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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:11

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Trehalose synthase (TreS) catalyzes the reversible inter-conversion of trehalose (glucosyl-a,a-1,1-glucose) and maltose (glucosyl-a1-4-glucose). TreS was purified from the cytosol ofMycobacterium smegmatisto give a single protein band on SDS gels with a molecular mass of68 kDa. However, active enzyme exhibited a molecular mass of 390 kDa by gel filtration suggesting that TreS is a hexa-mer of six identical subunits.

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Nội dung Text: Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme

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