Xây dựng phương pháp bào chế cao khô định chuẩn quercetin từ dược liệu trai hoa trần

Medicinal extracts are crucial in traditional medicine, yet quality and standardization, particularly for dry extracts, present ongoing challenges. Murdannia nudiflora (L.) Brenan, a widely utilized herb in traditional Asian medicine, is rich in the valuable flavonoid quercetin, recognized for its diverse therapeutic properties including cardiovascular protection, anti-inflammatory, and anti-ulcer effects. Traditional preparation methods often result in inconsistent efficacy. To maximize the therapeutic potential and advance pharmaceutical development, establishing a reliable process for a standardized dry extract of this herb is imperative. This research aims to develop and optimize a method for preparing such an extract from Murdannia nudiflora, ensuring consistent quality and a defined quercetin content, thereby providing a foundation for high-quality pharmaceutical applications.

Researchers in pharmaceutical science, pharmacognosy, and traditional medicine; pharmaceutical manufacturers; and regulatory bodies.

The study focused on establishing a systematic and reproducible method for producing a high-quality dry extract from Murdannia nudiflora (L.) Brenan, specifically targeting the standardization of its active compound, quercetin. This involved optimizing each stage of the preparation process, from initial extraction to final drying and quality control. The dried extract was obtained from a liquid crude extract, which was first concentrated to a 3:1 ratio and purified. Subsequent optimization involved investigating various excipients, drying times, and temperatures to achieve optimal physical and chemical characteristics. Cassava starch at 25% was identified as the most effective excipient. Static drying at 90°C for 8 hours yielded the best results. Quality parameters were rigorously evaluated according to the Vietnamese Pharmacopoeia V, including sensory attributes, loss on drying, fineness, Thin-Layer Chromatography (TLC), and High-Performance Liquid Chromatography (HPLC) for both qualitative and quantitative assessment of quercetin. The developed method successfully produced a standardized dry extract characterized by its dry, loose, homogeneous texture, dark brown color, characteristic herbal odor, and sweet taste, meeting all specified quality control criteria (loss on drying ≤ 5%, fineness ≥ 90%). Most significantly, HPLC analysis confirmed a clear quercetin peak with 99.19% purity and a consistent quercetin content of at least 80 mg/g (dry weight basis). These findings provide a crucial scientific foundation for the further research and development of Murdannia nudiflora into pharmaceutical products, enhancing its therapeutic efficacy and promoting its consistent use in medicine.