrates. These findings pave the way for sustainable and cost-effective production of bacterial cellulose, with broad application value in the food industry, pharmaceuticals, and biomaterials. Future work should concentrate on upscaling the fermentation process and thoroughly evaluating the mechanical, physical, and chemical properties of the produced membranes.