MINISTRY OF EDUCATION AND TRAINING MINISTRY OF DEFENSE
VIETNAM MILITARY MEDICAL UNIVERSITY
NGÔ THU H NGẰ
ANTICANCER EFFECTS OF VACCINE STRAIN
MEASLES VIRUS IN COMBINATION WITH
NIMOTUZUMAB IN TREATMENT OF LARYNGEAL
CANCER IN VITRO AND IN VIVO
Major: Biomedical Science
Code: 9720101
SUMMARY OF DOCTORAL THESIS IN MEDICINE
HA NOI - 2020
THE THESIS IS COMPLETED AT THE
VIETNAM MILITARY MEDICAL UNIVESITY
Supervisors:
1. Prof. Dr. NGUY N LĨNH TOÀNỄ
2. Assoc. Prof. Dr. H ANH S NỒ Ơ
Reviewer 1: Prof. Dr. VĂN ĐÌNH HOA
Reviewer 2: Assoc. Prof. Dr. TR NH TU N DŨNGỊ Ấ
Reviewer 3: Assoc. Prof. Dr. PH M TU N C NHẠ Ấ Ả
The thesis is defended in front of the scientific committee at the
Vietnam Military Medical University at on 2020.
The thesis can be found at:
- National Library
- Library of the Vietnam Military Medical University
1
INTRODUCTION
Cancer is a major health problem and is increasingly concerned in
all countries of the world. Head and neck cancer (HNS) is a group of
malignant tumors that develop in this part of the body, and 90% of
HNS has squamous cell carcinoma (HNSCC). Globally, HNS ranks
seventh with 4.8% of all newly diagnosed cancers. Oncolytic virus
(OLV) therapy is based on the main mechanism that OLVs have the
ability to specifically enter and replicate in cancer cells of the tumor
and cause cell lysis, promote cell apoptosis and stimulate immune
response against cancers.
Nimotuzumab is a monoclonal antibody targeting the epidermal
growth receptor (EGFR), which is effective against angiogenesis,
inhibits cell proliferation, induces apoptosis, and promotes cell
sensitivity to radiation and chemotherapy.
We conducted the project “Anticancer effects of vaccine strain
measles virus in combination with nimotuzumab in the treatment of
laryngeal cancer in vitro and in vivo” with two objectives.
1. To evaluate the anticancer effect of measles vaccine virus in
combination with Nimotuzumab in vitro.
2. To evaluate the anticancer effect of measles vaccine virus in
combination with Nimotuzumab on nude mouse model with
head and neck cancer (in vivo).
Necessity of the project:
Investigation of the anticancer effect of measles vaccine virus
(MeV) in combination with Nimotuzumab both in vitro and on a
nude mouse model will serve as a basis for further studies on the
mechanism of combined antitumor effect of MeV and Nimotuzumab,
2
as well as for clinical trials using a MeV and Nimotuzumab
combination to treat cancer patients in general and HNS in particular.
New contribution of the thesis:
This thesis is the first study to evaluate the anticancer effect of the
combination of MeV and Nimotuzumab against HNS on Hep2 cells
as well as on a nude mouse model with head and neck cancer. This is
the basis for further experimental studies and clinical trials for cancer
treatment.
Layout of the thesis:
The thesis has 150 pages, including: Introduction (2 pages),
Chapter 1: Literature review (36 pages), Chapter 2: Materials and
Methods (27 pages), Chapter 3: Results (47 pages), Chapter 4:
Discussion (35 pages), Conclusion (2 pages), Recommendations (1
page). The thesis has 175 references (171 references in English).
CHAPTER 1: LITERATURE REVIEW
1.1. Head and neck cancer
Head and neck cancer (HNS) is a group of malignant tumors that
develop in this part of the body including mouth, nose,
throat, larynx, sinuses, or salivary glands, cancer of the oral cavity,
sinuses next to nose and tongue. The incidence of HNS is increasing
both in Vietnam and in some parts of the world. HNS has a bad
prognosis, is dangerous and has many major complications.
The main risk factors for HNS include tobacco, alcohol
consumption, HPV infection (for oral cancer), EBV infection (for
throat cancer). The oncogenes in HNSCC are associated with four
main functional pathways: cell proliferation, squamous
epithelialization, cell survival and invasion/metastasis.