Identification and characterization of B¢¢-subunits of protein
phosphatase 2 A in
Xenopus laevis
oocytes and adult tissues
Evidence for an independent N-terminal splice variant of PR130
and an extended human PR48 protein
Ilse Stevens
1
, Veerle Janssens
1
, Ellen Martens
1
, Stephen Dilworth
2
, Jozef Goris
1
and Christine Van Hoof
1
1
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Leuven, Belgium;
2
Department of Metabolic
Medicine, Imperial College Faculty of Medicine, Hammersmith Hospital, London, UK
Protein phosphatase 2A is a phosphoserine/threonine
phosphatase implicated in many cellular processes. The core
enzyme comprises a catalytic and a PR65/A-subunit. The
substrate specificity and subcellular localization are deter-
mined by a third regulatory B-subunit (PR55/B, PR61/B¢
and PR72/130/B¢¢). To identify the proteins of the B¢¢ family
in Xenopus laevis oocytes, a prophase Xenopus oocyte cDNA
library was screened using human PR130 cDNA as a probe.
Three different classes of cDNAs were isolated. One class is
very similar to human PR130 and is probably the Xenopus
orthologue of PR130 (XPR130). A second class of clones
(XN73) is identical to the N-terminal part of XPR130 but
ends a few amino acids downstream of the putative splicing
site of PR130. To investigate how this occurs, the genomic
structure of the human PR130 gene was determined. This
novel protein does not act as a PP2A subunit but might
compete with the function of PR130. The third set of clones
(XPR70) is very similar to human PR48 but has an N-ter-
minal extension. Further analysis of the human EST-data-
base and the human PR48 gene structure, revealed that the
human PR48 clone published is incomplete. The Xenopus
orthologue of PR48 encodes a protein of 70 kDa which like
the XPR130, interacts with the A-subunit in GST pull-down
assays. XPR70 is ubiquitously expressed in adult tissues and
oocytes whereas expression of XPR130 is very low in brain
and oocytes. Expression of XN73 mainly parallels XPR130
with the exception of the brain.
Keywords: phosphatase; PP2A; Xenopus laevis; cell cycle.
Reversible protein phosphorylation is a key mechanism that
regulates a number of different cellular events. The phos-
phorylation state of a protein is dependent on the opposing
activities of kinases and phosphatases. Among the phos-
phatases, protein phosphatase 2A is a major serine/threo-
nine protein phosphatase involved in many cellular events,
such as signal transduction, DNA replication, transcription,
translation, apoptosis and the cell cycle [reviewed in 1,2].
The core enzyme of PP2A is a heterodimer consisting of a
catalytic (C) and structural (PR65/A) subunit. Different
mechanisms are known to regulate the phosphatase activity.
The PR65/A subunit serves as a scaffolding protein that
interacts with a third regulatory subunit (B-subunit). There
is evidence that PP2A is an obligate trimer in vivo [3,4],
although other evidence suggests that a substantial propor-
tion of PP2A can also exist in vivo as a dimer [5]. The
association of a B subunit with the AC core dimer can result
in altered substrate specificity, catalytic activity and subcel-
lular localization. To date, three different B-subunit families
have been described in eukaryotes: PR55/B, PR61/B¢and
PR72/130/B¢¢. Each family of B-subunits harbours different
genes, some giving rise to different splice variants. PP2A
activity is also subject to regulation by post-translational
modifications, such as methylation. Methylation occurs on
the carboxyl group of the C-terminal Leu309 by a specific
methyltransferase [6,7] and is reversible by a methylesterase
[8–10]. There is evidence that methylation is required for the
association of certain regulatory B-subunits with the AC
heterodimer [11–13].
PP2A has been shown to be required at various stages of
the eukaryotic cell cycle [reviewed in 1]. Xenopus laevis
oocytes are often used as a model system to study specific
aspects of the cell cycle. Oocytes taken from the ovary of the
frog are arrested in prophase of the first meiotic division.
Addition of progesterone results in meiotic progression and
a second arrest in metaphase of the next meiotic division. At
this stage, oocytes are also called eggs and are ready to be
fertilized. The whole process is called oocyte maturation.
Mitotic [14] and DNA replicating events [15] can be studied
Correspondence to J. Goris, K.U. Leuven, Faculteit Geneeskunde,
Gasthuisberg O/N, Afdeling Biochemie, Herestraat 49,
B-3000 Leuven, Belgium.
Fax: + 32 16 345995, Tel.: + 32 16 345794,
E-mail: jozef.goris@med.kuleuven.ac.be
Abbreviations: PP2A, protein phosphatase type 2 A; OA, okadaic
acid; CDK1, cyclin dependent kinase 1; GVBD, germinal vesicle
breakdown; PP1, protein phosphatase 1; GST, glutathione
S-transferase; IVTT, in vitro transcription-translation.
Note: The reported nucleotide sequence data of the B¢¢ subunits of
PP2A are available in the DDBJ/EMBL/GenBank under the accession
numbers: AY043260 (Xenopus PR130), AY043259 (Xenopus PR70),
AY043258 (Xenopus N73) and BK000521 (Human PR70).
(Received 27 August 2002, revised 14 November 2002,
accepted 25 November 2002)
Eur. J. Biochem. 270, 376–387 (2003) FEBS 2003 doi:10.1046/j.1432-1033.2003.03398.x
easily in specific egg extracts and X. laevis is also a useful
model to study embryogenesis, as all the material required
for early development are accumulated in the oocyte. A role
for PP2A in the cell cycle was initially suggested through
experiments using okadaic acid (OA), an inhibitor of PP1
and PP2A. Induction of meiotic oocyte maturation
occurred after injection of OA into Xenopus [16] or starfish
oocytes [17]. This inhibitory activity of PP2A is shown to be
due to the presence of a trimeric form of PP2A containing
the PR55/B-subunit [18]. Furthermore, immuno-depletion
of PP2A from Xenopus egg extracts resulted in strong
inhibition of chromosomal DNA replication [19].
Currently, we are investigating the role of PP2A during
the cell cycle using X. laevis oocytes as model system. To
achieve this goal, an inventory of the different PP2A
subunits from X. laevis oocytes is required. At the moment,
two PP2A
C
(aand b) [20], one PR65/A [21,22], one PR55/B
[22] and two PR61/B¢subunits [23,24] have been cloned
from X. laevis. It has been shown that the PR65/A subunit
in oocytes is exclusively of the b-type, whereas the PR55/B is
most similar to the human PR55aisoform [22]. However,
nothing is currently known about the X. laevis PR72/130/
B¢¢ family of PP2A subunits. As the B subunits are
important components of the control of PP2A activity, it
is essential that the complement of B subunits expressed in
the oocyte is defined. In mammalia, three different B¢¢
family members have been identified: human PR72/130 [25],
mouse PR59 [26] and human PR48 [27]. PR72 was initially
identified as the third subunit of a novel trimeric form of
PP2A from rabbit skeletal muscle [28]. By cloning the
cDNA from a human heart cDNA library, a second cDNA
was isolated coding for a protein of 130 kDa with the same
deduced C-terminal protein sequence as PR72 but a
different N-terminal region. PR72 is specifically expressed
in skeletal muscle and heart, while PR130 is ubiquitous. The
function of PR72 and PR130 is currently not known. PR59
[26] and PR48 [27] were identified in a yeast two-hybrid
assay as proteins interacting with p107 (a relative of the
retinoblastoma protein) and cdc6, respectively. Both pro-
teins were found to show high sequence identity with the
PR72 protein especially in their central part. They have both
been found to be involved in DNA replication and the G1/S
phase transition of the cell cycle.
It has been suggested that PP2A may be a tumour
suppressor gene, as inactivation of the regulatory PR65/A
subunit by gene mutation [29] or ablation [30,31] occurs
frequently in human tumours. Moreover, the cellular target
for the tumour promoter okadaic acid has been shown to be
PP2A (and its relatives). In spinocerebellar ataxia, a
neurodegenerative disorder, an expanded CAG repeat has
been identified immediately upstream of the PR55bgene
that might affect PR55bexpression and might be implicated
in the aetiology of the disease [32]. To evaluate the role of
the PP2A as a putative tumour suppressor gene it is
important that the genomic structures of the PP2A subunits
are defined.
To identify all of the proteins of the PR72/130/B¢¢ family
in oocytes, a prophase Xenopus oocyte cDNA library was
screened using a full-length human PR130 cDNA as a
probe. Three different classes of cDNAs were isolated and
analysed. The first is the Xenopus orthologue of PR130,
the second proved to be a novel splice variant and the third is
the Xenopus orthologue of PR48. Moreover, analysis of the
structure of the latter made it possible to improve the current
knowledge of the mammalian PR48 cDNA. To extend these
findings, the genomic structures of the human PR130 and
PR48 genes (PPP2R3A and PPP2R3B, according to the
human gene nomenclature) were also analysed. Together
the data illustrate the complexity of PP2A regulation by a
still growing list of B-type regulatory subunits.
Materials and methods
cDNA library screening and sequencing
AXenopus oocyte kgt10 library [33] was screened using full-
length human PR130 cDNA as the probe. This specific
probe was obtained by performing PCR using Pwo
polymerase (Roche), the start primer (5¢-TGGGTCGAC
TATGGCAGCAACTTACAGACTTGTGG-3¢)andthe
stop primer (5¢-TCGGATCCATTCTTCATCCACT
GATTGAAGC-3¢) and human PR130 cDNA (accession
number L12146) as template. The PCR product was
subsequently labelled with a random priming DNA label-
ling kit (Roche). One million plaques were analysed,
positives were isolated and the plaques purified as described
previously [34]. Isolated clones were digested with EcoRI
and cloned into the pBluescript SK
–
vector. Nucleotide
sequences were determined with a Thermo Sequenase
fluorescent labelled primer cycle sequencing kit and the
A.L.F. Express Sequenator (Amersham-Pharmacia Bio-
tech). In addition to the original clones, we used subclones
obtained by restriction enzyme digestion as templates for
DNA sequencing.
5¢-RACE technique
5¢-RACE reactions were performed on about 1.5 lgoftotal
RNA of prophase X. laevis oocytes with 1 U of AMV-
reverse transcriptase (5¢/3¢RACE kit, Roche). For the first
strand cDNA synthesis, to amplify 5¢ends of XN73,
the primer 5¢-GACCACTGCTTTCTCCTCCACC-3¢was
used. A nested primer (5¢-GATTTCCTGTTAAAAGA
GTCAGGACGG-3¢) was used together with the oligo(dT)-
anchor primer for amplification with Pwo polymerase
(Roche). Subsequently, two other consecutive PCR reac-
tions were performed, each time using a nested primer
(5¢-ACACTCCTCACCACCAACTACC-3¢for PCR 2 and
5¢-CTCTCCACCTATTATTTTATGAGTAACATCC-3¢
for PCR 3) and the anchor primer supplied in the 5¢-RACE
kit. For amplification of specific XPR130 5¢-end RACE
fragments, the primer 5¢-TGGACTGACAGTTGGAG
TAAGG-3¢was used for the RT reaction, and the primer
5¢-GTTCCTGAGCAAAGTCTTCAATG-3¢was used
together with the oligo(dT)-anchor primer in the first PCR
reaction and the primers 5¢-TCTCCAAATCCAACAGC
AATTT-3¢and 5¢-GACCACTGCTTTCTCCTCCACC-3¢
were used for the two consecutive PCR reactions together
with the anchor primer. Similar 5¢-RACE reactions were
performed to amplify the 5¢ends of XPR48. The primer
5¢-TCATTGGACTTCCATAAGAACCAGG-3¢was used
for the RT reaction and the primers 5¢-GGAGACAGT
GACAAGTTATCTAGTGCT-3¢and 5¢-TCATTGAG
GAGCAACTGTGTTGG-3¢were used for PCR 1 and 2,
FEBS 2003 Xenopus B¢¢ subunits of PP2A (Eur. J. Biochem. 270) 377
respectively. PCR products were cloned into the pBluescript
II SK
–
vector and sequenced as described.
In vitro
transcription and translation
Full-length cDNAs of the different B¢¢-subunits were
obtained using conventional cloning techniques with the
appropriate restriction enzymes. Truncations of XPR48
cDNA were generated by PCR using Pwo polymerase
(Roche) with different start primers and the specific stop
primer. The resulting cDNA fragments were ligated into the
pBluescript SK
–
vector. GST- and His
6
-tagged-XN73 were
generated by cloning first the full-length cDNA into
the pGex-vector (Amersham-Pharmacia Biotech) and the
pRSET vector (invitrogen), respectively. Subsequently, the
cDNAs with the GST and His
6
-tags were further cloned
into the pBluescript SK
–
vector. [
35
S]Methionine-labelled
translation products were obtained from these templates
using the TNT-coupled reticulocyte lysate system (Pro-
mega) and the appropriate RNA polymerase (T3 and T7).
The translation products were subjected to SDS/PAGE
(12%, w/v) and visualised by autoradiography.
Sequence analysis
Sequence comparisons and alignments were performed
using the
BLAST
tool of NCBI (http://www.ncbi.nlm.nih.
gov/blast/) and the
MULTALIN
alignment program (http://
prodes.toulouse.inra.fr/multalin/multalin.html). Human
genome sequences were obtained by doing
BLAST
searches
against the HTGS database and the human genome site
(http://www.ncbi.nlm.nih.gov/genome/guide/human/).
Monoclonal antibody isolation
cDNAs encoding full length human PP2A PR65a/A or
Ca-subunit were inserted into the pQE 30 bacterial expres-
sion vector (Qiagen) to generate a fusion protein containing
aHis
6
-tag at the N-terminus of the inserted sequence.
PR65/A was expressed and isolated by the methods
described by the vector manufacturer, whereas the recom-
binant C-subunit was insoluble. After isolation of the
inclusion bodies, the protein was solubilized in 0.05% (w/v)
SDS. Young Balb/c ·CBA F
1
crossedmicewereimmu-
nised by subcutaneous injection with 100 lgoffusion
protein emulsified with an equal volume of Titremax Gold
(CytRx Corporation). The immunisation was repeated
three times at 2- to 3-monthly intervals, then the mice
rested for six months. A further 100 lg of fusion protein in
NaCl/P
i
was injected intraperitoneally at 6 and 3 days prior
to sacrifice. All animal experiments were conducted under
the guidelines and regulations contained in the UK Animal
Scientific Procedures Act, 1986. Hybridoma lines were then
established by fusing splenocytes from the immunised
animal with the myeloma line Sp2/0-Ag14 by poly(ethylene
glycol) treatment using conventional procedures [35]. Indi-
vidual wells were screened for antibody production by a
modified dot blot procedure using bacterially expressed
fusion protein [36], and single cells cloned a minimum of
three times before being grown for antibody isolation. Each
monoclonal antibody was screened for specificity by West-
ern blotting against total protein from SDS derived tissue
culture cell lysates. Line C5 1G7 was found to be specific for
the PP2A PR65/A subunit (data not shown), in both human
and Xenopus lysates, which recognises the PR65 aand b
isoforms equally well. The best anti-C monoclonal was
foundtobeF26A10,anditrecognizesPP2A
C
in rabbit,
mouse and Xenopus oocytes and tissues.
GST-pulldown assays and Western blotting
cDNAs of the different B¢¢-subunits (XPR130, XN73 and
XPR70) without UTR sequences were first cloned into a
pGex-vector (Amersham-Pharmacia Biotech). The corres-
ponding GST-fusions and GST alone were subsequently
cloned between the UTR sequences of b-globin into the
pGem high expression vector [37]. Capped synthetic mRNA
transcripts were synthesised by using T7 RNA polymerase
according to the manufacturer’s instructions (mCAP RNA
capping kit, Stratagene). Fifty nL of about 0.5 lgÆlL
)1
of
this synthetic mRNA solution was microinjected into fully
grown stage VI oocytes prepared as described [38]. Oocytes
were incubated for 24 h at 18 C. After incubation,
60 oocytes were homogenized into 150 lL of NaCl/Tris
containing 30 lgÆmL
)1
leupeptin and 0.2 m
M
phenyl-
methylsulfonyl fluoride and a high speed centrifugation
was performed (3 min; 150 000 g; Beckman air driven
ultracentrifuge, rotor A-110/18). The supernatant was
diluted five times in the homogenization buffer containing
1mgÆmL
)1
bovine serum albumin and glutathione–Seph-
arose beads (Amersham-Pharmacia Biotech) were added.
After incubation overnight at 4 C, beads were washed
three times with homogenization buffer containing 0.1% (v/
v) Tween-20 and 0.5
M
NaCl. The glutathione–Sepharose
beads were further eluted by the addition of SDS/PAGE
sample buffer and boiled for 5 min. Western blots were
performed and developed as described in [39] using mono-
clonal antibodies against human PR65 and an enhanced
chemiluminescence detection system (ECL, Amersham-
Pharmacia Biotech).
Semi-quantitative RT-PCR and Southern blotting
Prophase oocytes were prepared as described [38]. Meta-
phase oocytes were derived from prophase oocytes after
addition of 3 l
M
progesterone and overnight incubation of
the oocytes at 18 C. Several Xenopus adult tissues (liver,
gall bladder, spleen, heart, muscle and brain) were excised
from adult female frogs and immediately freeze clamped in
liquid nitrogen. Total RNA from prophase oocytes and
adult tissues was prepared as described [40]. RNA was
further purified using a mini Qiagen RNA purification kit
(Westburg). cDNA was prepared from 1.5 lgoftotalRNA
using the primer 5¢-TGGACTGACAGTTGGAGTAA
GG-3¢for XPR130, the stop primer 5¢-GGAATTCT
AACTCTCGGTTAAGGGGTA-3¢for XN73 or the stop
primer 5¢-TCATTGGACTTCCATAAGAACCAGG-3¢
for XPR70, and 30 U of M-MuLV reverse transcriptase
(Fermentas). Subsequently, the cDNA was purified using a
GFX DNA purification kit (Amersham-Pharmacia Bio-
tech) and PCR was performed in 50 lL using 1 U Pwo-
DNA polymerase (Roche), PCR buffer, 2.5 m
M
MgCl
2
,
200 l
M
of each dNTP, 50 pmol primer. The annealing
of the primers was carried out at 55 C. However, to be
378 I. Stevens et al.(Eur. J. Biochem. 270)FEBS 2003
in the linear DNA amplification range, only 20 amplifi-
cation cycles were performed with the primers 5¢-GTTCC
TGAGCAAAGTCTTCAATG-3¢and 5¢-GGAATTCAG
TGGAAAAACTTTACAT-3¢for XPR130, the primers
5¢-GTTCCTGAGCAAAGTCTTCAATG-3¢and 5¢-GG
AATTCAGTGGAAAAACTTTACAT-3¢for XN73, and
the primers 5¢-GGAATTCATGCCGACCACAACCGTT
TTAAG-3¢and 5¢-GGAGACAGTGACAAGTTATCTA
GTGCT-3¢for XPR70. PCR products were resolved on a
1.5% (w/v) agarose gel and transferred to a Hybond-nylon
membrane (Biorad) by capillary blotting in 2 ·NaCl/Cit.
Pre-hybridization and hybridization was done at 60 Cin
6·NaCl/Cit, 2 ·Denhardt’s reagent, 0.1% (w/v) SDS,
0.05% (w/v) sodium pyrophosphate and 100 lgÆmL
)1
sal-
mon sperm DNA with the primer 5¢-TCTCCAAATCCAA
CAGCAATTT-3¢for XPR130, the primer 5¢-TCTCCA
AATCCAACAGCAATTT-3¢forXN73ortheprimer
5¢-TCATTGAGGAGCAACTGTGTTGG-3¢for XPR70.
Probes were labelled with T4-polynucleotide kinase
(Fermentas) in the presence of [c
32
P]ATP. Membranes were
washed four times for 10 min at 60 Cwith6·NaCl/Cit
and 0.5% (w/v) SDS, and the PCR-DNA fragments were
visualised using a phosphoimager-intensifying screen.
Results
cDNA cloning of PR72/130/B¢¢ subunits from
Xenopus
laevis
prophase oocytes
To clone the PR72/130/B¢¢ subunits of PP2A from Xenopus
laevis, a prophase Xenopus oocytes kgt10 library was
screened using a full-length human PR130 cDNA as a
probe. Out of one million plaques, 27 positive clones were
picked and analysed. These clones could be divided into
three different groups (Fig. 1). The first group (eight clones)
were highly homologous to human PR130 (Fig. 1A).
Unfortunately, only partial clones were isolated, because
even the longest clone lacks information about 142 amino
acids at the 5¢-end when compared with human PR130, and
only one cDNA contained the stop codon and a polyade-
nylation signal. The second group (four clones) could
encode a protein homologous to the N-terminal 73 kDa
part of human PR130 and were therefore designated as
XN73. The open reading frame ended with a stop codon
21 bp downstream of a putative splicing site, which would
translate a protein with an additional stretch of seven amino
acids that are not present in either human PR130 nor human
PR72. The stop codon was followed by several polyadeny-
lation signals (one AATAAA and two ATTAAA motifs)
and a poly(A) tail (Fig. 1B). There was no homology found
between the 3¢-UTR of XN73 and that of XPR130. At the 5¢
end, the longest clone isolated lacked sequences encoding 17
amino acids in comparison with human PR130, so was
probably not complete. The third group contained 15 clones,
all of which were homologous to human PR48 (Fig. 1C).
Because the XPR130 and XN73 cDNAs isolated were
apparently only partial clones missing the 5¢end, we used a
5¢-RACE technique to generate full-length cDNAs. Using
total RNA of prophase Xenopus oocytes as the template, we
were able to isolate DNA fragments after RT-PCR and
several nested PCR attempts. To ensure that we could
generate a PCR fragment long enough to isolate the specific
5¢ends of XN73, a primer close to the 5¢end of the clone
isolated from the library (Fig. 1B) was used in the RT
reaction. The longest RACE fragment obtained contained
an ATG start codon at the same position as in human PR130.
When we used specific primers to amplify only XPR130, we
obtained RACE fragments similar to XPR130 and XN73
clones, but we could not amplify a RACE fragment that
contains the ATG start codon. The predicted amino acid
sequence of the N-terminal region of XPR130 and XN73 are
different in several positions (Fig. 2A). This would normally
indicate that these proteins are encoded by different genes.
However,thegenomeofXenopus laevis has been duplicated
during evolution and so has tetraploid characteristics [41].
Because similar variations were found in the clones isolated
from the oocyte library as well as in the PCR RACE
fragments, it is likely that XPR130 and XN73 are derived
from the same gene and represent splice variants. However,
as genome sequences of Xenopus available in the GenBank
database are still rare, the correctness of this statement
cannot be verified. The full-length cDNA of XPR130 and
XN73 were then used in an in vitro transcription–translation
reaction, and the products separated by SDS/PAGE.
Proteins of about 130 kDa and 50 kDa, respectively, were
obtained (Fig. 2B). Since XN73 runs in SDS/PAGE with an
apparent molecular mass of about 50 kDa instead of the
calculated 72 964 Da, we verified the correctness of the
expected initiation methionine by translating the fusion
protein GST-XN73 and His
6
-XN73. As can be seen in
Fig. 2B, these fusion proteins run at the expected height,
proving that no internal methionine was used as initiation
start in the translation of the wild type mRNA of XN73 and
that the XN73 protein runs with an aberrant molecular mass
in SDS/PAGE. It was previously proven that also PR72, as
isolated from rabbit skeletal muscle runs with an aberrant
molecular mass (about 72 kDa) in SDS/PAGE, different
from its calculated molecular mass (61 096 Da). In this case
the deviation was in the opposite direction [25]. The
underlying physical cause is not known, but it is possible
that both aberrations compensate in the PR130 protein,
running in SDS/PAGE at its expected height.
Together, the data suggest that the group one clones
completed by 5¢-RACE techniques represent the Xenopus
orthologue of human PR130 (51% amino acid identity).
The group two clones encode a protein similar to the
N-terminal region of HPR130 and almost identical to the
N-terminal part of XPR130, but which differs completely at
the C-terminal end, where it abruptly stops seven amino
acids downstream of a putative splicing site. If produced,
this protein would be a new protein never described before
in other species. An alignment of the amino acid sequences
of human PR130 with Xenopus PR130 and Xenopus N73 is
shown in Fig. 2A. cDNA sequences were submitted to the
EMBL GenBank database and have accession numbers
AY043260 for XPR130 and AY043258 for XN73.
Genomic structure of human PR130 favours the
possibility that XN73 is a splice variant
The results obtained from Xenopus cDNAs suggest the
existence of a new potential PP2A B¢¢ subunit which we have
designated as XN73. To determine whether this protein
could exist in other species, we first performed a
BLAST
FEBS 2003 Xenopus B¢¢ subunits of PP2A (Eur. J. Biochem. 270) 379
search with XN73 cDNA as bait. This revealed no similar
EST-clones in other species. We next examined the PR130
gene structure in the human genome database. A blast
search of human PR130 (accession number L12146) against
the human genome NCBI-site revealed a perfect match with
a finished genomic contig (NT_005567.9), designed PPP2R3
A in the human gene nomenclature. The complete gene
spans approximately 375.7 kbp and the human PR130
cDNA consists of 14 exons varying in size between 49 (exon
9) and 2849 nucleotides (exon 14). The splice sites and exon-
intron boundaries are presented in Table 1. All splice donor
and acceptor sites follow the canonical GT/AG rule [42]. A
detailed
MAPVIEW
analysis placed the gene PPP2R3A at the
chromosomal locus 3q22.3.
Detailed analysis of the exon–intron boundaries revealed
that a large part of the 5¢-UTR and the complete N-terminal
Fig. 1. Screening of a prophase Xenopus oocyte kgt10 library identified three classes of clones. (A) Class I: eight clones were isolated that were
homologous to human PR130. All clones were partial as even the longest clone lacks about 142 amino acids at the N-terminal part compared to
human PR130. Arrows shown on the longest clone represent 5¢-RACE strategy of XPR130 as described in materials and methods. (B) Class II: four
clones were isolated that were homologous to the N-terminal 73 kDa fragment of human PR130. The longest clone lacks 17 amino acids at its
N-terminal part compared to the N-terminus of human PR130. The open reading frame ends seven amino acids after the putative splicing site. The
stop codon (vertical line) is followed by a polyadenylation signal and a poly(A) tail. Arrows shown on the longest clone represent 5¢-RACE strategy
of XN73 as described in materials and methods. (C) Class III: 15 clones were isolated that were homologous to human PR48. Three clones have
sequences, upstream of the putative initiation start, which are homologous to sequences of other members of the PR72/130/B¢¢ family. Arrows
shown on the longest clone represent 5¢RACE strategy of XPR70 as described in Materials and methods.
380 I. Stevens et al.(Eur. J. Biochem. 270)FEBS 2003
