Báo cáo khoa học: An active triple-catalytic hybrid enzyme engineered by linking cyclo-oxygenase isoform-1 to prostacyclin synthase that can constantly biosynthesize prostacyclin, the vascular protector
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It remains a challenge to achieve the stable and long-term expression (in human cell lines) of a previously engineered hybrid enzyme [triple-catalytic (Trip-cat) enzyme-2; Ruan KH, Deng H & So SP (2006) Biochemistry45, 14003–14011], which links cyclo-oxygenase isoform-2 (COX-2) to prostacy-clin (PGI2) synthase (PGIS) for the direct conversion of arachidonic acid into PGI2through the enzyme’s Trip-cat functions.
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