MINISTRY OF EDUCATION
AND TRAINING
VIETNAM ACADEMY OF
SCIENCE AND TECHNOLOGY
GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY
-----------------------------
Ninh Thi Ngoc
STUDY ON CYTOTOXIC ACTIVITY OF SOME CANCER
CELL LINES OF ISOLATED COMPOUNDS FROM THREE
SOFT CORALS SINULARIA NANOLOBATA, SINULARIA
LEPTOCLADOS, SINULARIA CONFERTA COLLECTED
FROM THE SEA AREA OF CENTRAL VIETNAM
Major: Biochemistry
Code: 9.42.01.16
SUMMARY OF BIOLOGY DOTORAL THESIS
Ha Noi - 2021
This thesis was completed at: Graduate university of Science and
Technology - Vietnam Academy of Science and Technology
- Institute of Biotechnology
- Institute of Marine Biochemistry
Advisor 1: PhD. Nguyen Hoai Nam
Advisor 2: PhD. Tran My Linh
Reviewer 1: Assoc. Prof. PhD. Nguyen Dinh Thang
Reviewer 2: Assoc. Prof. PhD. Tran Thu Huong
Reviewer 3: PhD. Bui Thi Thuy Luyen
This thesis will be defended at Graduate University of Science and
Technology - Vietnam Academy of Science and Technology at hour
date month 2021.
The thesis can be found in:
- The Library of Graduate University of Science and Technology, Vietnam
Academy of Science and Technology.
- National Library of Vietnam.
- Institute of Biotechnology
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INTRODUCTION
1. The urgency of the thesis
In recent years, many countries have exploited bioactive substances
from marine organisms to serve researches to find drugs to treat dangerous
diseases such as cancer, hepatitis, and diseases infections and viruses. Up to
this point, a number of pharmaceuticals derived from marine organisms
have reached the users' hands, such as Cytarabine, Vidarabine, Eribulin,
Trabectedin ... To achieve this achievement, research institutes around the
world has screened the biological activity of millions of compounds from
marine species, and invested financial resources and time in pre-clinical and
clinical research phases for potential compounds.
With the advantage of owning a long coastline of over 3.260 km along
with many islands and bays, Vietnam has great potential for exploiting a
diverse marine resources, rich in both species composition and storage
amount. However, up to now, there are not many studies searching for
valuable active ingredients from Vietnamese marine organisms and limited
in the in vivo activity test step and research on drug-cell interaction
mechanisms cancer. The studies are currently in the early stages compared
with other countries in the region and far behind the advanced countries.
The reason is that there are still some difficulties such as: surveying and
collecting samples of marine organisms requires modern equipment,
compounds isolated from marine organisms often have very small
concentrations, complex structures impurities, some compounds that are
easy to decompose right in the analysis process. Therefore, an urgent
requirement for our country is to develop research to step by step systemize
the chemical composition and biological activity of marine species.
Genus Sinularia is one of the genus of soft corals that is interested in
research by many scientists around the world. Up to now, there have been
many studies on the chemical composition and biological activities of many
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compounds isolated from soft corals belonging to this genus. However,
studies on many soft corals of the genus Sinularia such as S. nanolobata, S.
leptoclados, S. conferta in Vietnam are few and almost no systematic and
methodical studies on these species mentioned above. Stemming from the
above fact, I chose the thesis topic "Study on cytotoxic activity of some
cancer cell lines of isolated compounds from three soft corals Sinularia
nanolobata, Sinularia leptoclados, Sinularia conferta collected from the
sea area of Central Vietnam" was chosen.
2. The objectives of the thesis
- Determine the chemical composition of three soft coral species S.
nanolobata, S. conferta, S. leptoclados collected in the central sea of
Vietnam.
- Detecting active substances with cytotoxic activity in soft corals
studied, orienting their application for biomedical and pharmacological
studies.
3. The main contents of the thesis
- Determine the scientific names of three soft corals collected from the
sea area of Central Vietnam by molecular markers.
- Isolation and Determination of chemical structures of isolated
compounds from three soft corals S. nanolobata, S. conferta, S. leptoclados.
- Evaluation of cytotoxic activity in vitro of isolated compounds.
- Evaluation of cytotoxicity mechanism of some typical compounds.
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CHAPTER 1: OVERVIEW
This chapter provides an overview domestic and international studies on
the extraction of natural compounds from marine organisms in cancer
treatment, research on cytotoxic activity, general characteristics of the soft
corals and genus Sinularia, about the chemical composition and biological
activity of soft corals genus Sinularia
1.1. Test to evaluate cytotoxic activity
1.2. Natural compounds from marine organisms in cancer treatment
1.3. Introduction to soft corals
1.3.1. Characteristics of soft corals
1.3.2. Overview of soft corals of the genus Sinularia
1.3.3. Application of molecular indicators in the classification of soft
corals
1.3.4. Study on the biological activity of isolated compounds from soft
corals of the genus Sinularia
Statistics of published studies show that compounds isolated from soft
corals genus Sinularia mainly include sesquiterpen, diterpen and steroid
compounds. Many of these compounds exhibit interesting biological
activity such as cytotoxic, anti-inflammatory, antibacterial, antiviral,
neuroprotective and antioxidant activities.
CHAPTER 2: SUBJECTS AND METHODS
2.1. Subjects
Sample of S. nanolobata Verseveldt, 1977 was collected at Lang Co,
Hue, Vietnam (4/2015).
Sample of S. leptoclados Ehrenberg, 1834 was collected at Con Co,
Quang Tri, Vietnam (5/2016).
Sample of S. conferta Dana, 1846 was collected at Con Co, Quang Tri,
Vietnam (5/2015).