Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability
A large number of proteins, found experimentally to have
different optimum pH of maximal stability, were studied to
reveal the basic principles of their preferenence for a par-ticular pH. The pH-dependent free energy of folding was
modeled numerically as a function of pH as well as the net
charge of the protein. The optimum pH was determined in
the numerical calculations as the pH of the minimum free
energy of folding. The experimental data for the pH of
maximal stability (experimental optimum pH) was repro-ducible (rmsd¼0.73)....
