Báo cáo khoa học: Structural determinants of protein stabilization by solutes The importance of the hairpin loop in rubredoxins
Despite their high sequence homology, rubredoxins fromDesulfovibrio
gigasandD. desulfuricansare stabilized to very different extents by com-patible solutes such as diglycerol phosphate, the major osmolyte in the
hyperthermophilic archaeonArchaeoglobus fulgidus[Lamosa P, Burke A,
Peist R, Huber R, Liu M Y, Silva G, Rodrigues-Pousada C, LeGall J,
Maycock C and Santos H (2000)Appl Environ Microbiol66, 1974–1979].
The principal structural difference between these two proteins is the
absence of the hairpin loop in the rubredoxin fromD. desulfuricans....