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Chương 3. Đng hc phn ng to sinh khi
3.1. Cac khai niê 󰈨m cơ ba󰈖n
3.2. Qua t rinh ta󰈨o sinh kho󰈘 i vi sinh va 󰈨t
3.3. Ky thua 󰈨t nuo i ca󰈘 y thêo mê󰈖
3.4. Ky thu a 󰈨t nuo i ca󰈘 y thêo mê󰈖 co bo󰈜 sungcơ cha󰈘 t
3.5. Phương trinh Monod va Đo 󰈨ng ho󰈨c ta󰈨o sinh kho󰈘 i vi
sinh va 󰈨t
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BIOLOGICAL REACTIONS
•BIOREACTORS
Lab Scale Bioreactor
Industrial Scale Bioreactor
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Fermentation Process
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Major Functions of a Bioreactor
1) Provide operation free from
contamination;
2) Maintain a specific temperature;
3) Provide adequate mixing and aeration;
4) Control the pH of the culture;
5) Allow monitoring and/or control of
dissolved oxygen;
6) Allow feeding of nutrient solutions and
reagents;
7) Provide access points for inoculation
and sampling;
8) Minimize liquid loss from the vessel;
9) Facilitate the growth of a wide range of
organisms.
Ref;(Allman A.R., 1999: Fermentation
Microbiology and Biotechnology)
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Biotechnological Processes Of Growing
Microorganisms In A Bioreactor
1) Batch culture: microorganisms are inoculated into a fixed
volume of medium and as growth takes place nutrients are
consumed and products of growth (biomass, metabolites)
accumulate.
2) Semi-continuous:
fed batch
-gradual addition of concentrated
nutrients so that the culture volume and product amount are
increased (e.g. industrial production of baker’s yeast);
Perfusion
-addition of medium to the culture and withdrawal of an
equal volume of used cell-free medium (e.g. animal cell
cultivations).
3) Continuous: fresh medium is added to the bioreactor at the
exponential phase of growth with a corresponding withdrawal of
medium and cells. Cells will grow at a constant rate under a
constant condition.
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