Bài giảng Sinh học phân tử: Chương 2 Sự sao chép DNA, cung cấp cho người học những kiến thức như: Khái niệm; Sự sao chép của DNA; Sửa sai trong sao chép và khi không sao chép. Mời các bạn cùng tham khảo!

Mục đích của nghiên cứu nhằm góp phần đánh giá chính xác hiệu quả sử dụng enzyme Pwo-pol ở quy mô phòng thí nghiệm đồng thời định hướng cải tiến hiệu quả enzyme trong ứng dụng ở Việt Nam. Mời các bạn cùng tham khảo nội dung chi tiết.

Đề tài “Nghiên cứu sản xuất, tinh sạch Pfu DNA polymerase tái tổ hợp từ Escherichia coli" với mục tiêu sản xuất Pfu DNA polymerase chất lượng cao góp phần tích cực trong việc phục vụ các nghiên cứu về sinh học phân tử trong nước.

Đề tài "Nhân dòng, biểu hiện, tinh sạch và xác định hoạt tính của protein KOD DNA polymerase tái tổ hợp ở E. coli" với mục tiêu tạo ra KOD DNA polymerase tái tổ hợp bằng phương pháp đơn giản, từ đó có thể sản xuất lượng lớn enzyme này, làm chủ về mặt công nghệ ở Việt Nam.

Bài giảng Công nghệ DNA tái tổ hợp giới thiệu tới các bạn một số nội dung chính sau: Các enzyme dùng trong tạo dòng phân tử, điện di gel, khuếch đại in vitro DNA bằng phản ứng chuỗi polymerase (PCR),... Mời các bạn cùng tham khảo để nắm bắt nội dung chi tiết cảu bài giảng.

The naturally synchronous plasmodia of myxomycetes synthesize poly(b-L-malic acid), which carries out cell-specific functions. In Physarum polycephalum, poly(b-L-malate) [the salt form of poly(b-L-malic acid)] is highly concentrated in the nuclei, repressing DNA synthetic activity of DNA polymerases by the formation of reversible complexes. To test whether this inhibitory activity is cell-cycle-dependent, purified DNA polymerase a of P. polycephalum was added to the nuclear extract and the activity was measured by the incorporation of [3H]thymidine 5¢-monophosphate into acid precipitable nick-activated salmon testis DNA. Maximum DNA synthesis by the reporter was measured in S-phase, equivalent to a minimum of inhibitory activity....

Previously, the activity of DNA polymeraseawas found in the meiotic prophase I including non-S phase stages, in the basidiomycetes,Coprinus cinereus. To study DNA poly-merasea during meiosis, we cloned cDNAs for the C. cinereusDNApolymeraseacatalytic subunit (p140) and C. cinereusprimase small subunit (p48). Northern analysis indicated that both p140 and p48 are expressed not only at S phase but also during the leptotene/zygotene stages of meiotic prophase I.

Host species specificity of the polyomaviruses simian virus 40 (SV40) and mouse polyomavirus (PyV) has been shown to be determined by the host DNA polymerase a-primase complex involved in the initiation of both viral and host DNA replication. Here we demonstrate that DNA repli-cation of the related human pathogenic polyomavirus JC virus (JCV) can be supportedin vitro by DNA polymerase a-primase of either human or murine origin indicating that the mechanism of its strict species specificity differs from that of SV40 and PyV. Our results indicate that this may be due to differences in the interaction of JCV and SV40 large T antigens with the DNA replication initiation complex....

We have previously reported that sulfoquinovosylmonoacylglycerol (SQMG) is a potent inhibitor of mammalian DNA polymerases. DNA polymeraseb(pol b) is one of the most important enzymes protecting the cell against DNA damage by base excision repair. In this study, we charac-terized the inhibitory action of SQMG against rat pol b. SQMG competed with both the substrate and the template-primer for binding to polb.

The chi (v)and psi(w) subunits of Escherichia coliDNA polymerase III formaheterodimer that is associatedwith the ATP-dependent clamp-loadermachinery. InE. coli,thev:w heterodimer serves as a bridge between the clamp-loader complex and the single-strandedDNA-binding protein. We determined the crystal structure of thev:wheterodimer at 2.1 A ˚ resolution. Although neitherv(147 residues) nor w (137 residues) bind to nucleotides, the fold of each protein is similar to the folds of mononucleotide-(v) or dinucleotide-(w) binding proteins, without marked similarity to the structures of the clamp-loader subunits....

Little is known about the functions of DNA polymerasek (Pol k) recently identified in mammals. From the genomic sequence information of rice andArabidopsis, we found that Polkmay be the only member of the X-family in higher plants. We have succeeded in isolating the cDNA and recombinant protein of Polkin a higher plant, rice (Oryza sativaL. cv. Nipponbare) (OsPolk). OsPolkhad activities of DNA polymerase, terminal deoxyribonucleotidyl trans-ferase and deoxyribose phosphate lyase, a marker enzyme for base excision repair. ...

DNA polymeraseeco-operates with polymerasesaanddin the replicative DNA synthesis of eukaryotic cells. We describe here a specific physical interaction between DNA polymerase eand RNA polymerase II, evidenced by reciprocal immunoprecipitation experiments. The interacting RNA polymerase II was the hyperphosphorylated IIO form implicated in tran-

Enhancer of rudimentary (ER) is a small protein that has a unique amino acid sequence and structure. Its highly conserved gene has been found in all eukaryotic kingdoms with the exception of fungi. ER was proposed to be involved in the metabolism of pyrimidines and was reported to act as a transcriptional repressor in a cell type-specific manner.

Cell survival depends not only on the ability to repair damaged DNA but also on the capability to perform DNA replication on unrepaired or imperfect templates. Crucial to this process are specialized DNA polym-erases belonging to the Y family. These enzymes share a similar catalytic fold in their N-terminal region, and most of them have a less-well-con-served C-terminus which is not required for catalytic activity.

Within this work we describe the purification and biochemical characteriza-tion of a ddNTP-sensitive DNA polymerase purified from mungbean (Vigna radiatacv B1, L.) seeds at 18 days after fertilization, when 70% of the nuclei are reported to be in the endoreduplicated state.

HIV-1 reverse transcriptase (RT) has two associated activities, DNA poly-merase and RNase H, both essential for viral replication and validated drug targets. Although all RT inhibitors approved for therapy target DNA poly-merase activity, the search for new RT inhibitors that target the RNase H function and are possibly active on RTs resistant to the known non-nucleoside inhibitors (NNRTI) is a viable approach for anti-HIV drug development.

PolC is one of two essential replicative DNA polymerases inBacillus subtil-is and other Gram-positive bacteria. The 3D structure of PolC has recently been solved, yet it lacks the N-terminal region. For this PolC region of 230 residues, both the structure and function are unknown.

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques

Phương pháp Dideoxy hay còn gọi là phương pháp gián đoạn chuỗi (chain-determination method) là một phương pháp xác định trình tự DNA được Frederick Sanger phát triển vào năm 1975. Nguyên tắc cơ bản của phương pháp Dideoxy dựa vào hoạt động của enzyme DNA polymerase trong quá trình tổng hợp DNA. Enzyme DNA polymerase xúc tác gắn các nucleotide vào mạch đơn DNA đang tổng hợp ở vị trí 3'có chứa nhóm -OH tự do, khi gặp nucleotide không có nhóm 3'-OH thì phản ứng tổng hợp bị dừng lại. ...