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Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum

Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum

The interactions between photosystem I and five charge mutants of plastocyanin from the cyanobacteriumPhormi-dium laminosumwere investigatedin vitro. The dependence of the overall rate constant of reaction,k2, on ionic strength was investigated using laser flash photolysis. The rate con-stant of the wild-type reaction increased with ionic strength, indicating repulsion between the reaction partners. Remov-inganegativechargeonplastocyanin(D44A) acceleratedthe reactionandmade it independentof ionic strength; removing a positive charge adjacent to D44 (K53A) had little effect....
10 trang
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Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein

Báo cáo khoa học: Analysis of the effect of potato carboxypeptidase inhibitor pro-sequence on the folding of the mature protein

Protein folding can be modulatedin vivo by many factors. While chaperones act as folding catalysts and show broad substrate specificity, some pro-peptides specifically facilitate the folding of the mature protein to which they are bound. Potato carboxypeptidase inhibitor (PCI), a 39-residue pro-tein carboxypeptidase inhibitor, is synthesized in vivo as a precursor protein that includes a 27-residue N-terminal and a seven-residue C-terminal pro-regions.
10 trang
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Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing

Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing

Formamidopyrimidine-DNA glycosylase (Fpg protein) of Escherichia coliis a DNA repair enzyme that excises oxi-dized purine bases, most notably the mutagenic 7-hydro-8-oxoguanine, from damaged DNA. In order to identify specific contacts between nucleobases of DNA and amino acids from theE. coliFpg protein, photochemical cross-linking was employed using new reactive DNA duplexes containing 5-[4-[3-(trifluoromethyl)-3H-diazirin-3-yl]phe-nyl]-2¢-deoxyuridine dU* residues near the 7-hydro-8-oxoguanosine (oxoG) lesion. ...
5 trang
54 lượt xem
3 lượt tải
Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II

Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II

We have studied the reaction native«denatured for the 33-kDa protein isolated from photosystem II. Sucrose and glycerol have profound effects on pressure-induced unfolding. The additives shift the equilibrium to the left; they also cause a significant decrease in the standard volume change (DV). The change in DVwas related to the sucrose and glycerol concentrations.
8 trang
95 lượt xem
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Báo cáo khoa học: Emergence of a subfamily of xylanase inhibitors within glycoside hydrolase family 18

Báo cáo khoa học: Emergence of a subfamily of xylanase inhibitors within glycoside hydrolase family 18

The xylanase inhibitor protein I (XIP-I), recently identified in wheat, inhib-its xylanases belonging to glycoside hydrolase families 10 (GH10) and 11 (GH11). Sequence and structural similarities indicate that XIP-I is related to chitinases of family GH18, despite its lack of enzymatic activity. Here we report the identification and biochemical characterization of a XIP-type inhibitor from rice.
11 trang
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Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II

Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II

To elucidate the domains on the extrinsic 23 kDa protein involved inelectrostatic interactionwiththeextrinsic33 kDa protein in spinach photosystem II, we modified amino or carboxyl groups ofthe 23 kDa protein to unchargedmethyl ester groups with N-succinimidyl propionate or glycine methyl ester in the presence ofa water-soluble carbodi-imide, respectively.TheN-succinimidyl propionate-modified 23 kDa protein did not bind to the 33 kDa protein associ-atedwithPSIImembranes, whereas the glycinemethyl ester-modified 23 kDa protein completely bound. ...
10 trang
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Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins

Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins

The protein disulfide isomerase is known to play important roles in the folding of nascent polypeptides and in the formation of disulfide bonds in the endoplasmic reticulum (ER). In this study, we cloned a gene of a novel protein disulfide isomerase family from soybean leaf (Glycine maxL. Mer-rill. cv Jack) mRNA.
12 trang
63 lượt xem
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Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin

Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin

Spinophilin, a neuronal scaffolding protein, is essential for synaptic trans-mission, and functions to target protein phosphatase-1 to distinct subcellu-lar locations in dendritic spines. It is vital for the regulation of dendritic spine formation and motility, and functions by regulating glutamatergic receptors and binding to filamentous actin.
10 trang
80 lượt xem
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Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase

Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase

In vitrorefolding of maltodextrin glucosidase, a 69 kDa monomericEscher-ichia coli protein, was studied in the presence of glycerol, dimethylsulfox-ide, trimethylamine-N-oxide, ethylene glycol, trehalose, proline and chaperonins GroEL and GroES.
11 trang
54 lượt xem
4 lượt tải
Báo cáo khoa học: X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis

Báo cáo khoa học: X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis

The 1,3(4)-b-d-glucanases of glycoside hydrolase family 16 provide useful examples of versatile yet specific protein–carbohydrate interactions. In the present study, we report the X-ray structures of the 1,3(4)-b-d-glucanase Phanerochaete chrysosporiumLaminarinase 16A in complex withb-glucan products from laminarin (1.6 A˚ ) and lichenin (1.1 A ˚ ) hydrolysis. The G6G3G3G glucan, in complex with the enzyme, showed ab-1,6 branch in the acceptor site.
12 trang
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báo cáo khoa hoc : PP3 forms stable tetrameric structures through hydrophobic interactions via the C-terminal amphipathic helix and undergoes reversible thermal dissociation and denaturation

báo cáo khoa hoc : PP3 forms stable tetrameric structures through hydrophobic interactions via the C-terminal amphipathic helix and undergoes reversible thermal dissociation and denaturation

The milk protein proteose peptone component 3 (PP3), also called lacto-phorin, is a small phosphoglycoprotein that is expressed exclusively in lactating mammary tissue. The C-terminal part of the protein contains an amphipathic helix, which, upon proteolytic liberation, shows antibacterial activity. Previous studies indicate that PP3 forms multimeric structures and inhibits lipolysis in milk.
12 trang
57 lượt xem
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báo cáo khoa học: " ZINC-INDUCED FACILITATOR-LIKE family in plants: lineage-specific expansion in monocotyledons and conserved genomic and expression features among rice (Oryza sativa) paralogs"

báo cáo khoa học: " ZINC-INDUCED FACILITATOR-LIKE family in plants: lineage-specific expansion in monocotyledons and conserved genomic and expression features among rice (Oryza sativa) paralogs"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: ZINC-INDUCED FACILITATOR-LIKE family in plants: lineage-specific expansion in monocotyledons and conserved genomic and expression features among rice (Oryza sativa) paralogs
22 trang
61 lượt xem
5 lượt tải
Báo cáo y học: "The role of RNA folding free energy in the evolution of the polymerase genes of the influenza A virus"

Báo cáo y học: "The role of RNA folding free energy in the evolution of the polymerase genes of the influenza A virus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The role of RNA folding free energy in the evolution of the polymerase genes of the influenza A virus...
0 trang
67 lượt xem
4 lượt tải
Báo cáo y học: "Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2dimensional gel electrophoresis"

Báo cáo y học: "Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2dimensional gel electrophoresis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2dimensional gel electrophoresis...
11 trang
69 lượt xem
3 lượt tải

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