Báo cáo khóa học: Conformational changes of b-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles A fluorescence and CD study
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The effect ofb-lactoglobulin encapsulation in sodium bis (2-ethylhexyl) sulfosuccinate reverse micelles on the envi-ronment of protein and on Trp was analysed at different water contents (x0).CDdataunderlined thedistortionof the b-sheet and a less constrained tertiary structure as the x0 increased, in agreement with a concomitant red shift and a decrease in the signal intensity obtained in steady-state fluorescence measurements.
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